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Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)

Identification of a tick species, Dermacentor compactus (Acari: Ixodidae) from Malaysia is still debatable in many aspects and remains poorly studied, especially on the immature stages. This study was conducted to characterize morphologically and molecularly of the D. compactus ticks collected fr...

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Main Authors: Ernieenor Faraliana Che Lah,, Ai Takano,, Ernna George,, Mariana Ahamad,
Format: Article
Language:English
Published: Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18969/1/45575-163573-1-PB.pdf
http://journalarticle.ukm.my/18969/
https://ejournal.ukm.my/serangga/issue/view/1414/showToc
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spelling my-ukm.journal.189692022-07-20T04:46:17Z http://journalarticle.ukm.my/18969/ Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae) Ernieenor Faraliana Che Lah, Ai Takano, Ernna George, Mariana Ahamad, Identification of a tick species, Dermacentor compactus (Acari: Ixodidae) from Malaysia is still debatable in many aspects and remains poorly studied, especially on the immature stages. This study was conducted to characterize morphologically and molecularly of the D. compactus ticks collected from a forest reserve near the Forest Research Institute of Malaysia (FRIM). A total of three adults (two females and one male) of questing D. compactus ticks on lower vegetation or shrubs were collected by handpicking. Species identification was conducted based on specific illustrated taxonomic keys, and molecular characteristics of D. compactus was analyzed based on mitochondrial 16S rDNA gene (mt-rrs). A neighbor-joining tree was constructed to clarify the genetic variation of the D. compactus. Clustering analysis using mtrrs gene revealed that the sequences of the D. compactus were formed in a monophyletic clade supported with 100% bootstrap value. Furthermore, a low intraspecific variation (1%) was observed amongst the species of D. compactus. Meanwhile, genetic distance of the D. compactus also supported that the species is genetically distinct from the other Dermacentor species with a high interspecific value (>13%). This study reported the first of 16S sequences of the D. compactus from Malaysia. These present results will contribute to the existing genotypic data of this species from Malaysia, thus merit further investigation as potential vector that related to any tick-borne diseases. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18969/1/45575-163573-1-PB.pdf Ernieenor Faraliana Che Lah, and Ai Takano, and Ernna George, and Mariana Ahamad, (2021) Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae). Serangga, 26 (3). pp. 13-28. ISSN 1394-5130 https://ejournal.ukm.my/serangga/issue/view/1414/showToc
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Identification of a tick species, Dermacentor compactus (Acari: Ixodidae) from Malaysia is still debatable in many aspects and remains poorly studied, especially on the immature stages. This study was conducted to characterize morphologically and molecularly of the D. compactus ticks collected from a forest reserve near the Forest Research Institute of Malaysia (FRIM). A total of three adults (two females and one male) of questing D. compactus ticks on lower vegetation or shrubs were collected by handpicking. Species identification was conducted based on specific illustrated taxonomic keys, and molecular characteristics of D. compactus was analyzed based on mitochondrial 16S rDNA gene (mt-rrs). A neighbor-joining tree was constructed to clarify the genetic variation of the D. compactus. Clustering analysis using mtrrs gene revealed that the sequences of the D. compactus were formed in a monophyletic clade supported with 100% bootstrap value. Furthermore, a low intraspecific variation (1%) was observed amongst the species of D. compactus. Meanwhile, genetic distance of the D. compactus also supported that the species is genetically distinct from the other Dermacentor species with a high interspecific value (>13%). This study reported the first of 16S sequences of the D. compactus from Malaysia. These present results will contribute to the existing genotypic data of this species from Malaysia, thus merit further investigation as potential vector that related to any tick-borne diseases.
format Article
author Ernieenor Faraliana Che Lah,
Ai Takano,
Ernna George,
Mariana Ahamad,
spellingShingle Ernieenor Faraliana Che Lah,
Ai Takano,
Ernna George,
Mariana Ahamad,
Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
author_facet Ernieenor Faraliana Che Lah,
Ai Takano,
Ernna George,
Mariana Ahamad,
author_sort Ernieenor Faraliana Che Lah,
title Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
title_short Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
title_full Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
title_fullStr Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
title_full_unstemmed Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
title_sort integrated phenotypic and genotypic approach for characterization of local questing hard tick, dermacentor compactus (acari: ixodidae)
publisher Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/18969/1/45575-163573-1-PB.pdf
http://journalarticle.ukm.my/18969/
https://ejournal.ukm.my/serangga/issue/view/1414/showToc
_version_ 1739829172030668800
score 13.211869

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