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Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro

Dehydroabietic acid (DHA) is an analog of abietic acid (AA) for cardiovascular disease prevention, known to act pharmacologically against aging, inflammation, bacterial infections and cancer. The current research investigated the molecular mechanisms of DHA on the adhesiveness of endothelial leukocy...

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Main Authors: Liang, Xiao, Wu, Yan
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/19281/1/19.pdf
http://journalarticle.ukm.my/19281/
https://www.ukm.my/jsm/malay_journals/jilid51bil4_2022/KandunganJilid51Bil4_2022.html
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spelling my-ukm.journal.192812022-08-08T06:44:22Z http://journalarticle.ukm.my/19281/ Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro Liang, Xiao Wu, Yan Dehydroabietic acid (DHA) is an analog of abietic acid (AA) for cardiovascular disease prevention, known to act pharmacologically against aging, inflammation, bacterial infections and cancer. The current research investigated the molecular mechanisms of DHA on the adhesiveness of endothelial leukocytes and activation of NF-κB in TNF-α treated HAEC and ApoE−/− mice in experimental atherosclerosis. The HAECs were tested for toxicity using MTT assay at various DHA concentrations. The cell-surface expression of CAM against endothelial leukocyte adhesion molecule-1, ICAM -1, or human VCAM-1 was determined by the ELISA test, followed by western blot analysis. Endothelial cell-leukocyte adhesion assay involving U937 cells was carried out followed by the determination of NF-κB p65 expression. ApoE−/− mice were fed with a high cholesterol diet every day followed by oral administration of DHA (10 and 20 mg/kg/day). The DHA (5 and 10 mM) substantially reduced (p < 0.05) human (U937) cell lines binding to TNF-α activated human endothelial aortic cells. The assays involving the 32P-labelled NF-kB as a probe demonstrated that DHA pre-treatment decreased the shifted bands density following the stimulation of TNF-α. Nuclear extracts immune blot assessment and immune fluorescence staining showed a significant decrease (p < 0.05) in the NF-κB p65 concentration in the nuclei with DHA treated human endothelial aortic cells. Together these findings indicate that DHA inhibits nuclear trans localization of TNF-α-induced NF-κB p65, and thus considerably suppresses (p < 0.05) the VCAM-1 expression, which contributes to lower leukocyte adherence suggesting that DHA helps in preventing inflammatory atherosclerosis in vivo. Penerbit Universiti Kebangsaan Malaysia 2022-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/19281/1/19.pdf Liang, Xiao and Wu, Yan (2022) Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro. Sains Malaysiana, 51 (4). pp. 1181-1196. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil4_2022/KandunganJilid51Bil4_2022.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Dehydroabietic acid (DHA) is an analog of abietic acid (AA) for cardiovascular disease prevention, known to act pharmacologically against aging, inflammation, bacterial infections and cancer. The current research investigated the molecular mechanisms of DHA on the adhesiveness of endothelial leukocytes and activation of NF-κB in TNF-α treated HAEC and ApoE−/− mice in experimental atherosclerosis. The HAECs were tested for toxicity using MTT assay at various DHA concentrations. The cell-surface expression of CAM against endothelial leukocyte adhesion molecule-1, ICAM -1, or human VCAM-1 was determined by the ELISA test, followed by western blot analysis. Endothelial cell-leukocyte adhesion assay involving U937 cells was carried out followed by the determination of NF-κB p65 expression. ApoE−/− mice were fed with a high cholesterol diet every day followed by oral administration of DHA (10 and 20 mg/kg/day). The DHA (5 and 10 mM) substantially reduced (p < 0.05) human (U937) cell lines binding to TNF-α activated human endothelial aortic cells. The assays involving the 32P-labelled NF-kB as a probe demonstrated that DHA pre-treatment decreased the shifted bands density following the stimulation of TNF-α. Nuclear extracts immune blot assessment and immune fluorescence staining showed a significant decrease (p < 0.05) in the NF-κB p65 concentration in the nuclei with DHA treated human endothelial aortic cells. Together these findings indicate that DHA inhibits nuclear trans localization of TNF-α-induced NF-κB p65, and thus considerably suppresses (p < 0.05) the VCAM-1 expression, which contributes to lower leukocyte adherence suggesting that DHA helps in preventing inflammatory atherosclerosis in vivo.
format Article
author Liang, Xiao
Wu, Yan
spellingShingle Liang, Xiao
Wu, Yan
Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
author_facet Liang, Xiao
Wu, Yan
author_sort Liang, Xiao
title Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
title_short Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
title_full Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
title_fullStr Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
title_full_unstemmed Dehydroabietic acid attenuates atherosclerosis in apolipoprotein E-deficient mice and VCAM-1 expressions in-vitro
title_sort dehydroabietic acid attenuates atherosclerosis in apolipoprotein e-deficient mice and vcam-1 expressions in-vitro
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/19281/1/19.pdf
http://journalarticle.ukm.my/19281/
https://www.ukm.my/jsm/malay_journals/jilid51bil4_2022/KandunganJilid51Bil4_2022.html
_version_ 1740826835401310208
score 13.211869

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