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Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells

Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosi...

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Main Authors: Shawal Maradona Abdul Wahab,, Zariyantey Abd Hamid,, Ramya Dewi Mathialagan,, Izatus Shima Taib,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf
http://journalarticle.ukm.my/14348/
http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
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spelling my-ukm.journal.143482020-03-03T08:02:26Z http://journalarticle.ukm.my/14348/ Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells Shawal Maradona Abdul Wahab, Zariyantey Abd Hamid, Ramya Dewi Mathialagan, Izatus Shima Taib, Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosis and cell loss. This study investigated impact of supplementing N-acetylcysteine (NAC) as antioxidant during cryopreservation on viability and oxidative stress in HSCs. HSCs were isolated from murine bone marrow, cultured in HSCs growth media and cryopreserved (1×106 cells per vial) together with 10% DMSO and NAC (0, 0.25, 0.5 or 2.0 μM) for 48 h, 2 weeks or 8 weeks at -196°C using controlled-rate-freezing technique. Cell viability and oxidative stress in cryopreserved cells were analysed at each time-point. Cell viability was significantly reduced (p<0.05) following cryopreservation as compared to pre-cryopreservation. NAC supplementation significantly increased cell viability (p<0.05) after 48 h cryopreservation at 0.5 μM and 2.0 μM and after 2 weeks cryopreservation at 0.25 μM compared to the controls. Cryopreservation significantly enhanced GSH level (p<0.05) and reduced MDA level (p<0.05) without affecting SOD activity and PC level in HSCs compared to pre-cryopreservation. NAC supplementation significantly increased GSH level at 0.25 μM in cryopreserved HSCs compared to control. In conclusion, NAC supplementation during cryopreservation showed potential in minimizing cryoinjury by promoting cell viability, increasing antioxidant capacity and reducing oxidative damage in HSCs, however these effects are influenced by both durations of cryopreservation and NAC concentration. Penerbit Universiti Kebangsaan Malaysia 2019-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf Shawal Maradona Abdul Wahab, and Zariyantey Abd Hamid, and Ramya Dewi Mathialagan, and Izatus Shima Taib, (2019) Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells. Sains Malaysiana, 48 (9). pp. 1937-1946. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Hematopoietic stem cells (HSCs) transplantation was introduced as curative treatment for various diseases. Cryopreservation of HSCs is crucial for long term storage and maintenance of cellular quality; however, it has been reported that cryopreservation itself causes oxidative stress-driven apoptosis and cell loss. This study investigated impact of supplementing N-acetylcysteine (NAC) as antioxidant during cryopreservation on viability and oxidative stress in HSCs. HSCs were isolated from murine bone marrow, cultured in HSCs growth media and cryopreserved (1×106 cells per vial) together with 10% DMSO and NAC (0, 0.25, 0.5 or 2.0 μM) for 48 h, 2 weeks or 8 weeks at -196°C using controlled-rate-freezing technique. Cell viability and oxidative stress in cryopreserved cells were analysed at each time-point. Cell viability was significantly reduced (p<0.05) following cryopreservation as compared to pre-cryopreservation. NAC supplementation significantly increased cell viability (p<0.05) after 48 h cryopreservation at 0.5 μM and 2.0 μM and after 2 weeks cryopreservation at 0.25 μM compared to the controls. Cryopreservation significantly enhanced GSH level (p<0.05) and reduced MDA level (p<0.05) without affecting SOD activity and PC level in HSCs compared to pre-cryopreservation. NAC supplementation significantly increased GSH level at 0.25 μM in cryopreserved HSCs compared to control. In conclusion, NAC supplementation during cryopreservation showed potential in minimizing cryoinjury by promoting cell viability, increasing antioxidant capacity and reducing oxidative damage in HSCs, however these effects are influenced by both durations of cryopreservation and NAC concentration.
format Article
author Shawal Maradona Abdul Wahab,
Zariyantey Abd Hamid,
Ramya Dewi Mathialagan,
Izatus Shima Taib,
spellingShingle Shawal Maradona Abdul Wahab,
Zariyantey Abd Hamid,
Ramya Dewi Mathialagan,
Izatus Shima Taib,
Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
author_facet Shawal Maradona Abdul Wahab,
Zariyantey Abd Hamid,
Ramya Dewi Mathialagan,
Izatus Shima Taib,
author_sort Shawal Maradona Abdul Wahab,
title Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_short Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_full Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_fullStr Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_full_unstemmed Effect of N-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
title_sort effect of n-acetylcysteine supplementation on oxidative stress-mediated cryoinjury of bone marrow derived-hematopoietic stem cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/14348/1/15%20Shawal%20Maradona%20Abdul%20Wahab.pdf
http://journalarticle.ukm.my/14348/
http://www.ukm.my/jsm/malay_journals/jilid48bil9_2019/KandunganJilid48Bil9_2019.html
_version_ 1662755956805599232
score 13.211869

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