Commercial eye drops triggers necrotic effect in Acanthamoeba sp.
Eye infection due to microbial infection is hard to treat and painful. Two types of eye drops that are commonly used to relieve eye pain offered by pharmacists have been tested in this study namely solution A (containing tetrahydrozoline hydrochloride) and solution B (containing gentamicin and dex...
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Penerbit Universiti Kebangsaan Malaysia
2020
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my-ukm.journal.172262021-07-29T03:04:50Z http://journalarticle.ukm.my/17226/ Commercial eye drops triggers necrotic effect in Acanthamoeba sp. Fatimah Hashim, Nur A’fiefah Mohd Zulkeffli, Zafirah Najwa Zainal Abidin, Muhamad Fairus Noor Hassim, Ramesh Kumar Santhanam, Eye infection due to microbial infection is hard to treat and painful. Two types of eye drops that are commonly used to relieve eye pain offered by pharmacists have been tested in this study namely solution A (containing tetrahydrozoline hydrochloride) and solution B (containing gentamicin and dexamethasone). The efficacy and the activities of these eye drops were tested on Acanthamoeba sp. (a clinical isolate from an Acanthamoeba keratitis patient) to study the cytotoxicity effects of the solutions on the Acanthamoeba. The Acanthamoeba were exposed to solution A and B for 24 hr and cell viability was assessed using MTT assays, morphological changes using the light microscope and through acridine orange and propidium iodide (AO/PI) staining for cytoplasmic biochemical activities. The IC50 value for Acanthamoeba cell viability was 45.1% and 20.3% for solutions A and B respectively. Morphological observation shows the inhibition of acanthapodia formation on the surface of the cells. Solution A and B-treated Acanthamoeba appeared in the red color of the cytoplasm upon staining with AO/PI indicating a necrotic mode of cell death. This is due to loss of membrane integrity of Acanthamoeba cell membrane after exposed to solution A and B at their IC50 value. It is shown that solutions A and B can cause cell death in trophozoite of Acanthamoeba cells at moderate IC50 value. Unfortunately, the necrosis mode of cell death is not a preferable type of cell death for treating Acanthamoeba infection. Therefore, it can be concluded that solutions A and B are not suitable to treat eye infected with Acanthamoeba sp. as it does not promise absolute healing as the solution concentration needed is quite high and the cell death mechanism is necrosis. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17226/1/49_04_11.pdf Fatimah Hashim, and Nur A’fiefah Mohd Zulkeffli, and Zafirah Najwa Zainal Abidin, and Muhamad Fairus Noor Hassim, and Ramesh Kumar Santhanam, (2020) Commercial eye drops triggers necrotic effect in Acanthamoeba sp. Malaysian Applied Biology, 49 (4). pp. 85-90. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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Eye infection due to microbial infection is hard to treat and painful. Two types of eye drops that are commonly used to
relieve eye pain offered by pharmacists have been tested in this study namely solution A (containing tetrahydrozoline
hydrochloride) and solution B (containing gentamicin and dexamethasone). The efficacy and the activities of these eye drops
were tested on Acanthamoeba sp. (a clinical isolate from an Acanthamoeba keratitis patient) to study the cytotoxicity effects
of the solutions on the Acanthamoeba. The Acanthamoeba were exposed to solution A and B for 24 hr and cell viability was
assessed using MTT assays, morphological changes using the light microscope and through acridine orange and propidium
iodide (AO/PI) staining for cytoplasmic biochemical activities. The IC50 value for Acanthamoeba cell viability was 45.1%
and 20.3% for solutions A and B respectively. Morphological observation shows the inhibition of acanthapodia formation on
the surface of the cells. Solution A and B-treated Acanthamoeba appeared in the red color of the cytoplasm upon staining
with AO/PI indicating a necrotic mode of cell death. This is due to loss of membrane integrity of Acanthamoeba cell membrane
after exposed to solution A and B at their IC50 value. It is shown that solutions A and B can cause cell death in trophozoite of
Acanthamoeba cells at moderate IC50 value. Unfortunately, the necrosis mode of cell death is not a preferable type of cell
death for treating Acanthamoeba infection. Therefore, it can be concluded that solutions A and B are not suitable to treat eye
infected with Acanthamoeba sp. as it does not promise absolute healing as the solution concentration needed is quite high
and the cell death mechanism is necrosis. |
| format |
Article |
| author |
Fatimah Hashim, Nur A’fiefah Mohd Zulkeffli, Zafirah Najwa Zainal Abidin, Muhamad Fairus Noor Hassim, Ramesh Kumar Santhanam, |
| spellingShingle |
Fatimah Hashim, Nur A’fiefah Mohd Zulkeffli, Zafirah Najwa Zainal Abidin, Muhamad Fairus Noor Hassim, Ramesh Kumar Santhanam, Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| author_facet |
Fatimah Hashim, Nur A’fiefah Mohd Zulkeffli, Zafirah Najwa Zainal Abidin, Muhamad Fairus Noor Hassim, Ramesh Kumar Santhanam, |
| author_sort |
Fatimah Hashim, |
| title |
Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| title_short |
Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| title_full |
Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| title_fullStr |
Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| title_full_unstemmed |
Commercial eye drops triggers necrotic effect in Acanthamoeba sp. |
| title_sort |
commercial eye drops triggers necrotic effect in acanthamoeba sp. |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2020 |
| url |
http://journalarticle.ukm.my/17226/1/49_04_11.pdf http://journalarticle.ukm.my/17226/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
| _version_ |
1706958281807757312 |
| score |
13.211869 |