Cover Image

Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves

Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant...

Full description

Saved in:
Bibliographic Details
Main Authors: Farazimah Yakop,, Malai Haniti Sheikh Abdul Hamid,, Norhayati Ahmad,, Mohamed Abdul Majid,, Manoharan Karuppiah Pillai,, Hussein Taha,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/15707/1/49_01_14.pdf
http://journalarticle.ukm.my/15707/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.15707
record_format eprints
spelling my-ukm.journal.157072020-11-18T06:02:19Z http://journalarticle.ukm.my/15707/ Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves Farazimah Yakop, Malai Haniti Sheikh Abdul Hamid, Norhayati Ahmad, Mohamed Abdul Majid, Manoharan Karuppiah Pillai, Hussein Taha, Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant and antibacterial activities of aqueous extract, methanol extract and its fractions obtained from the leaves of D. suffruticosa. The dried leaves were extracted using methanol before successive solvent partitioning was carried out on the extract using hexane, chloroform, ethyl acetate and diethyl ether. In addition to this, aqueous decoction was conducted. The antioxidant activities were determined using Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging methods. Phytochemical screening had shown that most of the extracts and fractions contained alkaloids, steroids, phenolics, flavonoids and saponins. The diethyl ether and ethyl acetate fractions showed higher TPC and TFC values. The diethyl ether and ethyl acetate fractions also showed higher antioxidant activities determined via DPPH assay. Antibacterial activities determined using disc diffusion assay showed the methanol extract and its fractions had antibacterial activity against Staphylococcus aureus, with the diethyl ether fraction having comparable activity with the standard antibiotic streptomycin. However, inhibition against Bacillus subtilis was only observed in hexane, chloroform and diethyl ether fractions. No inhibition was observed against Escherichia coli and Pseudomonas aeruginosa. This study identified the diethyl ethyl and ethyl acetate fractions of D. suffruticosa leaves as potential sources of bioactive compounds. Further investigations on the bioassay-guided isolation of bioactive compounds of this species may lead to the discovery of new pharmaceutical or effective antimicrobial agents. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15707/1/49_01_14.pdf Farazimah Yakop, and Malai Haniti Sheikh Abdul Hamid, and Norhayati Ahmad, and Mohamed Abdul Majid, and Manoharan Karuppiah Pillai, and Hussein Taha, (2020) Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves. Malaysian Applied Biology, 49 (1). pp. 121-130. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Dillenia suffruticosa or ‘Simpur bini’ is known to have ethnomedicinal properties and had been used traditionally to heal wounds, relieve fever and rheumatism. There has been limited studies carried out on this species, therefore, this study aims to evaluate the phytochemical contents, antioxidant and antibacterial activities of aqueous extract, methanol extract and its fractions obtained from the leaves of D. suffruticosa. The dried leaves were extracted using methanol before successive solvent partitioning was carried out on the extract using hexane, chloroform, ethyl acetate and diethyl ether. In addition to this, aqueous decoction was conducted. The antioxidant activities were determined using Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging methods. Phytochemical screening had shown that most of the extracts and fractions contained alkaloids, steroids, phenolics, flavonoids and saponins. The diethyl ether and ethyl acetate fractions showed higher TPC and TFC values. The diethyl ether and ethyl acetate fractions also showed higher antioxidant activities determined via DPPH assay. Antibacterial activities determined using disc diffusion assay showed the methanol extract and its fractions had antibacterial activity against Staphylococcus aureus, with the diethyl ether fraction having comparable activity with the standard antibiotic streptomycin. However, inhibition against Bacillus subtilis was only observed in hexane, chloroform and diethyl ether fractions. No inhibition was observed against Escherichia coli and Pseudomonas aeruginosa. This study identified the diethyl ethyl and ethyl acetate fractions of D. suffruticosa leaves as potential sources of bioactive compounds. Further investigations on the bioassay-guided isolation of bioactive compounds of this species may lead to the discovery of new pharmaceutical or effective antimicrobial agents.
format Article
author Farazimah Yakop,
Malai Haniti Sheikh Abdul Hamid,
Norhayati Ahmad,
Mohamed Abdul Majid,
Manoharan Karuppiah Pillai,
Hussein Taha,
spellingShingle Farazimah Yakop,
Malai Haniti Sheikh Abdul Hamid,
Norhayati Ahmad,
Mohamed Abdul Majid,
Manoharan Karuppiah Pillai,
Hussein Taha,
Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
author_facet Farazimah Yakop,
Malai Haniti Sheikh Abdul Hamid,
Norhayati Ahmad,
Mohamed Abdul Majid,
Manoharan Karuppiah Pillai,
Hussein Taha,
author_sort Farazimah Yakop,
title Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
title_short Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
title_full Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
title_fullStr Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
title_full_unstemmed Phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of Dillenia suffruticosa leaves
title_sort phytochemical screening, antioxidant and antibacterial activities of extracts and fractions of dillenia suffruticosa leaves
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/15707/1/49_01_14.pdf
http://journalarticle.ukm.my/15707/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=981&catid=59:current-view&Itemid=56
_version_ 1684654291081494528
score 13.211869

Similar Items