Cover Image

Long term effect of cryopreservation on primary human skin cells

Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with c...

Full description

Saved in:
Bibliographic Details
Main Authors: Ishak M.F.,, Manira M.,, Ng, Min Hwei, Khairul B.,, Gargy L.,, Aminuddin B.S.,, Ruszymah Haji Idrus,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf
http://journalarticle.ukm.my/13062/
http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.13062
record_format eprints
spelling my-ukm.journal.130622019-06-20T14:39:36Z http://journalarticle.ukm.my/13062/ Long term effect of cryopreservation on primary human skin cells Ishak M.F., Manira M., Ng, Min Hwei Khairul B., Gargy L., Aminuddin B.S., Ruszymah Haji Idrus, Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with consent from patients. The tissue was cleaned, processed and cultured until passage 3. Upon confluency, cells were trypsinised and total cell yield and viability were determined before and after being cryopreserved. Sterility and immunocytochemistry analysis for collagen type I (Col-1) and cytokeratin 14 (CK14) antibodies were also performed on cells cryopreserved for one, three, six and twelve months. There is no significant difference in growth rates for cryopreserved cells for 1 to 12 months, except for fibroblasts at 6 months. Cell viability for both keratinocytes and fibroblasts decreased with time (65%± 3.5% - 89%± 4.5%). Sterility testing showed no contamination after 12 months of cryopreservation. Immunocytochemistry analysis showed positive expression for CK14 (keratinocytes) and Col -1 (fibroblasts) after 12 months of cryopreservation. Morphologically, keratinocytes and fibroblasts were able to retain its phenotype. The loss in viability is consistent in all samples and possibly due to thermal-cycling effect. Immunocytochemistry and consistent cell growth analysis showed that keratinocytes and fibroblasts were able to retain their characteristics in cryopreservation condition. These preliminary findings show that primary skin cells can be stored via cryopreservation and still retain their characteristics. However, further investigations using longer periods of cryopreservation (24 months, 48 months) should be conducted. Penerbit Universiti Kebangsaan Malaysia 2019-01 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf Ishak M.F., and Manira M., and Ng, Min Hwei and Khairul B., and Gargy L., and Aminuddin B.S., and Ruszymah Haji Idrus, (2019) Long term effect of cryopreservation on primary human skin cells. Sains Malaysiana, 48 (1). pp. 137-144. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Cryopreservation is essential for tissue engineering and regenerative medicine. This study was carried out to assess the effect of cryopreservation on skin cells and evaluate the performance of cells after 12 months of cryopreservation. Redundant skin tissue samples were obtained from surgery with consent from patients. The tissue was cleaned, processed and cultured until passage 3. Upon confluency, cells were trypsinised and total cell yield and viability were determined before and after being cryopreserved. Sterility and immunocytochemistry analysis for collagen type I (Col-1) and cytokeratin 14 (CK14) antibodies were also performed on cells cryopreserved for one, three, six and twelve months. There is no significant difference in growth rates for cryopreserved cells for 1 to 12 months, except for fibroblasts at 6 months. Cell viability for both keratinocytes and fibroblasts decreased with time (65%± 3.5% - 89%± 4.5%). Sterility testing showed no contamination after 12 months of cryopreservation. Immunocytochemistry analysis showed positive expression for CK14 (keratinocytes) and Col -1 (fibroblasts) after 12 months of cryopreservation. Morphologically, keratinocytes and fibroblasts were able to retain its phenotype. The loss in viability is consistent in all samples and possibly due to thermal-cycling effect. Immunocytochemistry and consistent cell growth analysis showed that keratinocytes and fibroblasts were able to retain their characteristics in cryopreservation condition. These preliminary findings show that primary skin cells can be stored via cryopreservation and still retain their characteristics. However, further investigations using longer periods of cryopreservation (24 months, 48 months) should be conducted.
format Article
author Ishak M.F.,
Manira M.,
Ng, Min Hwei
Khairul B.,
Gargy L.,
Aminuddin B.S.,
Ruszymah Haji Idrus,
spellingShingle Ishak M.F.,
Manira M.,
Ng, Min Hwei
Khairul B.,
Gargy L.,
Aminuddin B.S.,
Ruszymah Haji Idrus,
Long term effect of cryopreservation on primary human skin cells
author_facet Ishak M.F.,
Manira M.,
Ng, Min Hwei
Khairul B.,
Gargy L.,
Aminuddin B.S.,
Ruszymah Haji Idrus,
author_sort Ishak M.F.,
title Long term effect of cryopreservation on primary human skin cells
title_short Long term effect of cryopreservation on primary human skin cells
title_full Long term effect of cryopreservation on primary human skin cells
title_fullStr Long term effect of cryopreservation on primary human skin cells
title_full_unstemmed Long term effect of cryopreservation on primary human skin cells
title_sort long term effect of cryopreservation on primary human skin cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/13062/1/16%20Ishak%2C%20M.F..pdf
http://journalarticle.ukm.my/13062/
http://www.ukm.my/jsm/malay_journals/jilid48bil1_2019/KandunganJilid48Bil1_2019.html
_version_ 1643738968353669120
score 13.211869

Similar Items