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Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect

Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. A...

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Main Authors: Fariza Akmal Abdul Mutalib,, Jamaliah Md Jahim,, Abdul Wahab MohammadDepartment,, Farah Diba Abu Bakar,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/13794/1/8.pdf
http://journalarticle.ukm.my/13794/
http://www.ukm.my/jkukm/volume-302-2018/
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spelling my-ukm.journal.137942019-12-19T23:48:04Z http://journalarticle.ukm.my/13794/ Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect Fariza Akmal Abdul Mutalib, Jamaliah Md Jahim, Abdul Wahab MohammadDepartment, Farah Diba Abu Bakar, Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. After primary phase separation, more LS54 (polymer) which is the system’s component itself were added into polymer-enriched phase and mixed thoroughly before thermo-induced separation step proceeded. The compositions of LS54 added into the polymer-enriched phase were 0.25, 0.5 and 0.75 g LS54/g top phase. The thermo-induced phase separation was carried out at 37°C. It was found that cutinase recovery in water-enriched phase was increased up to 5-13% with the increment of polymer concentration in the system as compared to a system without polymer addition. The optimum concentration obtained for the polymer added was 0.5 g LS54/g top phase whereby it attained 82% recovery of cutinase enzyme in water-enriched phase after thermoseparation step. Although the increment of enzyme recovery was not exceptionally high as compared to another method such as adding ligand, an affinity tag or neutral salt, still this method is applicable because of its more straightforward work, polymer recycle capability, and enzyme recovery in water phase would definitely give benefit to further downstream processing. Penerbit Universiti Kebangsaan Malaysia 2018-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13794/1/8.pdf Fariza Akmal Abdul Mutalib, and Jamaliah Md Jahim, and Abdul Wahab MohammadDepartment, and Farah Diba Abu Bakar, (2018) Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect. Jurnal Kejuruteraan, 30 (2). pp. 187-192. ISSN 0128-0198 http://www.ukm.my/jkukm/volume-302-2018/
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Low recovery of cutinase enzyme in water-enriched phase after thermo-induced separation stage of LS54/Dx aqueous two-phase system was improved by enhanced volume exclusion effect in the polymer-water extraction system. It was done by increased the polymer concentration in the polymer-water system. After primary phase separation, more LS54 (polymer) which is the system’s component itself were added into polymer-enriched phase and mixed thoroughly before thermo-induced separation step proceeded. The compositions of LS54 added into the polymer-enriched phase were 0.25, 0.5 and 0.75 g LS54/g top phase. The thermo-induced phase separation was carried out at 37°C. It was found that cutinase recovery in water-enriched phase was increased up to 5-13% with the increment of polymer concentration in the system as compared to a system without polymer addition. The optimum concentration obtained for the polymer added was 0.5 g LS54/g top phase whereby it attained 82% recovery of cutinase enzyme in water-enriched phase after thermoseparation step. Although the increment of enzyme recovery was not exceptionally high as compared to another method such as adding ligand, an affinity tag or neutral salt, still this method is applicable because of its more straightforward work, polymer recycle capability, and enzyme recovery in water phase would definitely give benefit to further downstream processing.
format Article
author Fariza Akmal Abdul Mutalib,
Jamaliah Md Jahim,
Abdul Wahab MohammadDepartment,
Farah Diba Abu Bakar,
spellingShingle Fariza Akmal Abdul Mutalib,
Jamaliah Md Jahim,
Abdul Wahab MohammadDepartment,
Farah Diba Abu Bakar,
Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
author_facet Fariza Akmal Abdul Mutalib,
Jamaliah Md Jahim,
Abdul Wahab MohammadDepartment,
Farah Diba Abu Bakar,
author_sort Fariza Akmal Abdul Mutalib,
title Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_short Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_full Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_fullStr Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_full_unstemmed Optimising cutinase enzyme recovery in thermo-induced phase separation of LS54/DX ATPS by enhanced volume exclusion effect
title_sort optimising cutinase enzyme recovery in thermo-induced phase separation of ls54/dx atps by enhanced volume exclusion effect
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/13794/1/8.pdf
http://journalarticle.ukm.my/13794/
http://www.ukm.my/jkukm/volume-302-2018/
_version_ 1654961131318411264
score 13.211869

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