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Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens

The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluat...

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Main Authors: Rashidah Abdul Razak,, Mohamed Shariff,, Fatimah Md. Yusoff,, Intan Safinar Ismail,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13882/1/05%20Rashidah%20Abdul%20Razak.pdf
http://journalarticle.ukm.my/13882/
http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
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spelling my-ukm.journal.138822020-01-10T08:37:31Z http://journalarticle.ukm.my/13882/ Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens Rashidah Abdul Razak, Mohamed Shariff, Fatimah Md. Yusoff, Intan Safinar Ismail, The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluated the bactericidal efficacy of crude methanolic and aqueous extracts from Polygonum chinense, Syzygium polyanthum, Premna foetida, Pimenta dioica, Brucea javanica, Vitex negundo, Alpinia conchigera and Clinacanthus nutans against Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus and Aeromonas hydrophila using disc diffusion method. The results showed that methanolic extracts of P. dioica, P. foetida and P. chinense, and aqueous extracts of P. dioica and S. polyanthum showed moderate to strong activity (10.8 to 17.2 mm) against all the tested bacteria. These five potential crude extracts were fractionated using liquid-liquid extraction method to obtain the methanol, dichloromethane and ethyl acetate fractions. Among the fractions, ethyl acetate fraction showed the highest activity against all tested bacteria, with minimum inhibition concentration (MIC) values between 0.625 and 10.000 mg/mL. In addition, the five potential crude extracts had low to moderate toxicity with LC50 >100 μg/mL using brine shrimp cytotoxicity assays. The results of this study indicated that methanolic extracts of P. chinense and P. foetida that showed high bactericidal activity and low toxicity could be good potentials for use in fish culture. Penerbit Universiti Kebangsaan Malaysia 2019-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13882/1/05%20Rashidah%20Abdul%20Razak.pdf Rashidah Abdul Razak, and Mohamed Shariff, and Fatimah Md. Yusoff, and Intan Safinar Ismail, (2019) Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens. Sains Malaysiana, 48 (8). pp. 1601-1608. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The emergence of new diseases and the increased use of antibiotics have led to the development of resistant bacterial strains. Thus, there is greater attention to seek new antibacterial agents from the natural sources for combating fish diseases in the aquaculture industry. The present study evaluated the bactericidal efficacy of crude methanolic and aqueous extracts from Polygonum chinense, Syzygium polyanthum, Premna foetida, Pimenta dioica, Brucea javanica, Vitex negundo, Alpinia conchigera and Clinacanthus nutans against Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus and Aeromonas hydrophila using disc diffusion method. The results showed that methanolic extracts of P. dioica, P. foetida and P. chinense, and aqueous extracts of P. dioica and S. polyanthum showed moderate to strong activity (10.8 to 17.2 mm) against all the tested bacteria. These five potential crude extracts were fractionated using liquid-liquid extraction method to obtain the methanol, dichloromethane and ethyl acetate fractions. Among the fractions, ethyl acetate fraction showed the highest activity against all tested bacteria, with minimum inhibition concentration (MIC) values between 0.625 and 10.000 mg/mL. In addition, the five potential crude extracts had low to moderate toxicity with LC50 >100 μg/mL using brine shrimp cytotoxicity assays. The results of this study indicated that methanolic extracts of P. chinense and P. foetida that showed high bactericidal activity and low toxicity could be good potentials for use in fish culture.
format Article
author Rashidah Abdul Razak,
Mohamed Shariff,
Fatimah Md. Yusoff,
Intan Safinar Ismail,
spellingShingle Rashidah Abdul Razak,
Mohamed Shariff,
Fatimah Md. Yusoff,
Intan Safinar Ismail,
Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
author_facet Rashidah Abdul Razak,
Mohamed Shariff,
Fatimah Md. Yusoff,
Intan Safinar Ismail,
author_sort Rashidah Abdul Razak,
title Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_short Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_full Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_fullStr Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_full_unstemmed Bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
title_sort bactericidal efficacy of selected medicinal plant crude extracts and their fractions against common fish pathogens
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13882/1/05%20Rashidah%20Abdul%20Razak.pdf
http://journalarticle.ukm.my/13882/
http://www.ukm.my/jsm/malay_journals/jilid48bil8_2019/KandunganJilid48Bil8_2019.html
_version_ 1657565433466716160
score 13.211869

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