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Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract

Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes. The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics analysis. However, their non-enzymatic oxidative re...

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Main Authors: Khalida Khalil,, Syarul Nataqain Baharum,, Shazrul Fazry,, Nik Marzuki Sidik,, Fareed Sairi,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/17390/1/49_05_12.pdf
http://journalarticle.ukm.my/17390/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.173902021-08-30T03:59:06Z http://journalarticle.ukm.my/17390/ Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract Khalida Khalil, Syarul Nataqain Baharum, Shazrul Fazry, Nik Marzuki Sidik, Fareed Sairi, Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes. The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics analysis. However, their non-enzymatic oxidative responses have yet to be elucidated. Therefore, this study aims to characterize the antioxidant activity and identify the non-enzymatic antioxidant compounds from Pomacea maculata. To address the aims, a polar and non-polar extraction of snail-whole body extract was conducted using methanol and chloroform, respectively. The antioxidant activity of both extracts was elucidated by Folin Ciocalteau (FC), DPPH, and reducing power assay. LC-MS/MS was then used to profile both extracts. The results demonstrated that the crude methanol extract (CME) contains a higher antioxidant capacity (FC=43.22 ± 3.02 mg GAE/ g extract, DPPH IC50=0.073 mg/mL, and reducing the power of methanol and chloroform extract are 0.361 ± 0.07 and 0.051 ± 0.003 respectively). Profiling of the snail metabolites by LC-MS/MS from both extracts resulted in the identification of uric acid and phenolic compounds . The former was detected at the highest intensity in CME followed by crude chloroform extract (CCE). The phenolic compounds, however, were hypothetically identified as plant metabolites. Therefore, the study suggested that antioxidant activity exhibited by P. maculata extracts were due to non-enzymatic compounds such as uric acid and phenolic compounds originated from the animal’s metabolic activity and plants, respectively. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17390/1/49_05_12.pdf Khalida Khalil, and Syarul Nataqain Baharum, and Shazrul Fazry, and Nik Marzuki Sidik, and Fareed Sairi, (2020) Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract. Malaysian Applied Biology, 49 (5). pp. 115-124. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Pomacea sp. is a freshwater gastropod that is capable of withstanding oxidative stress during extreme environmental changes. The snail enzymatic oxidative responses have already been elucidated through biochemical, transcriptomics, and proteomics analysis. However, their non-enzymatic oxidative responses have yet to be elucidated. Therefore, this study aims to characterize the antioxidant activity and identify the non-enzymatic antioxidant compounds from Pomacea maculata. To address the aims, a polar and non-polar extraction of snail-whole body extract was conducted using methanol and chloroform, respectively. The antioxidant activity of both extracts was elucidated by Folin Ciocalteau (FC), DPPH, and reducing power assay. LC-MS/MS was then used to profile both extracts. The results demonstrated that the crude methanol extract (CME) contains a higher antioxidant capacity (FC=43.22 ± 3.02 mg GAE/ g extract, DPPH IC50=0.073 mg/mL, and reducing the power of methanol and chloroform extract are 0.361 ± 0.07 and 0.051 ± 0.003 respectively). Profiling of the snail metabolites by LC-MS/MS from both extracts resulted in the identification of uric acid and phenolic compounds . The former was detected at the highest intensity in CME followed by crude chloroform extract (CCE). The phenolic compounds, however, were hypothetically identified as plant metabolites. Therefore, the study suggested that antioxidant activity exhibited by P. maculata extracts were due to non-enzymatic compounds such as uric acid and phenolic compounds originated from the animal’s metabolic activity and plants, respectively.
format Article
author Khalida Khalil,
Syarul Nataqain Baharum,
Shazrul Fazry,
Nik Marzuki Sidik,
Fareed Sairi,
spellingShingle Khalida Khalil,
Syarul Nataqain Baharum,
Shazrul Fazry,
Nik Marzuki Sidik,
Fareed Sairi,
Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
author_facet Khalida Khalil,
Syarul Nataqain Baharum,
Shazrul Fazry,
Nik Marzuki Sidik,
Fareed Sairi,
author_sort Khalida Khalil,
title Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
title_short Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
title_full Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
title_fullStr Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
title_full_unstemmed Non-enzymatic antioxidant from apple snail (Pomacea maculata) extract
title_sort non-enzymatic antioxidant from apple snail (pomacea maculata) extract
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/17390/1/49_05_12.pdf
http://journalarticle.ukm.my/17390/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1078&catid=59:current-view&Itemid=56
_version_ 1709668484510646272
score 13.211869

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