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Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes

Bioactive compounds such as bacteriocins have become potent and promising alternatives to chemical food preservatives for extending food shelf-life and eliminating food loss from microbial spoilage. Enterocin E-760 is a specific bacterocin belonging to class II that possesses broad spectrum antibact...

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Main Authors: Nguyen, Khanh Hoang Viet, To, Lan Anh, Luong, Kim Phuong
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/19742/1/5.pdf
http://journalarticle.ukm.my/19742/
https://www.ukm.my/jsm/malay_journals/jilid51bil6_2022/KandunganJilid51Bil6_2022.html
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spelling my-ukm.journal.197422022-09-19T06:43:24Z http://journalarticle.ukm.my/19742/ Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes Nguyen, Khanh Hoang Viet To, Lan Anh Luong, Kim Phuong Bioactive compounds such as bacteriocins have become potent and promising alternatives to chemical food preservatives for extending food shelf-life and eliminating food loss from microbial spoilage. Enterocin E-760 is a specific bacterocin belonging to class II that possesses broad spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria. In this study, the enterocin E-760 gene was fused to a His-tag and cloned into the expression vector, pPICzαA, and transformed into Escherichia coli DH5α cells. The recombinant plasmid was isolated, linearised and transformed into competent Pichia pastoris X33 cells using electroporation. The Pichia transformants were determined using PCR and expressed under methanol induction with the highest antibacterial activity of culture supernatants reaching 40 AU/mL. Enterocin E-760 exhibited a molecular weight of approximately 5.5 kDa and was detected directly on a Tricine SDS-PAGE gel containing Listeria monocytogenes ATCC 35152 after ethanol precipitation at a concentration ranging from 30% to 70%. This study represented the initial stages of research into using enterocin as a biopreservative in food processing. Penerbit Universiti Kebangsaan Malaysia 2022-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19742/1/5.pdf Nguyen, Khanh Hoang Viet and To, Lan Anh and Luong, Kim Phuong (2022) Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes. Sains Malaysiana, 51 (6). pp. 1667-1676. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil6_2022/KandunganJilid51Bil6_2022.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Bioactive compounds such as bacteriocins have become potent and promising alternatives to chemical food preservatives for extending food shelf-life and eliminating food loss from microbial spoilage. Enterocin E-760 is a specific bacterocin belonging to class II that possesses broad spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria. In this study, the enterocin E-760 gene was fused to a His-tag and cloned into the expression vector, pPICzαA, and transformed into Escherichia coli DH5α cells. The recombinant plasmid was isolated, linearised and transformed into competent Pichia pastoris X33 cells using electroporation. The Pichia transformants were determined using PCR and expressed under methanol induction with the highest antibacterial activity of culture supernatants reaching 40 AU/mL. Enterocin E-760 exhibited a molecular weight of approximately 5.5 kDa and was detected directly on a Tricine SDS-PAGE gel containing Listeria monocytogenes ATCC 35152 after ethanol precipitation at a concentration ranging from 30% to 70%. This study represented the initial stages of research into using enterocin as a biopreservative in food processing.
format Article
author Nguyen, Khanh Hoang Viet
To, Lan Anh
Luong, Kim Phuong
spellingShingle Nguyen, Khanh Hoang Viet
To, Lan Anh
Luong, Kim Phuong
Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
author_facet Nguyen, Khanh Hoang Viet
To, Lan Anh
Luong, Kim Phuong
author_sort Nguyen, Khanh Hoang Viet
title Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
title_short Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
title_full Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
title_fullStr Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
title_full_unstemmed Expression of the recombinant enterocin E-760 in Pichia pastoris X33 and its antimicrobial activity towards Listeria monocytogenes
title_sort expression of the recombinant enterocin e-760 in pichia pastoris x33 and its antimicrobial activity towards listeria monocytogenes
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/19742/1/5.pdf
http://journalarticle.ukm.my/19742/
https://www.ukm.my/jsm/malay_journals/jilid51bil6_2022/KandunganJilid51Bil6_2022.html
_version_ 1744649874065850368
score 13.211869

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