Investigations on blood physiology, tissues histology and gene expression profile of Fusarium oxysporum challenged fish
Labeo rohita was challenged with Fusarium oxysporum to demonstrate a thorough immune response. Significant alterations in hematological parameters viz; erythrocyte, hemoglobin, hematocrit, mean corpuscular hemoglobin concentration, mean corpuscular volume, packed cell volume, platelets, and leuk...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20451/1/5.pdf http://journalarticle.ukm.my/20451/ https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html |
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| Summary: | Labeo rohita was challenged with Fusarium oxysporum to demonstrate a thorough immune response. Significant
alterations in hematological parameters viz; erythrocyte, hemoglobin, hematocrit, mean corpuscular hemoglobin
concentration, mean corpuscular volume, packed cell volume, platelets, and leukocytes were observed. Total serum
protein, albumin, globulin, A/G ratio, aspartate aminotransferase, alkaline aminotransferase, alkaline phosphatase
activity, and cholesterol were also altered in the infected fish. Marked histopathological lesions in gills, liver, and kidney
(fusion of gill lamella, necrosis, edema, vacuolization, glomerulus congestion, dilated bowman’s space and renal tubules
alterations) were recorded. Tissue specific gene expression data showed that interleukin was upregulated in infected fish
gills, liver, and kidney but was downregulated in blood. Tumor necrosis factor-α expression was upregulated in blood,
liver, and kidney and was downregulated in gills. Upregulation of interferon-γ was observed in blood, gills, and kidney
but downregulated in liver. All the cellular and molecular findings from this study can be taken as critical biomarkers
in the advancement of diagnostic techniques against fungal infections as a part of wide-ranging programs of systematic
and comprehensive monitoring of fish health and immune system. |
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