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Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model

Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The inv...

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Main Authors: Pissared Khuituan,, Chotika Nhaemchei,, Sakda Pradab,, Sakena K-da,, Nipaporn Konthapakdee,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18332/1/15.pdf
http://journalarticle.ukm.my/18332/
https://www.ukm.my/jsm/malay_journals/jilid50bil12_2021/KandunganJilid50Bil12_2021.html
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spelling my-ukm.journal.183322022-04-11T04:54:45Z http://journalarticle.ukm.my/18332/ Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model Pissared Khuituan, Chotika Nhaemchei, Sakda Pradab, Sakena K-da, Nipaporn Konthapakdee, Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The investigation aimed to determine related mechanisms using an isolated mouse intestine model. Our study showed that fluoxetine at 0.001 μM increased the amplitude of contraction in colonic tissue but decreased the amplitude in duodenal tissue. The direct application of higher concentrations of fluoxetine (1, 10, and 100 μM) reduced the amplitude of contractions in proximal colonic tissue. Moreover, we found that the stimulatory effect of 0.001 μM fluoxetine on the tone of contractions could be prevented by pre-incubating the tissue in ondansetron and atropine. Our findings suggest that the inhibition of the effect of fluoxetine was mainly mediated via 5-HT3 receptors and muscarinic signaling. These findings might explain the conflicting gastrointestinal symptoms caused by fluoxetine. Penerbit Universiti Kebangsaan Malaysia 2021-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18332/1/15.pdf Pissared Khuituan, and Chotika Nhaemchei, and Sakda Pradab, and Sakena K-da, and Nipaporn Konthapakdee, (2021) Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model. Sains Malaysiana, 50 (12). pp. 3647-3657. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil12_2021/KandunganJilid50Bil12_2021.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Fluoxetine, a selective serotonin reuptake inhibitor anti-depressant, causes undesirable side effects, including diarrhea and constipation. This research investigated the direct effects of fluoxetine at 0.001, 0.01, 0.1, 1, 10, and 100 μM on duodenal and proximal colonic tissue contractions. The investigation aimed to determine related mechanisms using an isolated mouse intestine model. Our study showed that fluoxetine at 0.001 μM increased the amplitude of contraction in colonic tissue but decreased the amplitude in duodenal tissue. The direct application of higher concentrations of fluoxetine (1, 10, and 100 μM) reduced the amplitude of contractions in proximal colonic tissue. Moreover, we found that the stimulatory effect of 0.001 μM fluoxetine on the tone of contractions could be prevented by pre-incubating the tissue in ondansetron and atropine. Our findings suggest that the inhibition of the effect of fluoxetine was mainly mediated via 5-HT3 receptors and muscarinic signaling. These findings might explain the conflicting gastrointestinal symptoms caused by fluoxetine.
format Article
author Pissared Khuituan,
Chotika Nhaemchei,
Sakda Pradab,
Sakena K-da,
Nipaporn Konthapakdee,
spellingShingle Pissared Khuituan,
Chotika Nhaemchei,
Sakda Pradab,
Sakena K-da,
Nipaporn Konthapakdee,
Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
author_facet Pissared Khuituan,
Chotika Nhaemchei,
Sakda Pradab,
Sakena K-da,
Nipaporn Konthapakdee,
author_sort Pissared Khuituan,
title Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
title_short Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
title_full Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
title_fullStr Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
title_full_unstemmed Fluoxetine affects intestinal motility via 5-HT3 and muscarinic receptors in ex vivo mouse model
title_sort fluoxetine affects intestinal motility via 5-ht3 and muscarinic receptors in ex vivo mouse model
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/18332/1/15.pdf
http://journalarticle.ukm.my/18332/
https://www.ukm.my/jsm/malay_journals/jilid50bil12_2021/KandunganJilid50Bil12_2021.html
_version_ 1731226522323255296
score 13.211869

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