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Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA

Durians in North Maluku (Durio spp.) have many local names which are taxonomically ambiguous. These ambiguous can be solved with selecting suitable barcodes for identification. It has been identified for first time using molecular technique. DNA barcode is one technique that can be used for specie...

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Main Authors: Sundari,, Wahyudi, Didik, Arumingtyas, Estri Laras, Hakim, Luchman, Rodiyati Azrianingsih,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/19356/1/48_05_09.pdf
http://journalarticle.ukm.my/19356/
https://jms.mabjournal.com/index.php/mab/issue/view/8
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spelling my-ukm.journal.193562022-08-16T00:48:51Z http://journalarticle.ukm.my/19356/ Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA Sundari, Wahyudi, Didik Arumingtyas, Estri Laras Hakim, Luchman Rodiyati Azrianingsih, Durians in North Maluku (Durio spp.) have many local names which are taxonomically ambiguous. These ambiguous can be solved with selecting suitable barcodes for identification. It has been identified for first time using molecular technique. DNA barcode is one technique that can be used for species identification using standard DNA sequences. The objectives of this study is to evaluate the effectiveness of three DNA barcodes to identify and discriminate species of local Durian in North Maluku. A total of 36 samples representing Durian with some local names were collected from three islands (Ternate, Tidore, Jailolo) and were identified using three DNA barcode: matK gene, ITS regions and trnL intron. ITS showed the highest sequencing success, the highest variable site and parsimony informative, most effective for identification using BLASTN NCBI and resulted the most reliable phylogenetic tree. Moreover ITS also has the highest distribution of intraspecific K2P distance (> 0.02) that make ITS can be used as marker to discriminate the Durian. In other hand matK showed the lowest sequencing success but more effective for identification than trnL. In general the results of this research informs that among the three DNA barcodes, i.e. matK, ITS, and trnL, ITS region is the most effective for identifying and discriminating Durio spp. Penerbit Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19356/1/48_05_09.pdf Sundari, and Wahyudi, Didik and Arumingtyas, Estri Laras and Hakim, Luchman and Rodiyati Azrianingsih, (2019) Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA. Malaysian Applied Biology, 48 (5). pp. 77-87. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/8
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Durians in North Maluku (Durio spp.) have many local names which are taxonomically ambiguous. These ambiguous can be solved with selecting suitable barcodes for identification. It has been identified for first time using molecular technique. DNA barcode is one technique that can be used for species identification using standard DNA sequences. The objectives of this study is to evaluate the effectiveness of three DNA barcodes to identify and discriminate species of local Durian in North Maluku. A total of 36 samples representing Durian with some local names were collected from three islands (Ternate, Tidore, Jailolo) and were identified using three DNA barcode: matK gene, ITS regions and trnL intron. ITS showed the highest sequencing success, the highest variable site and parsimony informative, most effective for identification using BLASTN NCBI and resulted the most reliable phylogenetic tree. Moreover ITS also has the highest distribution of intraspecific K2P distance (> 0.02) that make ITS can be used as marker to discriminate the Durian. In other hand matK showed the lowest sequencing success but more effective for identification than trnL. In general the results of this research informs that among the three DNA barcodes, i.e. matK, ITS, and trnL, ITS region is the most effective for identifying and discriminating Durio spp.
format Article
author Sundari,
Wahyudi, Didik
Arumingtyas, Estri Laras
Hakim, Luchman
Rodiyati Azrianingsih,
spellingShingle Sundari,
Wahyudi, Didik
Arumingtyas, Estri Laras
Hakim, Luchman
Rodiyati Azrianingsih,
Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
author_facet Sundari,
Wahyudi, Didik
Arumingtyas, Estri Laras
Hakim, Luchman
Rodiyati Azrianingsih,
author_sort Sundari,
title Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
title_short Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
title_full Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
title_fullStr Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
title_full_unstemmed Assessment for identification of genus Durio spp. from North Maluku Indonesia using three barcode DNA
title_sort assessment for identification of genus durio spp. from north maluku indonesia using three barcode dna
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/19356/1/48_05_09.pdf
http://journalarticle.ukm.my/19356/
https://jms.mabjournal.com/index.php/mab/issue/view/8
_version_ 1743107989484601344
score 13.211869

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