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Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography

Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and...

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Main Authors: Roza Linda,, Abdullah,, Mohamad Rafi,, Aster Rahayu,, Lee, Lee Wah, Takeuchi, Toyohide
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Online Access:http://journalarticle.ukm.my/18296/1/8.pdf
http://journalarticle.ukm.my/18296/
https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html
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spelling my-ukm.journal.182962022-04-06T06:09:46Z http://journalarticle.ukm.my/18296/ Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography Roza Linda, Abdullah, Mohamad Rafi, Aster Rahayu, Lee, Lee Wah Takeuchi, Toyohide Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and 60 Å mean pore diameter). The prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol, pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole and/or hydrogen-bonding interactions. Penerbit Universiti Kebangsaan Malaysia 2021-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/18296/1/8.pdf Roza Linda, and Abdullah, and Mohamad Rafi, and Aster Rahayu, and Lee, Lee Wah and Takeuchi, Toyohide (2021) Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography. Sains Malaysiana, 50 (11). pp. 3241-3250. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Poly(ethylene oxide) (PEO) bonded stationary phase has been synthesized by a single and simple step reaction. Poly(ethylene glycol monomethyl ether p-toluene sulfonate) (tosylated-PEO, molecular weight 900, n ≈ 18) was chemically bonded to 3-aminopropyl silica (TSKgel NH2-60, 5 μm particle size, and 60 Å mean pore diameter). The prepared stationary phase was able to separate polar compounds such as phenolics and nucleobases in capillary liquid chromatography. The retention and separation of phenolics and nucleobases could be achieved under isocratic elution condition. Nucleobases such as thymine, adenine, uracil, uridine, cytidine and toluene and phenolics (phenol, pyrocatechol, pyrogallol) were baseline separated in less than 6 min using 98% acetonitrile and less than 7 minutes using 80% acetonitrile, respectively. We demonstrated that the retention of nucleobases as analyte decreased with decreasing eluent concentration. The retention of these polar compounds was believed to be based on dipole-dipole and/or hydrogen-bonding interactions.
format Article
author Roza Linda,
Abdullah,
Mohamad Rafi,
Aster Rahayu,
Lee, Lee Wah
Takeuchi, Toyohide
spellingShingle Roza Linda,
Abdullah,
Mohamad Rafi,
Aster Rahayu,
Lee, Lee Wah
Takeuchi, Toyohide
Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
author_facet Roza Linda,
Abdullah,
Mohamad Rafi,
Aster Rahayu,
Lee, Lee Wah
Takeuchi, Toyohide
author_sort Roza Linda,
title Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
title_short Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
title_full Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
title_fullStr Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
title_full_unstemmed Separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
title_sort separation of polar compounds using poly(ethylene oxide) bonded stationary capillary liquid chromatography
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://journalarticle.ukm.my/18296/1/8.pdf
http://journalarticle.ukm.my/18296/
https://www.ukm.my/jsm/malay_journals/jilid50bil11_2021/KandunganJilid50Bil11_2021.html
_version_ 1729704087752015872
score 13.211869

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