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Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences

In Malaysia, stingless bee Heterotrigona itama is one of the bee species that is actively reared and mostly utilized in meliponiculture practice. In this preliminary study, we described the phylogenetic relationship scenario among selected localities of H. itama in Peninsular Malaysia and Borneo. Ge...

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Main Authors: Nur Syafika Mohd-Yusof,, Nik Abdul Rahman Nik-Rashidi,, Nadiatur Akmar Zulkifli,, Salmah Yaakop,, Izfa Riza Hazmi,, Badrul Munir Md-Zain,
Format: Article
Language:English
Published: Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/13948/1/25935-87007-1-PB.pdf
http://journalarticle.ukm.my/13948/
http://ejournals.ukm.my/serangga/issue/view/1133/showToc
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spelling my-ukm.journal.139482020-01-17T08:16:04Z http://journalarticle.ukm.my/13948/ Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences Nur Syafika Mohd-Yusof, Nik Abdul Rahman Nik-Rashidi, Nadiatur Akmar Zulkifli, Salmah Yaakop, Izfa Riza Hazmi, Badrul Munir Md-Zain, In Malaysia, stingless bee Heterotrigona itama is one of the bee species that is actively reared and mostly utilized in meliponiculture practice. In this preliminary study, we described the phylogenetic relationship scenario among selected localities of H. itama in Peninsular Malaysia and Borneo. Genomic DNA from 16 samples of five localities in Peninsular Malaysia (Kedah, Kelantan, Melaka and Johor) and Borneo (Sarawak) was successfully extracted and sequenced for phylogenetic analysis. The 489 bp mitochondrial DNA of COI sequences was generated for phylogenetic tree reconstruction and population genetic analysis. Tree topology indicated two monophyletic clades which belong to Peninsular Malaysia and Sarawak specimens with high bootstrap support. Five population genetic parameters were studied including nucleotide diversity (π), net nucleotide divergence (Da), nucleotide subdivision (Nst), population subdivision (FST) and gene flow (Nm). The Sarawak samples had the highest scores for all five parameters indicating its genetic affinity to the population in Peninsular Malaysia. Haplotype analysis showed that Kedah-Kelantan samples shared the same haplotype while Sarawak samples had its own unique haplotype with no sharing of haplotypes between other localities. Minimum-Spanning Network generated a visual representation of the relationship reflect a clear separation between Sarawak and Peninsular Malaysia stingless bees samples. This preliminary study shows the importance of understanding the systematic data towards conservation efforts of Malaysian stingless bee’s diversity. Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13948/1/25935-87007-1-PB.pdf Nur Syafika Mohd-Yusof, and Nik Abdul Rahman Nik-Rashidi, and Nadiatur Akmar Zulkifli, and Salmah Yaakop, and Izfa Riza Hazmi, and Badrul Munir Md-Zain, (2018) Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences. Serangga, 23 (2(SI)). pp. 36-48. ISSN 1394-5130 http://ejournals.ukm.my/serangga/issue/view/1133/showToc
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description In Malaysia, stingless bee Heterotrigona itama is one of the bee species that is actively reared and mostly utilized in meliponiculture practice. In this preliminary study, we described the phylogenetic relationship scenario among selected localities of H. itama in Peninsular Malaysia and Borneo. Genomic DNA from 16 samples of five localities in Peninsular Malaysia (Kedah, Kelantan, Melaka and Johor) and Borneo (Sarawak) was successfully extracted and sequenced for phylogenetic analysis. The 489 bp mitochondrial DNA of COI sequences was generated for phylogenetic tree reconstruction and population genetic analysis. Tree topology indicated two monophyletic clades which belong to Peninsular Malaysia and Sarawak specimens with high bootstrap support. Five population genetic parameters were studied including nucleotide diversity (π), net nucleotide divergence (Da), nucleotide subdivision (Nst), population subdivision (FST) and gene flow (Nm). The Sarawak samples had the highest scores for all five parameters indicating its genetic affinity to the population in Peninsular Malaysia. Haplotype analysis showed that Kedah-Kelantan samples shared the same haplotype while Sarawak samples had its own unique haplotype with no sharing of haplotypes between other localities. Minimum-Spanning Network generated a visual representation of the relationship reflect a clear separation between Sarawak and Peninsular Malaysia stingless bees samples. This preliminary study shows the importance of understanding the systematic data towards conservation efforts of Malaysian stingless bee’s diversity.
format Article
author Nur Syafika Mohd-Yusof,
Nik Abdul Rahman Nik-Rashidi,
Nadiatur Akmar Zulkifli,
Salmah Yaakop,
Izfa Riza Hazmi,
Badrul Munir Md-Zain,
spellingShingle Nur Syafika Mohd-Yusof,
Nik Abdul Rahman Nik-Rashidi,
Nadiatur Akmar Zulkifli,
Salmah Yaakop,
Izfa Riza Hazmi,
Badrul Munir Md-Zain,
Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
author_facet Nur Syafika Mohd-Yusof,
Nik Abdul Rahman Nik-Rashidi,
Nadiatur Akmar Zulkifli,
Salmah Yaakop,
Izfa Riza Hazmi,
Badrul Munir Md-Zain,
author_sort Nur Syafika Mohd-Yusof,
title Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
title_short Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
title_full Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
title_fullStr Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
title_full_unstemmed Phylogenetic relationships of Heterotrigona itama in Malaysia based on COI DNA sequences
title_sort phylogenetic relationships of heterotrigona itama in malaysia based on coi dna sequences
publisher Pusat Sistematik Serangga, Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/13948/1/25935-87007-1-PB.pdf
http://journalarticle.ukm.my/13948/
http://ejournals.ukm.my/serangga/issue/view/1133/showToc
_version_ 1657565444120248320
score 13.211869

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