Cover Image

Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant

Banana blood disease (BBD) caused by blood disease bacterium (BDB) is one of the major diseases of Indonesian banana plants. The foraging activity of flower-visiting insects was suspected as the vectors of the disease. This study aims at analyzing the potential of the sap-feeding beetle, Nitidulid...

Full description

Saved in:
Bibliographic Details
Main Authors: Tjandra Anggraeni,, Masriany,, M. Dwivany, Fenny, Rizkita R. Esyanti,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/16017/1/49_02_01.pdf
http://journalarticle.ukm.my/16017/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.16017
record_format eprints
spelling my-ukm.journal.160172020-12-18T02:06:13Z http://journalarticle.ukm.my/16017/ Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant Tjandra Anggraeni, Masriany, M. Dwivany, Fenny Rizkita R. Esyanti, Banana blood disease (BBD) caused by blood disease bacterium (BDB) is one of the major diseases of Indonesian banana plants. The foraging activity of flower-visiting insects was suspected as the vectors of the disease. This study aims at analyzing the potential of the sap-feeding beetle, Nitidulidae, as a vector of BDB. Beetle collection was done by using purposive sampling method on three cultivars of banana inflorescence: Nangka, Klutuk, and Siem. The bacterium was isolated from the outside and the inside of beetle tissues and was followed with bacterial DNA extraction. Confirmation of bacterial cell culture as BDB was conducted by biochemical assay and PCR using 16S rDNA oligonucleotide. The findings revealed that the beetle population in the three cultivars of banana cultivars was Nangka (66%), Siem (19%) and Klutuk (16%). According to the biochemical assay and PCR result, it has been found that the bacterial cell culture was found positive as BDB, which was putative 79% and homolog with BDB that is in accordance with the data provided by NCBI. Thereby, the discovery of BDB in sap-feeding beetle, Nitidulidae, showed that the foraging activity has the potential as an agent of spreader of BDB in banana plants. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/16017/1/49_02_01.pdf Tjandra Anggraeni, and Masriany, and M. Dwivany, Fenny and Rizkita R. Esyanti, (2020) Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant. Malaysian Applied Biology, 49 (2). pp. 1-5. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Banana blood disease (BBD) caused by blood disease bacterium (BDB) is one of the major diseases of Indonesian banana plants. The foraging activity of flower-visiting insects was suspected as the vectors of the disease. This study aims at analyzing the potential of the sap-feeding beetle, Nitidulidae, as a vector of BDB. Beetle collection was done by using purposive sampling method on three cultivars of banana inflorescence: Nangka, Klutuk, and Siem. The bacterium was isolated from the outside and the inside of beetle tissues and was followed with bacterial DNA extraction. Confirmation of bacterial cell culture as BDB was conducted by biochemical assay and PCR using 16S rDNA oligonucleotide. The findings revealed that the beetle population in the three cultivars of banana cultivars was Nangka (66%), Siem (19%) and Klutuk (16%). According to the biochemical assay and PCR result, it has been found that the bacterial cell culture was found positive as BDB, which was putative 79% and homolog with BDB that is in accordance with the data provided by NCBI. Thereby, the discovery of BDB in sap-feeding beetle, Nitidulidae, showed that the foraging activity has the potential as an agent of spreader of BDB in banana plants.
format Article
author Tjandra Anggraeni,
Masriany,
M. Dwivany, Fenny
Rizkita R. Esyanti,
spellingShingle Tjandra Anggraeni,
Masriany,
M. Dwivany, Fenny
Rizkita R. Esyanti,
Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
author_facet Tjandra Anggraeni,
Masriany,
M. Dwivany, Fenny
Rizkita R. Esyanti,
author_sort Tjandra Anggraeni,
title Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
title_short Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
title_full Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
title_fullStr Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
title_full_unstemmed Possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
title_sort possibility of sap-feeding beetle, nitidulidae, as a spreading agent for blood disease bacterium on the banana plant
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/16017/1/49_02_01.pdf
http://journalarticle.ukm.my/16017/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=1003&catid=59:current-view&Itemid=56
_version_ 1687394683412545536
score 13.211869

Similar Items