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Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand

Rhododendron arboreum Sm. has wide applications in food and beverage, medicines, and cosmetics because the plant contains bioactive phytochemical components. In Thailand, other than for horticultural purposes, scant literature describes the potential applications of this Rhododendron species. Phyt...

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Main Authors: Supanida Winitchai,, Siriluck Liengprayoon,, Warawut Suphamitmongkol,, Naddamas Tomorn,, Jatuporn Chaiyut,, Tuksin Lerksamran,, Yutthana Banchong,, Piyapat Trisonthi,, Safiah Saah,, Natedao Musigamart,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18878/1/023-037%2B%2B%2BNatedao%2BMusigamart.pdf
http://journalarticle.ukm.my/18878/
https://jms.mabjournal.com/index.php/mab/issue/view/43
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spelling my-ukm.journal.188782022-07-05T07:37:33Z http://journalarticle.ukm.my/18878/ Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand Supanida Winitchai, Siriluck Liengprayoon, Warawut Suphamitmongkol, Naddamas Tomorn, Jatuporn Chaiyut, Tuksin Lerksamran, Yutthana Banchong, Piyapat Trisonthi, Safiah Saah, Natedao Musigamart, Rhododendron arboreum Sm. has wide applications in food and beverage, medicines, and cosmetics because the plant contains bioactive phytochemical components. In Thailand, other than for horticultural purposes, scant literature describes the potential applications of this Rhododendron species. Phytochemical composition, biological activity, and cell cytotoxicity of R. arboreum Sm. flower petal and leaf extracts were determined. Ethanolic extracts of fresh and dried flower petals (FF and DF, respectively) and dried leaves (DL) of R. arboreum were prepared by maceration with 60% ethanol for 7 days. Extraction yields of both FF and DF were higher than DL (19.22, 16.76, and 8.50% for FF, DF, and DL, respectively). Preliminary qualitative phytochemical screening showed different compositions in diverse plant parts. Saponins and tannins were present in every extract, with anthraquinones detected only in flowers and terpenoids only in leaves. Total phenolic (TPC) and flavonoid contents (TFC) were highest in DL (405.21 mg gallic acid equivalent and 127.30 mg catechin equivalent per g of dry extract, respectively), followed by DF and FF. Antioxidant properties were determined using two radical scavenging assays as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethyl-benzthiazoline-6-sulfonic acid (ABTS). Results indicated that all extracts exhibited better inhibitory activity against DPPH radical than ABTS radical as evidenced by lower IC50 range 24.65-48.15 ìg/mL for DPPH and 65.19-76.36 ìg/mL for ABTS, respectively. A positive correlation coefficient between the two antioxidant assays and TPC and TFC of Rhododendron extracts indicated antioxidant potential distributed in both components. No cytotoxicity was recorded in the three extracts, with concentrations less than 500 μg/ mL for both 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) and CellTiter-Blue® assay. In vitro studies exhibited dose-dependent and strong anti-melanogenic and anti-inflammatory activities ranging from 50 to 250 μg/mL for FF, DF, and DL. Results identified various bioactive constituents with potential biological activity (antioxidant, antiinflammatory, and anti-melanogenic), while non-cytotoxicity in R. arboreum flower and leaf extracts suggested the possibility of further applications as a functional ingredient in cosmetics. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18878/1/023-037%2B%2B%2BNatedao%2BMusigamart.pdf Supanida Winitchai, and Siriluck Liengprayoon, and Warawut Suphamitmongkol, and Naddamas Tomorn, and Jatuporn Chaiyut, and Tuksin Lerksamran, and Yutthana Banchong, and Piyapat Trisonthi, and Safiah Saah, and Natedao Musigamart, (2021) Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand. Malaysian Applied Biology, 50 (3). pp. 23-37. ISSN 0126-8643 https://jms.mabjournal.com/index.php/mab/issue/view/43
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Rhododendron arboreum Sm. has wide applications in food and beverage, medicines, and cosmetics because the plant contains bioactive phytochemical components. In Thailand, other than for horticultural purposes, scant literature describes the potential applications of this Rhododendron species. Phytochemical composition, biological activity, and cell cytotoxicity of R. arboreum Sm. flower petal and leaf extracts were determined. Ethanolic extracts of fresh and dried flower petals (FF and DF, respectively) and dried leaves (DL) of R. arboreum were prepared by maceration with 60% ethanol for 7 days. Extraction yields of both FF and DF were higher than DL (19.22, 16.76, and 8.50% for FF, DF, and DL, respectively). Preliminary qualitative phytochemical screening showed different compositions in diverse plant parts. Saponins and tannins were present in every extract, with anthraquinones detected only in flowers and terpenoids only in leaves. Total phenolic (TPC) and flavonoid contents (TFC) were highest in DL (405.21 mg gallic acid equivalent and 127.30 mg catechin equivalent per g of dry extract, respectively), followed by DF and FF. Antioxidant properties were determined using two radical scavenging assays as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis(3-ethyl-benzthiazoline-6-sulfonic acid (ABTS). Results indicated that all extracts exhibited better inhibitory activity against DPPH radical than ABTS radical as evidenced by lower IC50 range 24.65-48.15 ìg/mL for DPPH and 65.19-76.36 ìg/mL for ABTS, respectively. A positive correlation coefficient between the two antioxidant assays and TPC and TFC of Rhododendron extracts indicated antioxidant potential distributed in both components. No cytotoxicity was recorded in the three extracts, with concentrations less than 500 μg/ mL for both 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) and CellTiter-Blue® assay. In vitro studies exhibited dose-dependent and strong anti-melanogenic and anti-inflammatory activities ranging from 50 to 250 μg/mL for FF, DF, and DL. Results identified various bioactive constituents with potential biological activity (antioxidant, antiinflammatory, and anti-melanogenic), while non-cytotoxicity in R. arboreum flower and leaf extracts suggested the possibility of further applications as a functional ingredient in cosmetics.
format Article
author Supanida Winitchai,
Siriluck Liengprayoon,
Warawut Suphamitmongkol,
Naddamas Tomorn,
Jatuporn Chaiyut,
Tuksin Lerksamran,
Yutthana Banchong,
Piyapat Trisonthi,
Safiah Saah,
Natedao Musigamart,
spellingShingle Supanida Winitchai,
Siriluck Liengprayoon,
Warawut Suphamitmongkol,
Naddamas Tomorn,
Jatuporn Chaiyut,
Tuksin Lerksamran,
Yutthana Banchong,
Piyapat Trisonthi,
Safiah Saah,
Natedao Musigamart,
Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
author_facet Supanida Winitchai,
Siriluck Liengprayoon,
Warawut Suphamitmongkol,
Naddamas Tomorn,
Jatuporn Chaiyut,
Tuksin Lerksamran,
Yutthana Banchong,
Piyapat Trisonthi,
Safiah Saah,
Natedao Musigamart,
author_sort Supanida Winitchai,
title Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
title_short Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
title_full Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
title_fullStr Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
title_full_unstemmed Phytochemical composition and biological activities of crude extract from flowers and leaves of Rhododendron arboreum Sm. from Northern Thailand
title_sort phytochemical composition and biological activities of crude extract from flowers and leaves of rhododendron arboreum sm. from northern thailand
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/18878/1/023-037%2B%2B%2BNatedao%2BMusigamart.pdf
http://journalarticle.ukm.my/18878/
https://jms.mabjournal.com/index.php/mab/issue/view/43
_version_ 1738511361010302976
score 13.223943

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