Investigation of fly-bacteria’s association on decomposing tissues and antimicrobial evaluation of fly larvae native excretions/secretions
The interaction between flies and microbes on decomposing tissue is mediated by a vast community of bacteria. Currently, the knowledge of this relationship is limited by technological barriers. Besides, the antimicrobial activity of native excretions or secretions (NES) of fly larvae is import...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
2022
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| Online Access: | http://journalarticle.ukm.my/20418/1/51038-190045-1-PB.pdf http://journalarticle.ukm.my/20418/ https://ejournal.ukm.my/serangga/issue/view/1537/showToc |
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| Summary: | The interaction between flies and microbes on decomposing tissue is mediated by a vast
community of bacteria. Currently, the knowledge of this relationship is limited by technological
barriers. Besides, the antimicrobial activity of native excretions or secretions (NES) of fly
larvae is important to be evaluated. The objective of this preliminary study is to understand the
fly-bacteria association on decomposing tissue through bacteria identification along with the
antimicrobial activity of NES. Two fish baits (Rastrelliger brachysoma) weighing 120 grams
each were installed at two locations in Universiti Teknologi MARA, Puncak Alam Campus,
Selangor, Malaysia from 17th April 2021 to 24th April 2021. Specimens of adult flies, larvae,
pupae, and swabs of the bait surface were taken daily. The bacteria colonies were isolated from
four different locations: blowflies’ external surface, blowflies' internal microbiome, larvae
external surface, and bait surface. A total of 23 flies were identified represented by four species
from three families. Chrysomya megacephala (Family: Calliphoridae) was the most abundant
species followed by Sarcophaga sp. (Family: Sarcophagidae), Ophyra spinigera (Family:
Muscidae), and Atherigona orientalis (Family: Muscidae). However, Sarcophaga sp. larvae
were the dominant larva species. Culture-based bacterial investigation showed that both Gram-positive and Gram-negative bacteria were present on the external surface and the internal
microbiome of Ch. megacephala. Meanwhile, temporal changes of the Gram-negative bacteria
group from non-lactose fermenter to lactose fermenter were observed on larvae external surface
samples as well as samples from the decomposing bait. The investigation of NES collected
failed to show any antimicrobial activity. A larger sample size is recommended for the future
antimicrobial properties of NES studies. In conclusion, the data obtained from this preliminary
study can be used as a basic direction for future studies. This study can be extended by using
metagenomic methods to understand more about the bacteria-fly association. |
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