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Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish

Obesity is a major factor associated with inflammatory and various metabolic syndromes. Etlingera caloprhys, an Indonesia plant native from Southeast Sulawesi, is traditionally used years as daily food ingredient by local communities. The present paper presents an in vitro and in vivo study related...

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Main Authors: Idrus, Loly Subhiaty, Fakri, Fajar, Rika Hartati,, Vienna Saraswaty,, I Ketut Adnyana,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2020
Online Access:http://journalarticle.ukm.my/14771/1/ARTIKEL%2017.pdf
http://journalarticle.ukm.my/14771/
http://www.ukm.my/jsm/malay_journals/jilid49bil2_2020/KandunganJilid49Bil2_2020.html
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spelling my-ukm.journal.147712020-06-22T08:42:14Z http://journalarticle.ukm.my/14771/ Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish Idrus, Loly Subhiaty Fakri, Fajar Rika Hartati, Vienna Saraswaty, I Ketut Adnyana, Obesity is a major factor associated with inflammatory and various metabolic syndromes. Etlingera caloprhys, an Indonesia plant native from Southeast Sulawesi, is traditionally used years as daily food ingredient by local communities. The present paper presents an in vitro and in vivo study related to the anti-obesity effects of Etlingera caloprhys ethanol extract (ECEE). An experiment was conducted to evaluate in vitro pancreatic lipase enzyme (PLE) inhibition and in vivo anti-obesity activity in high fat diet (HFD) induced obese zebrafish fed with 10% w/w and 20% w/w ECEE-HFD were evaluated. To confirm anti-obesity activity, a set of obesity and biochemistry parameters i.e. body weight, body mass index (BMI), triglyceride and blood glucose levels, were monitored. Additionally, gene expression levels related to obesity i.e. adiponectin and PPARγ in visceral adipose tissue (VAT) were also evaluated. The results showed that ECEE significantly (p <0.05) inhibited PLE activity with an inhibition concentration (IC)50 of below 100 μg/mL. The HFD induced obese zebrafish fed with 10% w/w and 20% w/w ECEE-HFD showed decreasing body weight and BMI. The biochemistry parameters of the HFD-induced obese zebrafish fed with 10% w/w ECEE-HFD obviously displayed optimum reduction of triglycerides and blood glucose levels. More importantly, the gene expression confirmed that ECEE suppressed adipogenesis in VAT. Hence, ECEE is suggested as a potent alternative medicine for prevention of metabolic syndromes related to obesity. Penerbit Universiti Kebangsaan Malaysia 2020-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14771/1/ARTIKEL%2017.pdf Idrus, Loly Subhiaty and Fakri, Fajar and Rika Hartati, and Vienna Saraswaty, and I Ketut Adnyana, (2020) Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish. Sains Malaysiana, 49 (2). pp. 389-397. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid49bil2_2020/KandunganJilid49Bil2_2020.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Obesity is a major factor associated with inflammatory and various metabolic syndromes. Etlingera caloprhys, an Indonesia plant native from Southeast Sulawesi, is traditionally used years as daily food ingredient by local communities. The present paper presents an in vitro and in vivo study related to the anti-obesity effects of Etlingera caloprhys ethanol extract (ECEE). An experiment was conducted to evaluate in vitro pancreatic lipase enzyme (PLE) inhibition and in vivo anti-obesity activity in high fat diet (HFD) induced obese zebrafish fed with 10% w/w and 20% w/w ECEE-HFD were evaluated. To confirm anti-obesity activity, a set of obesity and biochemistry parameters i.e. body weight, body mass index (BMI), triglyceride and blood glucose levels, were monitored. Additionally, gene expression levels related to obesity i.e. adiponectin and PPARγ in visceral adipose tissue (VAT) were also evaluated. The results showed that ECEE significantly (p <0.05) inhibited PLE activity with an inhibition concentration (IC)50 of below 100 μg/mL. The HFD induced obese zebrafish fed with 10% w/w and 20% w/w ECEE-HFD showed decreasing body weight and BMI. The biochemistry parameters of the HFD-induced obese zebrafish fed with 10% w/w ECEE-HFD obviously displayed optimum reduction of triglycerides and blood glucose levels. More importantly, the gene expression confirmed that ECEE suppressed adipogenesis in VAT. Hence, ECEE is suggested as a potent alternative medicine for prevention of metabolic syndromes related to obesity.
format Article
author Idrus, Loly Subhiaty
Fakri, Fajar
Rika Hartati,
Vienna Saraswaty,
I Ketut Adnyana,
spellingShingle Idrus, Loly Subhiaty
Fakri, Fajar
Rika Hartati,
Vienna Saraswaty,
I Ketut Adnyana,
Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
author_facet Idrus, Loly Subhiaty
Fakri, Fajar
Rika Hartati,
Vienna Saraswaty,
I Ketut Adnyana,
author_sort Idrus, Loly Subhiaty
title Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
title_short Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
title_full Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
title_fullStr Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
title_full_unstemmed Ameliorative effect of Etlingera calophrys (K.Schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
title_sort ameliorative effect of etlingera calophrys (k.schum.) rhizome ethanolic extract on high fat diet-induced obese zebrafish
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2020
url http://journalarticle.ukm.my/14771/1/ARTIKEL%2017.pdf
http://journalarticle.ukm.my/14771/
http://www.ukm.my/jsm/malay_journals/jilid49bil2_2020/KandunganJilid49Bil2_2020.html
_version_ 1671340602395983872
score 13.211869

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