Cover Image

Development of an HPLC method for formic acid analysis through peak exclusion approach

High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specif...

Full description

Saved in:
Bibliographic Details
Main Authors: Hasan, Daniyal, Mahmood, Iram, Ahmad, Ishfaq, Aziz, Farah, Ahmad, Ishaq
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf
http://journalarticle.ukm.my/13622/
http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.13622
record_format eprints
spelling my-ukm.journal.136222019-11-10T04:07:37Z http://journalarticle.ukm.my/13622/ Development of an HPLC method for formic acid analysis through peak exclusion approach Hasan, Daniyal Mahmood, Iram Ahmad, Ishfaq Aziz, Farah Ahmad, Ishaq High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specifically reported expensive HPLC apparatus is not available for every set of mixtures. Present study concentrated on analysis of formic acid from a mixture of methanol, formaldehyde and water. Formic acid is a basic carboxylic acid that has growing industrial and commercial uses, especially as a refrigerant. A C-18 column was utilized for the analysis by optimizing the parameters like dimensions of the stationary column, nature of mobile phase, flow rate of mobile phase and wavelength of the ultraviolet detector. Comprehendible and reproducible results for the analysis of formic acid were obtained on 25 cm long C-18 stationary column, by tuning the combined effect of water as mobile phase at a flow rate of 1 mL/min and by excluding the unnecessary peaks at 250 nm wavelength of ultraviolet detector, called peak exclusion approach. The calibration curve was found to be linear over the range of 1.25 % v/v formic acid solution in methanol up to 100 % pure formic acid. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 2.25 % and 6.83 %, respectively. Penerbit Universiti Kebangsaan Malaysia 2019-05 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf Hasan, Daniyal and Mahmood, Iram and Ahmad, Ishfaq and Aziz, Farah and Ahmad, Ishaq (2019) Development of an HPLC method for formic acid analysis through peak exclusion approach. Sains Malaysiana, 48 (5). pp. 1011-1018. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description High Performance Liquid Chromatography (HPLC) is a chemical analysis technique that gives both the quantitative and qualitative analysis of liquid samples. A certain set of parameters, called HPLC method, are defined for analysis of every discrete mixture. Often literature is not available or specifically reported expensive HPLC apparatus is not available for every set of mixtures. Present study concentrated on analysis of formic acid from a mixture of methanol, formaldehyde and water. Formic acid is a basic carboxylic acid that has growing industrial and commercial uses, especially as a refrigerant. A C-18 column was utilized for the analysis by optimizing the parameters like dimensions of the stationary column, nature of mobile phase, flow rate of mobile phase and wavelength of the ultraviolet detector. Comprehendible and reproducible results for the analysis of formic acid were obtained on 25 cm long C-18 stationary column, by tuning the combined effect of water as mobile phase at a flow rate of 1 mL/min and by excluding the unnecessary peaks at 250 nm wavelength of ultraviolet detector, called peak exclusion approach. The calibration curve was found to be linear over the range of 1.25 % v/v formic acid solution in methanol up to 100 % pure formic acid. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 2.25 % and 6.83 %, respectively.
format Article
author Hasan, Daniyal
Mahmood, Iram
Ahmad, Ishfaq
Aziz, Farah
Ahmad, Ishaq
spellingShingle Hasan, Daniyal
Mahmood, Iram
Ahmad, Ishfaq
Aziz, Farah
Ahmad, Ishaq
Development of an HPLC method for formic acid analysis through peak exclusion approach
author_facet Hasan, Daniyal
Mahmood, Iram
Ahmad, Ishfaq
Aziz, Farah
Ahmad, Ishaq
author_sort Hasan, Daniyal
title Development of an HPLC method for formic acid analysis through peak exclusion approach
title_short Development of an HPLC method for formic acid analysis through peak exclusion approach
title_full Development of an HPLC method for formic acid analysis through peak exclusion approach
title_fullStr Development of an HPLC method for formic acid analysis through peak exclusion approach
title_full_unstemmed Development of an HPLC method for formic acid analysis through peak exclusion approach
title_sort development of an hplc method for formic acid analysis through peak exclusion approach
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13622/1/09%20Daniyal%20Hasan%2C%20Iram%20Mahmood.pdf
http://journalarticle.ukm.my/13622/
http://www.ukm.my/jsm/malay_journals/jilid48bil5_2019/KandunganJilid48Bil5_2019.html
_version_ 1651868040014331904
score 13.211869

Similar Items