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Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+

Heavy metals accumulation in aquatic bodies will lead to many deleterious effects on organisms living in them. Furthermore, some organisms can accumulate metals and this will eventually reach the primary consumers. The application of microalgae as remediator and indicator of metals pollution has b...

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Main Authors: Hazlina Ahamad Zakeri,, Siti Zulaikha Razak,, Hanis Hazwani Hasmudin,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/17223/1/49_04_08.pdf
http://journalarticle.ukm.my/17223/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.172232021-07-29T03:00:46Z http://journalarticle.ukm.my/17223/ Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+ Hazlina Ahamad Zakeri, Siti Zulaikha Razak, Hanis Hazwani Hasmudin, Heavy metals accumulation in aquatic bodies will lead to many deleterious effects on organisms living in them. Furthermore, some organisms can accumulate metals and this will eventually reach the primary consumers. The application of microalgae as remediator and indicator of metals pollution has become an increasing interest to scientists. In this study, we reported on the defence responses of a marine microalga, Chlorella vulgaris (UMT-M1) against Pb2+ and Hg2+ in terms of three antioxidative enzymes activity: superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The activity of the enzymes was measured in alga treated with 5.7 mg/L PbCl2 and 0.18 mg/L Hg(NO3)2. These two concentrations were the IC50 values calculated using probit analysis. It was observed that Pb2+ and Hg2+ triggered a different strategy in counterattacking the metal’s effects. While a significant induction of SOD and CAT activity was evident in alga treated with both metals, APX activity was significantly inhibited in Pb2+-treated alga but was stimulated by more than 4-fold from the control in Hg2+-treated alga. Higher SOD activity was observed in Pb2+- compared to Hg2+-treated alga. However, lower CAT activity was observed in Pb2+- compared to Hg2+-treated alga. Also, a significant reduction in total soluble protein (TSP) content was observed in alga treated with Hg2+. Pb2+, on the other hand, significantly increased the production of TSP. The changes observed in the antioxidative enzymes activity and TSP content in this alga are good potentials to be used as biomarkers for metal’s bioindication tool. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17223/1/49_04_08.pdf Hazlina Ahamad Zakeri, and Siti Zulaikha Razak, and Hanis Hazwani Hasmudin, (2020) Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+. Malaysian Applied Biology, 49 (4). pp. 63-70. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Heavy metals accumulation in aquatic bodies will lead to many deleterious effects on organisms living in them. Furthermore, some organisms can accumulate metals and this will eventually reach the primary consumers. The application of microalgae as remediator and indicator of metals pollution has become an increasing interest to scientists. In this study, we reported on the defence responses of a marine microalga, Chlorella vulgaris (UMT-M1) against Pb2+ and Hg2+ in terms of three antioxidative enzymes activity: superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). The activity of the enzymes was measured in alga treated with 5.7 mg/L PbCl2 and 0.18 mg/L Hg(NO3)2. These two concentrations were the IC50 values calculated using probit analysis. It was observed that Pb2+ and Hg2+ triggered a different strategy in counterattacking the metal’s effects. While a significant induction of SOD and CAT activity was evident in alga treated with both metals, APX activity was significantly inhibited in Pb2+-treated alga but was stimulated by more than 4-fold from the control in Hg2+-treated alga. Higher SOD activity was observed in Pb2+- compared to Hg2+-treated alga. However, lower CAT activity was observed in Pb2+- compared to Hg2+-treated alga. Also, a significant reduction in total soluble protein (TSP) content was observed in alga treated with Hg2+. Pb2+, on the other hand, significantly increased the production of TSP. The changes observed in the antioxidative enzymes activity and TSP content in this alga are good potentials to be used as biomarkers for metal’s bioindication tool.
format Article
author Hazlina Ahamad Zakeri,
Siti Zulaikha Razak,
Hanis Hazwani Hasmudin,
spellingShingle Hazlina Ahamad Zakeri,
Siti Zulaikha Razak,
Hanis Hazwani Hasmudin,
Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
author_facet Hazlina Ahamad Zakeri,
Siti Zulaikha Razak,
Hanis Hazwani Hasmudin,
author_sort Hazlina Ahamad Zakeri,
title Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
title_short Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
title_full Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
title_fullStr Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
title_full_unstemmed Antioxidative enzymes activity of Chlorella vulgaris (UMT-M1) in response to IC50 of Pb2+ and Hg2+
title_sort antioxidative enzymes activity of chlorella vulgaris (umt-m1) in response to ic50 of pb2+ and hg2+
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/17223/1/49_04_08.pdf
http://journalarticle.ukm.my/17223/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56
_version_ 1706958281392521216
score 13.211869

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