Cover Image

Successful and efficient DNA extraction from processed pangolin scales

A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by d...

Full description

Saved in:
Bibliographic Details
Main Authors: Xuan, Cai, Ye, Hao-Ting, Tian, En-Wei, Fang, Li, Chan, Li, Yang, Yao-Jun, Liu, Chun-Sheng, Zhi, Chao
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf
http://journalarticle.ukm.my/13313/
http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.13313
record_format eprints
spelling my-ukm.journal.133132019-08-29T21:28:04Z http://journalarticle.ukm.my/13313/ Successful and efficient DNA extraction from processed pangolin scales Xuan, Cai Ye, Hao-Ting Tian, En-Wei Fang, Li Chan, Li Yang, Yao-Jun Liu, Chun-Sheng Zhi, Chao A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by digestion with proteinase K at 55°C for 120 h. Phenol-chloroform extraction was used to obtain the total DNA. PCR amplification for mitochondrial cytochrome b (cytb) gene was successful using the extracted DNA as the template, and sequencing of the amplified fragments confirmed Manis origin of the scale samples. With an efficiency up to 100%, this method is expected to provide a powerful tool in molecular identification of processed as well as crude pangolin scales. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf Xuan, Cai and Ye, Hao-Ting and Tian, En-Wei and Fang, Li and Chan, Li and Yang, Yao-Jun and Liu, Chun-Sheng and Zhi, Chao (2019) Successful and efficient DNA extraction from processed pangolin scales. Sains Malaysiana, 48 (3). pp. 555-559. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description A successful method for total DNA extraction from crude and processed pangolin scales was established. After pretreatment in the soaking solution for cleansing, the scales were prepared into fine powders and treated with PBS buffer containing 0.1% collagenase and 0.1% trypsin for 24 h, followed by digestion with proteinase K at 55°C for 120 h. Phenol-chloroform extraction was used to obtain the total DNA. PCR amplification for mitochondrial cytochrome b (cytb) gene was successful using the extracted DNA as the template, and sequencing of the amplified fragments confirmed Manis origin of the scale samples. With an efficiency up to 100%, this method is expected to provide a powerful tool in molecular identification of processed as well as crude pangolin scales.
format Article
author Xuan, Cai
Ye, Hao-Ting
Tian, En-Wei
Fang, Li
Chan, Li
Yang, Yao-Jun
Liu, Chun-Sheng
Zhi, Chao
spellingShingle Xuan, Cai
Ye, Hao-Ting
Tian, En-Wei
Fang, Li
Chan, Li
Yang, Yao-Jun
Liu, Chun-Sheng
Zhi, Chao
Successful and efficient DNA extraction from processed pangolin scales
author_facet Xuan, Cai
Ye, Hao-Ting
Tian, En-Wei
Fang, Li
Chan, Li
Yang, Yao-Jun
Liu, Chun-Sheng
Zhi, Chao
author_sort Xuan, Cai
title Successful and efficient DNA extraction from processed pangolin scales
title_short Successful and efficient DNA extraction from processed pangolin scales
title_full Successful and efficient DNA extraction from processed pangolin scales
title_fullStr Successful and efficient DNA extraction from processed pangolin scales
title_full_unstemmed Successful and efficient DNA extraction from processed pangolin scales
title_sort successful and efficient dna extraction from processed pangolin scales
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13313/1/07%20Xuan%20Cai.pdf
http://journalarticle.ukm.my/13313/
http://www.ukm.my/jsm/malay_journals/jilid48bil3_2019/KandunganJilid48Bil3_2019.html
_version_ 1643739039994478592
score 13.211869

Similar Items