Polyphenolic profile and antioxidant activities of freeze-dried Melon Manis Terengganu peel extracts
Melon Manis Terengganu (MMT) also known as Cucumis melo var. Inodorus cv. Manis Terengganu 1 originates from Terengganu, Malaysia, is composed of 28–30% inedible peels and discarded as waste. Hence, this study aimed to quantify the polyphenol and flavonoid contents, identify polyphenolic compounds...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17905/1/181-188%2B%2B%2BNorshazila%2BShahidan.pdf http://journalarticle.ukm.my/17905/ https://jms.mabjournal.com/index.php/mab/issue/view/2 |
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| Summary: | Melon Manis Terengganu (MMT) also known as Cucumis melo var. Inodorus cv. Manis Terengganu 1 originates from
Terengganu, Malaysia, is composed of 28–30% inedible peels and discarded as waste. Hence, this study aimed to quantify
the polyphenol and flavonoid contents, identify polyphenolic compounds and evaluate the antioxidant activity of freezedried
Melon Manis Terengganu (MMT) peel aqueous extract. The total phenolic and flavonoid contents were determined
spectrophotometrically by gallic acid and quercetin standard curves, respectively. Whereas, antioxidant activity was explored
by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylben-zothiazoline-6-sulfonic acid) diammonium
salt (ABTS) assay. Next, liquid chromatography-mass spectrometry (LC-MS) was applied for phenolic compounds
identification. Results reported that the total phenolic content was 12.35 (0.59) μg GAE/mg while total flavonoid content
was 2.01 (0.70) μg QE/mg. The IC50 of DPPH and ABTS assay were 27.74 (1.59) mg/mL and 4.87 (0.06) mg/mL,
respectively. LC-MS results revealed the presence of polyphenolic compounds, such as kaempferol 3-(6'’-sinapylglucosyl)-
(1->2)-galactoside), isoorientin 7-O-(6'”-O-(E)-feruloyl)glucoside and isoscoparin 2'’-(6-(E)-ferulylglucoside) in the sample
extract. In summary, these findings served as preliminary data for further exploration on the potential application of freezedried
MMT peel aqueous extract in the food and nutraceutical industry. |
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