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Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS

Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either ch...

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Main Authors: Lim, Choon Kiat, Rafidah Ahmad,, Nurul Fadhilah Marzuki,, Goh, Yit Kheng, Kamalrul Azlan Azizan,, Goh, You Keng, Goh, Kah Joo
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf
http://journalarticle.ukm.my/12928/
http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
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spelling my-ukm.journal.129282019-05-15T11:07:55Z http://journalarticle.ukm.my/12928/ Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS Lim, Choon Kiat Rafidah Ahmad, Nurul Fadhilah Marzuki, Goh, Yit Kheng Kamalrul Azlan Azizan, Goh, You Keng Goh, Kah Joo Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either chemical or cultural approaches, show varying degrees of effectiveness. The application of biological control agents which is environmental-friendly could be an attractive solution to overcome the problem. Earlier, we had isolated a mycoparasite, Scytalidium parasiticum, from the basidiomata of Ganoderma boninense. In vitro assay and nursery experiment showed that this fungus could suppress Ganoderma infection and reduce disease severity. However, metabolites which might contribute to the antagonistic or mycoparasitic effect remain unknown. In the current study, optimization of fungal sample processing, extraction, and analytical procedures were conducted to obtain metabolites from the maize substrate colonized by mycoparasitic ascomycetous Scytalidium parasiticum. This technique capable of producing sexual spores in sac-like organs. Untargeted metabolomics profiling was carried out by using Liquid Chromatography Time of Flight Mass Spectrometry (LC-ToF-MS). We found that S. parasiticum in both liquid- and solid-state cultivation gave higher metabolite when extracted with 60% methanol with 1% formic acid in combination with homogenisation methods such as ultrasonication and grinding. The findings from this study are useful for optimisation of metabolite extraction from other fungi-Ganoderma-plant interactions. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf Lim, Choon Kiat and Rafidah Ahmad, and Nurul Fadhilah Marzuki, and Goh, Yit Kheng and Kamalrul Azlan Azizan, and Goh, You Keng and Goh, Kah Joo (2018) Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS. Sains Malaysiana, 47 (12). pp. 3061-3068. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Basal stem rot disease of oil palm caused by Ganoderma boninense is one of the most devastating diseases in oil palm plantation resulting in low yield, loss of palm stands and shorter replanting cycle. To-date, there is no effective treatment for Ganoderma infected palms. Control measures, either chemical or cultural approaches, show varying degrees of effectiveness. The application of biological control agents which is environmental-friendly could be an attractive solution to overcome the problem. Earlier, we had isolated a mycoparasite, Scytalidium parasiticum, from the basidiomata of Ganoderma boninense. In vitro assay and nursery experiment showed that this fungus could suppress Ganoderma infection and reduce disease severity. However, metabolites which might contribute to the antagonistic or mycoparasitic effect remain unknown. In the current study, optimization of fungal sample processing, extraction, and analytical procedures were conducted to obtain metabolites from the maize substrate colonized by mycoparasitic ascomycetous Scytalidium parasiticum. This technique capable of producing sexual spores in sac-like organs. Untargeted metabolomics profiling was carried out by using Liquid Chromatography Time of Flight Mass Spectrometry (LC-ToF-MS). We found that S. parasiticum in both liquid- and solid-state cultivation gave higher metabolite when extracted with 60% methanol with 1% formic acid in combination with homogenisation methods such as ultrasonication and grinding. The findings from this study are useful for optimisation of metabolite extraction from other fungi-Ganoderma-plant interactions.
format Article
author Lim, Choon Kiat
Rafidah Ahmad,
Nurul Fadhilah Marzuki,
Goh, Yit Kheng
Kamalrul Azlan Azizan,
Goh, You Keng
Goh, Kah Joo
spellingShingle Lim, Choon Kiat
Rafidah Ahmad,
Nurul Fadhilah Marzuki,
Goh, Yit Kheng
Kamalrul Azlan Azizan,
Goh, You Keng
Goh, Kah Joo
Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
author_facet Lim, Choon Kiat
Rafidah Ahmad,
Nurul Fadhilah Marzuki,
Goh, Yit Kheng
Kamalrul Azlan Azizan,
Goh, You Keng
Goh, Kah Joo
author_sort Lim, Choon Kiat
title Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_short Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_full Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_fullStr Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_full_unstemmed Optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic Scytalidium parasiticum using LC-TOF-MS
title_sort optimization of metabolite extraction protocols for untargeted metabolite profiling of mycoparasitic scytalidium parasiticum using lc-tof-ms
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12928/1/16%20Choon%20Kiat%20Lim.pdf
http://journalarticle.ukm.my/12928/
http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
_version_ 1643738929778655232
score 13.211869

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