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Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer

Tocotrienols are reported to possess anticancer activities. Recently we showed that the anticancer effects of tocotrienolrich fraction (TRF) may be through inhibition of the special AT-rich sequence-binding protein 1 (SATB1) gene in a syngeneic mouse model of breast cancer (BC). The present study...

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Main Authors: Sitti Rahma Abdul Hafid,, Ammu Kutty Radhakrishnan,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18893/1/145-161%2B%2B%2BSitti%2BRahma%2BAbdul%2BHafid.pdf
http://journalarticle.ukm.my/18893/
https://jms.mabjournal.com/index.php/mab/issue/view/43
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spelling my-ukm.journal.188932022-07-05T07:53:09Z http://journalarticle.ukm.my/18893/ Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer Sitti Rahma Abdul Hafid, Ammu Kutty Radhakrishnan, Tocotrienols are reported to possess anticancer activities. Recently we showed that the anticancer effects of tocotrienolrich fraction (TRF) may be through inhibition of the special AT-rich sequence-binding protein 1 (SATB1) gene in a syngeneic mouse model of breast cancer (BC). The present study aims to further explore the role of the SATB1 gene in murine BC cells. The expression of the SATB1 gene in the 4T1 murine BC cells was partially knocked down (SATB1-4T1) using the short hairpin RNA (shRNA) technology and these cells were injected into the mammary pads of mice. Control groups were injected with wild-type 4T1 (WT 4T1) cells. When the tumour was palpable, half of the mice in both groups were fed daily with 1 mg TRF and received intraperitoneal injections of dendritic cells pulsed with tumour lysate (DC+TL) once a week for three weeks. The tumour incidence in mice injected with the SATB1-4T1 cells was reduced (p<0.05) and this effect was independent of TRF supplementation. However, in mice injected with WT-4T1, there was inhibition of tumour growth (p<0.05) only in the group fed with TRF. In addition, the expression of S1004A and mutant P53 genes were suppressed in tumours from animals that were injected with the SATB1-4T1 cells, irrespective of TRF supplementation; which was also observed in tumours from mice injected with WT 4T1 cells and fed with TRF. These findings suggest that TRF may work through the SATB1 pathway. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18893/1/145-161%2B%2B%2BSitti%2BRahma%2BAbdul%2BHafid.pdf Sitti Rahma Abdul Hafid, and Ammu Kutty Radhakrishnan, (2021) Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer. Malaysian Applied Biology, 50 (3). pp. 145-161. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/43
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Tocotrienols are reported to possess anticancer activities. Recently we showed that the anticancer effects of tocotrienolrich fraction (TRF) may be through inhibition of the special AT-rich sequence-binding protein 1 (SATB1) gene in a syngeneic mouse model of breast cancer (BC). The present study aims to further explore the role of the SATB1 gene in murine BC cells. The expression of the SATB1 gene in the 4T1 murine BC cells was partially knocked down (SATB1-4T1) using the short hairpin RNA (shRNA) technology and these cells were injected into the mammary pads of mice. Control groups were injected with wild-type 4T1 (WT 4T1) cells. When the tumour was palpable, half of the mice in both groups were fed daily with 1 mg TRF and received intraperitoneal injections of dendritic cells pulsed with tumour lysate (DC+TL) once a week for three weeks. The tumour incidence in mice injected with the SATB1-4T1 cells was reduced (p<0.05) and this effect was independent of TRF supplementation. However, in mice injected with WT-4T1, there was inhibition of tumour growth (p<0.05) only in the group fed with TRF. In addition, the expression of S1004A and mutant P53 genes were suppressed in tumours from animals that were injected with the SATB1-4T1 cells, irrespective of TRF supplementation; which was also observed in tumours from mice injected with WT 4T1 cells and fed with TRF. These findings suggest that TRF may work through the SATB1 pathway.
format Article
author Sitti Rahma Abdul Hafid,
Ammu Kutty Radhakrishnan,
spellingShingle Sitti Rahma Abdul Hafid,
Ammu Kutty Radhakrishnan,
Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
author_facet Sitti Rahma Abdul Hafid,
Ammu Kutty Radhakrishnan,
author_sort Sitti Rahma Abdul Hafid,
title Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
title_short Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
title_full Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
title_fullStr Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
title_full_unstemmed Elucidating the role of the SATB1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
title_sort elucidating the role of the satb1 gene in breast cancer carcinogenesis in the presence or absence of tocotrienol-rich fraction : evidence from a syngeneic mouse model of breast cancer
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/18893/1/145-161%2B%2B%2BSitti%2BRahma%2BAbdul%2BHafid.pdf
http://journalarticle.ukm.my/18893/
https://jms.mabjournal.com/index.php/mab/issue/view/43
_version_ 1738511362602041344
score 13.211869

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