High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves
Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1 H-N...
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2020
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my-ukm.journal.156232020-11-11T16:32:49Z http://journalarticle.ukm.my/15623/ High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang, Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20) extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1 H-NMR results were used to determine the peak that exists for the group compounds. Chromatographic peaks were detected and integrated by the MassHunter Acquisition B.07.00 for the Agilent TOF and QTOF and MassHunter Qualitative Analysis B.07.00. The ethanol:water (80:20) extract of L. inermis have shown, predominantly, the presence of phenolic compounds (coumarins, flavonoids, naphthalene, and gallic acid) which are highly glycosylated. The presence of compounds such as apiin, lawsone, apigenin, luteolin, cosmosiin, and p-coumaric acid were also found. Penerbit Universiti Kebangsaan Malaysia 2020-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15623/1/12.pdf Muhammad Remy Othman, and Rozana Othman, and Abdul Aziz Ismail, and Hazrina Hazni, and Khadher Ahmad, and Munirah Abd Razzak, and Zulkifli Mohd Yusoff, and Khalijah Awang, (2020) High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves. Sains Malaysiana, 49 (7). pp. 1597-1613. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html |
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Lawsonia inermis (Henna), (Lythraceae), contains a high amount of phenolic compounds which could activate
antioxidants to help reduce free radicals. In this study, the compound content found in the ethanol:water (80:20)
extract of local Lawsonia inermis was determined using HPLC-QTOFMS. The 1
H-NMR results were used to determine the
peak that exists for the group compounds. Chromatographic peaks were detected and integrated by the MassHunter
Acquisition B.07.00 for the Agilent TOF and QTOF and MassHunter Qualitative Analysis B.07.00. The ethanol:water
(80:20) extract of L. inermis have shown, predominantly, the presence of phenolic compounds (coumarins, flavonoids,
naphthalene, and gallic acid) which are highly glycosylated. The presence of compounds such as apiin, lawsone,
apigenin, luteolin, cosmosiin, and p-coumaric acid were also found. |
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Article |
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Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang, |
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Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang, High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
author_facet |
Muhammad Remy Othman, Rozana Othman, Abdul Aziz Ismail, Hazrina Hazni, Khadher Ahmad, Munirah Abd Razzak, Zulkifli Mohd Yusoff, Khalijah Awang, |
author_sort |
Muhammad Remy Othman, |
title |
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
title_short |
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
title_full |
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
title_fullStr |
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
title_full_unstemmed |
High-performance liquid chromatography quadrupole time-of-flight mass spectrometry (HPLC-QTOFMS) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
title_sort |
high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (hplc-qtofms) analysis on the ethanol:water (80:20) extract of lawsonia inermis leaves |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2020 |
url |
http://journalarticle.ukm.my/15623/1/12.pdf http://journalarticle.ukm.my/15623/ http://www.ukm.my/jsm/malay_journals/jilid49bil7_2020/KandunganJilid49Bil7_2020.html |
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1683231769595215872 |
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13.211869 |