The establishment of aseptic cultures and multiple shoot induction of olive (Olea europaea) cv.1
Olea europaea or the olive plant is from the family of Oleaceae and has been cultivated for its fruits that are highly nutritious, capable of reducing risks of cardiovascular diseases and to prevent cancer. The current propagation technique of olive plants in Malaysia relies on conventional method...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2021
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| Online Access: | http://journalarticle.ukm.my/17899/1/125-132%2B%2B%2BBee%2BLynn%2BChew.pdf http://journalarticle.ukm.my/17899/ https://jms.mabjournal.com/index.php/mab/issue/view/2 |
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| Summary: | Olea europaea or the olive plant is from the family of Oleaceae and has been cultivated for its fruits that are highly
nutritious, capable of reducing risks of cardiovascular diseases and to prevent cancer. The current propagation technique of
olive plants in Malaysia relies on conventional methods that are less efficient in producing good quality plant stocks. Plant
tissue culture offers an alternative to multiply plants from novel mother plants and is commonly applied in the propagation
of various crops. This study aims to establish sterile cultures and to induce multiple shoots from Olea europaea cv.1 as a
preliminary study to micropropagate olive plants for commercial farms in Malaysia. Nodal explants were surfaced sterilised
with ethanol and Clorox© at different durations followed by treatments in MS media supplemented with different
concentrations of Zeatin and BAP. Nodal explants surface sterilised with 70% (v/v) ethanol for 2 min and 30% (v/v)
Clorox© for 8 min produced sterile explants with the survival rate of 85%. MS media supplemented 5.0 mg/L BAP was
optimal for shoot induction (2.10 ± 0.31 shoots per explant) and shoot elongation (6.50 ± 1.17 mm). The current study
serves as preliminary assessment for the establishment of local olive cultures. |
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