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Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11

The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In t...

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Main Authors: Jun, Meng Chai, Shaliny Hari Rama Krishnan,, Tan, Vincent Yee Hang, Hanis Athirah Mohd Hamdan,, Nurul Nadhirah Ruzelan,, Sevakumaran Vigneswari,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/17225/1/49_04_10.pdf
http://journalarticle.ukm.my/17225/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.172252021-07-29T03:03:26Z http://journalarticle.ukm.my/17225/ Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11 Jun, Meng Chai Shaliny Hari Rama Krishnan, Tan, Vincent Yee Hang Hanis Athirah Mohd Hamdan, Nurul Nadhirah Ruzelan, Sevakumaran Vigneswari, The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The carbon precursors 1,6-hexandiol, 1,4-butanediol, and γ-butyrolactone were used to screen for high cell biomass, PHA content, and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors showed an increase in cell biomass. The carbon combination of 1,4-butanediol and γ-butyrolactone at the ratio of 4:2 and carbon combination of 1,6-hexanediol and γ-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35 g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1 mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of 1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6 mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17225/1/49_04_10.pdf Jun, Meng Chai and Shaliny Hari Rama Krishnan, and Tan, Vincent Yee Hang and Hanis Athirah Mohd Hamdan, and Nurul Nadhirah Ruzelan, and Sevakumaran Vigneswari, (2020) Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11. Malaysian Applied Biology, 49 (4). pp. 79-84. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The P(3HB-co-4HB) is commonly used for biomedical applications. This is due to the desired mechanical properties as well as biocompatibility, non-genotoxicity, and non-cytotoxicity of this copolymer. However, the production of P(3HB-co-4HB) with a specific 4HB molar fraction is still limited. In this study, carbon precursors at different combinations and concentration ratios have been used in the production of P(3HB-co-4HB) bioplastics using locally isolated Cupriavidus sp. TMT11. The carbon precursors 1,6-hexandiol, 1,4-butanediol, and γ-butyrolactone were used to screen for high cell biomass, PHA content, and 4HB molar fraction through Gas Chromatography (GC) analysis. Generally, the combination of various carbon precursors showed an increase in cell biomass. The carbon combination of 1,4-butanediol and γ-butyrolactone at the ratio of 4:2 and carbon combination of 1,6-hexanediol and γ-butyrolactone at a ratio of 1:5 showed high amount of cell biomass above 0.35 g/L on day 3. Nevertheless, the 4HB molar fraction of both the combination was recorded as 9±0.27 mol% and 14±1.1 mol% respectively. The lowest amount of cell biomass and PHA yield were recorded with the carbon combination of 1,6-hexanediol and 1,4-butanediol with the ratio 5:1 at 0.55±0.13 g/L. However, the highest 4HB molar fraction of 89.37±3.6 mol% 4HB was recorded with this combination. The 4HB molar fraction above 80 mol% was reported with a carbon combination of 1,6-hexanediol +1,4-butanediol at 5:1, 1:1, and 2:4 ratio. The varying combination of carbon precursors biosynthesized a wide range of 4HB molar fractions ranging from 9.07 to 89.37 mol% 4HB.
format Article
author Jun, Meng Chai
Shaliny Hari Rama Krishnan,
Tan, Vincent Yee Hang
Hanis Athirah Mohd Hamdan,
Nurul Nadhirah Ruzelan,
Sevakumaran Vigneswari,
spellingShingle Jun, Meng Chai
Shaliny Hari Rama Krishnan,
Tan, Vincent Yee Hang
Hanis Athirah Mohd Hamdan,
Nurul Nadhirah Ruzelan,
Sevakumaran Vigneswari,
Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
author_facet Jun, Meng Chai
Shaliny Hari Rama Krishnan,
Tan, Vincent Yee Hang
Hanis Athirah Mohd Hamdan,
Nurul Nadhirah Ruzelan,
Sevakumaran Vigneswari,
author_sort Jun, Meng Chai
title Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
title_short Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
title_full Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
title_fullStr Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
title_full_unstemmed Effects of various carbon precursors combination in regulating the molar fraction of P(3HB-co-4HB) using locally isolated Cupriavidus sp. TMT11
title_sort effects of various carbon precursors combination in regulating the molar fraction of p(3hb-co-4hb) using locally isolated cupriavidus sp. tmt11
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/17225/1/49_04_10.pdf
http://journalarticle.ukm.my/17225/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
_version_ 1706958281660956672
score 13.211869

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