Cover Image

Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis

Food regimens and herbs have been a target of scientific research in treating cancer. This study aimed to elucidate the effect of Zingiber officinale (ginger) and Piper betle (PB) alone or in combination on the colorectal cancer cell lines HCT116 and HT29. The chemopreventive effect of these extract...

Full description

Saved in:
Bibliographic Details
Main Authors: Yasmin Anum Mohd Yusof,, Shailah Abdullah,, Nur Fatin Nabilah Mohd Sahardi,, Wan Zurinah Wan Ngah,, Suzana Makpol,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/18360/1/18.pdf
http://journalarticle.ukm.my/18360/
https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.18360
record_format eprints
spelling my-ukm.journal.183602022-04-11T07:16:51Z http://journalarticle.ukm.my/18360/ Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis Yasmin Anum Mohd Yusof, Shailah Abdullah, Nur Fatin Nabilah Mohd Sahardi, Wan Zurinah Wan Ngah, Suzana Makpol, Food regimens and herbs have been a target of scientific research in treating cancer. This study aimed to elucidate the effect of Zingiber officinale (ginger) and Piper betle (PB) alone or in combination on the colorectal cancer cell lines HCT116 and HT29. The chemopreventive effect of these extracts was determined by performing cell viability assay, cell cycle analysis, apoptosis analysis, and caspase 3 and 8 activity assays in HCT116 and p53-deficient HT29 cells. Ginger and PB extracts inhibited the proliferation of both cancer cell lines dose-dependently, but the combined extracts inhibited the growth of cancer cells synergistically with an IC50 PB plus ginger < IC50 PB < IC50 ginger. Similarly, the effect of the combined extracts on apoptosis of both cancer cell lines was higher than that of each individual extract. Consistent with the enhancement of caspase activity, both extracts increased the expression of BAX protein, while Bcl-2 protein was decreased. Flow cytometry analysis indicated that the combined extracts arrested a higher percentage of colon cancer cells at the G0/G1 phase with a concomitant decrease in cells in the S phase, indicating that the combined extracts inhibited human colon cancer cell growth by cell cycle arrest at the G0/G1 phase. The inhibitory effect of these combined extracts on cellular proliferation and the induction of apoptosis was synergistic, showing a significant combination index (CI) value < 1.0. The combined extracts of ginger and PB may serve as a potential chemopreventive agent against colon cancer through the induction of caspase-mediated apoptosis. Penerbit Universiti Kebangsaan Malaysia 2022-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18360/1/18.pdf Yasmin Anum Mohd Yusof, and Shailah Abdullah, and Nur Fatin Nabilah Mohd Sahardi, and Wan Zurinah Wan Ngah, and Suzana Makpol, (2022) Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis. Sains Malaysiana, 51 (1). pp. 217-237. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Food regimens and herbs have been a target of scientific research in treating cancer. This study aimed to elucidate the effect of Zingiber officinale (ginger) and Piper betle (PB) alone or in combination on the colorectal cancer cell lines HCT116 and HT29. The chemopreventive effect of these extracts was determined by performing cell viability assay, cell cycle analysis, apoptosis analysis, and caspase 3 and 8 activity assays in HCT116 and p53-deficient HT29 cells. Ginger and PB extracts inhibited the proliferation of both cancer cell lines dose-dependently, but the combined extracts inhibited the growth of cancer cells synergistically with an IC50 PB plus ginger < IC50 PB < IC50 ginger. Similarly, the effect of the combined extracts on apoptosis of both cancer cell lines was higher than that of each individual extract. Consistent with the enhancement of caspase activity, both extracts increased the expression of BAX protein, while Bcl-2 protein was decreased. Flow cytometry analysis indicated that the combined extracts arrested a higher percentage of colon cancer cells at the G0/G1 phase with a concomitant decrease in cells in the S phase, indicating that the combined extracts inhibited human colon cancer cell growth by cell cycle arrest at the G0/G1 phase. The inhibitory effect of these combined extracts on cellular proliferation and the induction of apoptosis was synergistic, showing a significant combination index (CI) value < 1.0. The combined extracts of ginger and PB may serve as a potential chemopreventive agent against colon cancer through the induction of caspase-mediated apoptosis.
format Article
author Yasmin Anum Mohd Yusof,
Shailah Abdullah,
Nur Fatin Nabilah Mohd Sahardi,
Wan Zurinah Wan Ngah,
Suzana Makpol,
spellingShingle Yasmin Anum Mohd Yusof,
Shailah Abdullah,
Nur Fatin Nabilah Mohd Sahardi,
Wan Zurinah Wan Ngah,
Suzana Makpol,
Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
author_facet Yasmin Anum Mohd Yusof,
Shailah Abdullah,
Nur Fatin Nabilah Mohd Sahardi,
Wan Zurinah Wan Ngah,
Suzana Makpol,
author_sort Yasmin Anum Mohd Yusof,
title Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
title_short Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
title_full Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
title_fullStr Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
title_full_unstemmed Zingiber officinale roscoe and Piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
title_sort zingiber officinale roscoe and piper betleextracts enhanced the chemopreventive effect against colon cancer cells by targeting caspase-mediated apoptosis
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/18360/1/18.pdf
http://journalarticle.ukm.my/18360/
https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
_version_ 1731226526376001536
score 13.211869

Similar Items