Integrated phenotypic and genotypic approach for characterization of local questing hard tick, Dermacentor compactus (Acari: Ixodidae)
Identification of a tick species, Dermacentor compactus (Acari: Ixodidae) from Malaysia is still debatable in many aspects and remains poorly studied, especially on the immature stages. This study was conducted to characterize morphologically and molecularly of the D. compactus ticks collected fr...
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| 主要な著者: | , , , |
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| フォーマット: | 論文 |
| 言語: | English |
| 出版事項: |
Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
2021
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| オンライン･アクセス: | http://journalarticle.ukm.my/18969/1/45575-163573-1-PB.pdf http://journalarticle.ukm.my/18969/ https://ejournal.ukm.my/serangga/issue/view/1414/showToc |
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| 要約: | Identification of a tick species, Dermacentor compactus (Acari: Ixodidae) from Malaysia is still
debatable in many aspects and remains poorly studied, especially on the immature stages. This
study was conducted to characterize morphologically and molecularly of the D. compactus ticks
collected from a forest reserve near the Forest Research Institute of Malaysia (FRIM). A total
of three adults (two females and one male) of questing D. compactus ticks on lower vegetation
or shrubs were collected by handpicking. Species identification was conducted based on
specific illustrated taxonomic keys, and molecular characteristics of D. compactus was
analyzed based on mitochondrial 16S rDNA gene (mt-rrs). A neighbor-joining tree was
constructed to clarify the genetic variation of the D. compactus. Clustering analysis using mtrrs
gene revealed that the sequences of the D. compactus were formed in a monophyletic clade
supported with 100% bootstrap value. Furthermore, a low intraspecific variation (1%) was
observed amongst the species of D. compactus. Meanwhile, genetic distance of the D.
compactus also supported that the species is genetically distinct from the other Dermacentor
species with a high interspecific value (>13%). This study reported the first of 16S sequences
of the D. compactus from Malaysia. These present results will contribute to the existing
genotypic data of this species from Malaysia, thus merit further investigation as potential vector
that related to any tick-borne diseases. |
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