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Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase

Palm oil tocotrienol-rich fraction (TRF) containing majorly of αα, β, γ andδδ-tocotrienols and some αα-tocopherols, was reported to have anti-ageing effects in both human and non-human model organisms, but still remains unexplored in the yeast model. It was reported to have the ability to extend the...

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Main Authors: Khaizurin Tajul Arifin,, Tuan, Sheng G, Muhammed Whisz QR,, Blitzer BM,, Saidatul Akmaliah A,, Nurliza AJ,, Pei, Jia L, Rachael Shristi W,
Format: Article
Language:English
Published: Pusat Perubatan Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/15217/1/9_ms449_pdf_21205.pdf
http://journalarticle.ukm.my/15217/
http://www.medicineandhealthukm.com/toc/14/1
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spelling my-ukm.journal.152172020-09-21T04:56:58Z http://journalarticle.ukm.my/15217/ Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase Khaizurin Tajul Arifin, Tuan, Sheng G Muhammed Whisz QR, Blitzer BM, Saidatul Akmaliah A, Nurliza AJ, Pei, Jia L Rachael Shristi W, Palm oil tocotrienol-rich fraction (TRF) containing majorly of αα, β, γ andδδ-tocotrienols and some αα-tocopherols, was reported to have anti-ageing effects in both human and non-human model organisms, but still remains unexplored in the yeast model. It was reported to have the ability to extend the lifespan of several organisms. Chronological lifespan is one of the means to measure ageing in yeasts. The effect of TRF on the viability of three strains of Saccharomyces cerevisiae (wild-type, CTT1ΔΔand GPx2Δ) was studied. Phenotypic growth analysis of all strains was carried out for 15 hours by measuring the absorbance at OD600nm and cell counting. The optimum dose of TRF was optimised by determining the number of colony-forming unit by the wild-type strain at the end of a 24-hour treatment with TRF (ranging from 0μg/ml to 300μg/ml). TRF at 300 μg/mL showed the best result, and selected as a working dose. Treatment of cells with 300 μg/mL of TRF improved the viability of the wild-type strain in the initial stationary phase, but not on the knockout strains. These finding suggests that TRF has a potential in prolonging the chronological lifespan of S. cerevisiae, and perhaps other organisms as well. Pusat Perubatan Universiti Kebangsaan Malaysia 2019 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/15217/1/9_ms449_pdf_21205.pdf Khaizurin Tajul Arifin, and Tuan, Sheng G and Muhammed Whisz QR, and Blitzer BM, and Saidatul Akmaliah A, and Nurliza AJ, and Pei, Jia L and Rachael Shristi W, (2019) Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase. Medicine & Health, 14 (1). pp. 106-117. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/14/1
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Palm oil tocotrienol-rich fraction (TRF) containing majorly of αα, β, γ andδδ-tocotrienols and some αα-tocopherols, was reported to have anti-ageing effects in both human and non-human model organisms, but still remains unexplored in the yeast model. It was reported to have the ability to extend the lifespan of several organisms. Chronological lifespan is one of the means to measure ageing in yeasts. The effect of TRF on the viability of three strains of Saccharomyces cerevisiae (wild-type, CTT1ΔΔand GPx2Δ) was studied. Phenotypic growth analysis of all strains was carried out for 15 hours by measuring the absorbance at OD600nm and cell counting. The optimum dose of TRF was optimised by determining the number of colony-forming unit by the wild-type strain at the end of a 24-hour treatment with TRF (ranging from 0μg/ml to 300μg/ml). TRF at 300 μg/mL showed the best result, and selected as a working dose. Treatment of cells with 300 μg/mL of TRF improved the viability of the wild-type strain in the initial stationary phase, but not on the knockout strains. These finding suggests that TRF has a potential in prolonging the chronological lifespan of S. cerevisiae, and perhaps other organisms as well.
format Article
author Khaizurin Tajul Arifin,
Tuan, Sheng G
Muhammed Whisz QR,
Blitzer BM,
Saidatul Akmaliah A,
Nurliza AJ,
Pei, Jia L
Rachael Shristi W,
spellingShingle Khaizurin Tajul Arifin,
Tuan, Sheng G
Muhammed Whisz QR,
Blitzer BM,
Saidatul Akmaliah A,
Nurliza AJ,
Pei, Jia L
Rachael Shristi W,
Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
author_facet Khaizurin Tajul Arifin,
Tuan, Sheng G
Muhammed Whisz QR,
Blitzer BM,
Saidatul Akmaliah A,
Nurliza AJ,
Pei, Jia L
Rachael Shristi W,
author_sort Khaizurin Tajul Arifin,
title Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
title_short Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
title_full Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
title_fullStr Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
title_full_unstemmed Tocotrienol-rich fraction (TRF) improves the viability of wild-type Saccharomyces cerevisiae in the initial stationary phase
title_sort tocotrienol-rich fraction (trf) improves the viability of wild-type saccharomyces cerevisiae in the initial stationary phase
publisher Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/15217/1/9_ms449_pdf_21205.pdf
http://journalarticle.ukm.my/15217/
http://www.medicineandhealthukm.com/toc/14/1
_version_ 1678593697459994624
score 13.211869

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