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Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia

Wolbachia has the ability to cause reproductive abnormalities in infected hosts including cytoplasmic incompatibility (CI). CI is activated when there are multiple Wolbachia supergroups or strains infection present in insect populations. Wolbachia-transinfected mosquitoes have been used widely in so...

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Main Authors: Noor Shazleen Husnie Mohd Mohtar,, Emelia Osman,, Mohd Farihan Md Yatim,, Aishah Hani Azil,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/18345/1/3.pdf
http://journalarticle.ukm.my/18345/
https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
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spelling my-ukm.journal.183452022-04-11T06:11:32Z http://journalarticle.ukm.my/18345/ Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia Noor Shazleen Husnie Mohd Mohtar, Emelia Osman, Mohd Farihan Md Yatim, Aishah Hani Azil, Wolbachia has the ability to cause reproductive abnormalities in infected hosts including cytoplasmic incompatibility (CI). CI is activated when there are multiple Wolbachia supergroups or strains infection present in insect populations. Wolbachia-transinfected mosquitoes have been used widely in some countries as a biological control agent. In order to ensure a successful Wolbachia establishment, it is important to determine the diversity of natural Wolbachia present in the wild mosquito populations. The adults and immature stages of mosquitoes were collected from urban, suburban and rural areas and were reared into adults and identified to species before being subjected to molecular analysis. We found that 22% out of 222 males and 34.6% of 543 females tested were carrying Wolbachia based on PCR amplification of the Wolbachia 16S rDNA genes technique. PCR digestion for Wolbachia supergrouping showed that most of the Ae. albopictus were superinfected with Wolbachia (52.41%), whereas 21% and 28% of the positive samples were singly infected with supergroup A and B, respectively. There is an indication that prevalence of Wolbachia varies between mosquito populations in different areas. However, further studies to incorporate both PCR amplication of the Wolbachia 16S rDNA and wsp genes with bigger sample size should be performed to measure exact infection of Wolbachia in Malaysia. The baseline data on diversity of Wolbachia supergroups is expected to facilitate Wolbachia strategy by helping us to better understand the patterns and impact of the bacteria’s transmission in the environment. Penerbit Universiti Kebangsaan Malaysia 2022-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18345/1/3.pdf Noor Shazleen Husnie Mohd Mohtar, and Emelia Osman, and Mohd Farihan Md Yatim, and Aishah Hani Azil, (2022) Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia. Sains Malaysiana, 51 (1). pp. 27-38. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Wolbachia has the ability to cause reproductive abnormalities in infected hosts including cytoplasmic incompatibility (CI). CI is activated when there are multiple Wolbachia supergroups or strains infection present in insect populations. Wolbachia-transinfected mosquitoes have been used widely in some countries as a biological control agent. In order to ensure a successful Wolbachia establishment, it is important to determine the diversity of natural Wolbachia present in the wild mosquito populations. The adults and immature stages of mosquitoes were collected from urban, suburban and rural areas and were reared into adults and identified to species before being subjected to molecular analysis. We found that 22% out of 222 males and 34.6% of 543 females tested were carrying Wolbachia based on PCR amplification of the Wolbachia 16S rDNA genes technique. PCR digestion for Wolbachia supergrouping showed that most of the Ae. albopictus were superinfected with Wolbachia (52.41%), whereas 21% and 28% of the positive samples were singly infected with supergroup A and B, respectively. There is an indication that prevalence of Wolbachia varies between mosquito populations in different areas. However, further studies to incorporate both PCR amplication of the Wolbachia 16S rDNA and wsp genes with bigger sample size should be performed to measure exact infection of Wolbachia in Malaysia. The baseline data on diversity of Wolbachia supergroups is expected to facilitate Wolbachia strategy by helping us to better understand the patterns and impact of the bacteria’s transmission in the environment.
format Article
author Noor Shazleen Husnie Mohd Mohtar,
Emelia Osman,
Mohd Farihan Md Yatim,
Aishah Hani Azil,
spellingShingle Noor Shazleen Husnie Mohd Mohtar,
Emelia Osman,
Mohd Farihan Md Yatim,
Aishah Hani Azil,
Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
author_facet Noor Shazleen Husnie Mohd Mohtar,
Emelia Osman,
Mohd Farihan Md Yatim,
Aishah Hani Azil,
author_sort Noor Shazleen Husnie Mohd Mohtar,
title Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
title_short Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
title_full Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
title_fullStr Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
title_full_unstemmed Super- or single infection : Wolbachia supergrouping of wild mosquito populations from varied location types in Peninsular Malaysia
title_sort super- or single infection : wolbachia supergrouping of wild mosquito populations from varied location types in peninsular malaysia
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/18345/1/3.pdf
http://journalarticle.ukm.my/18345/
https://www.ukm.my/jsm/malay_journals/jilid51bil1_2022/KandunganJilid51Bil1_2022.html
_version_ 1731226524193914880
score 13.211869

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