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Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea

Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In...

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Main Authors: Jang, Min-Seok, Oh, Myung-Joo, Kim, Seok-Ryel, Kim, Wi-Sik
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf
http://journalarticle.ukm.my/13079/
http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
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spelling my-ukm.journal.130792019-06-20T15:25:28Z http://journalarticle.ukm.my/13079/ Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea Jang, Min-Seok Oh, Myung-Joo Kim, Seok-Ryel Kim, Wi-Sik Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In the present study, a survey was conducted to investigate VHSV infection in 39 olive flounder hatcheries from 2014 to 2017. Fish were tested for the presence of VHSV by inoculating sample to fathead minnow (FHM) and chinook salmon embryo (CHSE-214) cells to observe cytopathic effect, reverse transcription-polymerase chain reaction (RT-PCR), and antibody detection enzyme-linked immunosorbent assay (ELISA). VHSV was not detected in any of the 2,430 fish (from 461 pooled and 156 unpooled samples), although 34 (20.3%) of 167 samples was found to be positive for marine birnavirus (MABV) by cell culture and RT-PCR. Antibody detection ELISA results showed that all 212 fish sera have optical density (OD) values below of 0.1, suggesting that these fish had no VHSV-specific antibodies. Moreover, VHSV was not detected in any of 40 pooled samples (172 fish) collected after shifting rearing water temperature from 17-21°C to 10-15°C. In conclusion, the 39 olive flounder hatcheries surveyed in Korea was not infected by VHSV. Penerbit Universiti Kebangsaan Malaysia 2019-02 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf Jang, Min-Seok and Oh, Myung-Joo and Kim, Seok-Ryel and Kim, Wi-Sik (2019) Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea. Sains Malaysiana, 48 (2). pp. 291-299. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Viral hemorrhagic septicemia (VHS) generally occurs after juvenile olive flounders (Paralichthys olivaceus) are moved from the hatchery to on-growing system in Korea during spring. However, it remains unclear whether fish are infected by VHS virus (VHSV) in the hatchery or the on-growing system. In the present study, a survey was conducted to investigate VHSV infection in 39 olive flounder hatcheries from 2014 to 2017. Fish were tested for the presence of VHSV by inoculating sample to fathead minnow (FHM) and chinook salmon embryo (CHSE-214) cells to observe cytopathic effect, reverse transcription-polymerase chain reaction (RT-PCR), and antibody detection enzyme-linked immunosorbent assay (ELISA). VHSV was not detected in any of the 2,430 fish (from 461 pooled and 156 unpooled samples), although 34 (20.3%) of 167 samples was found to be positive for marine birnavirus (MABV) by cell culture and RT-PCR. Antibody detection ELISA results showed that all 212 fish sera have optical density (OD) values below of 0.1, suggesting that these fish had no VHSV-specific antibodies. Moreover, VHSV was not detected in any of 40 pooled samples (172 fish) collected after shifting rearing water temperature from 17-21°C to 10-15°C. In conclusion, the 39 olive flounder hatcheries surveyed in Korea was not infected by VHSV.
format Article
author Jang, Min-Seok
Oh, Myung-Joo
Kim, Seok-Ryel
Kim, Wi-Sik
spellingShingle Jang, Min-Seok
Oh, Myung-Joo
Kim, Seok-Ryel
Kim, Wi-Sik
Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
author_facet Jang, Min-Seok
Oh, Myung-Joo
Kim, Seok-Ryel
Kim, Wi-Sik
author_sort Jang, Min-Seok
title Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_short Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_full Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_fullStr Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_full_unstemmed Survey of viral hemorrhagic septicemia virus (VHSV) in olive flounder, Paralichthys olivaceus hatchery in Korea
title_sort survey of viral hemorrhagic septicemia virus (vhsv) in olive flounder, paralichthys olivaceus hatchery in korea
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13079/1/04%20Min-Seok%20Jang.pdf
http://journalarticle.ukm.my/13079/
http://www.ukm.my/jsm/english_journals/vol48num2_2019/contentsVol48num2_2019.html
_version_ 1643738973375299584
score 13.211869

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