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Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells

Neurological diseases are one of the serious health hazards faced by mankind for decades. Neurite outgrowth is a key factor responsible for proper neuronal development. Any misplacement in the process could lead to neurological diseases like Alzheimer’s and Parkinson’s. Treatment with the available...

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Main Authors: Sutin Wansawat,, Mani Iyer Prasanth,, Isidoro, Ciro, Tewin Tencomnao,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12922/1/10%20Sutin%20Wansawat.pdf
http://journalarticle.ukm.my/12922/
http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
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spelling my-ukm.journal.129222019-05-15T11:03:10Z http://journalarticle.ukm.my/12922/ Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells Sutin Wansawat, Mani Iyer Prasanth, Isidoro, Ciro Tewin Tencomnao, Neurological diseases are one of the serious health hazards faced by mankind for decades. Neurite outgrowth is a key factor responsible for proper neuronal development. Any misplacement in the process could lead to neurological diseases like Alzheimer’s and Parkinson’s. Treatment with the available synthetic drugs imparts many difficulties to the patients due to the side effects. Compounds from natural sources can be considered as an effective replacement for this. Mucuna pruriens, used in traditional ayurvedic medicine, contains L-3,4-dihydroxy phenylalanine (L-DOPA) in its seeds, which possesses medicinal effects against neurological diseases. In this regard, seed extracts of M. pruriens originated from Thailand and India, were analyzed for their neuroprotective effects in Neuro2a cells. Hexane, ethyl acetate and ethanol extracts were found to be non-toxic to the viability of the cells. Ethanol extracts of M. pruriens of Thai origin (MTE), hexane extracts of M. pruriens of Indian origin (MIH) and ethyl acetate extracts of M. pruriens of Indian origin (MIEA) were able to induce neurite outgrowth in Neuro2a cells. Interestingly, both MTE and MIH induced neurite outgrowth dependent on Teneurin-4 (Ten-4) transmembrane protein whereas MIEA did the same independent of Ten-4, which was confirmed by real time PCR and gene silencing approach. The present study suggested that M. pruriens can be used as a potential drug in the treatment of neurological diseases as it can induce neurite outgrowth by multiple mechanisms, which will be of great use in the field of medicine. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12922/1/10%20Sutin%20Wansawat.pdf Sutin Wansawat, and Mani Iyer Prasanth, and Isidoro, Ciro and Tewin Tencomnao, (2018) Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells. Sains Malaysiana, 47 (12). pp. 3009-3015. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Neurological diseases are one of the serious health hazards faced by mankind for decades. Neurite outgrowth is a key factor responsible for proper neuronal development. Any misplacement in the process could lead to neurological diseases like Alzheimer’s and Parkinson’s. Treatment with the available synthetic drugs imparts many difficulties to the patients due to the side effects. Compounds from natural sources can be considered as an effective replacement for this. Mucuna pruriens, used in traditional ayurvedic medicine, contains L-3,4-dihydroxy phenylalanine (L-DOPA) in its seeds, which possesses medicinal effects against neurological diseases. In this regard, seed extracts of M. pruriens originated from Thailand and India, were analyzed for their neuroprotective effects in Neuro2a cells. Hexane, ethyl acetate and ethanol extracts were found to be non-toxic to the viability of the cells. Ethanol extracts of M. pruriens of Thai origin (MTE), hexane extracts of M. pruriens of Indian origin (MIH) and ethyl acetate extracts of M. pruriens of Indian origin (MIEA) were able to induce neurite outgrowth in Neuro2a cells. Interestingly, both MTE and MIH induced neurite outgrowth dependent on Teneurin-4 (Ten-4) transmembrane protein whereas MIEA did the same independent of Ten-4, which was confirmed by real time PCR and gene silencing approach. The present study suggested that M. pruriens can be used as a potential drug in the treatment of neurological diseases as it can induce neurite outgrowth by multiple mechanisms, which will be of great use in the field of medicine.
format Article
author Sutin Wansawat,
Mani Iyer Prasanth,
Isidoro, Ciro
Tewin Tencomnao,
spellingShingle Sutin Wansawat,
Mani Iyer Prasanth,
Isidoro, Ciro
Tewin Tencomnao,
Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
author_facet Sutin Wansawat,
Mani Iyer Prasanth,
Isidoro, Ciro
Tewin Tencomnao,
author_sort Sutin Wansawat,
title Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
title_short Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
title_full Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
title_fullStr Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
title_full_unstemmed Mucuna pruriens seed extract promotes neurite outgrowth via Ten-4 dependent and independent mechanisms in neuro2a cells
title_sort mucuna pruriens seed extract promotes neurite outgrowth via ten-4 dependent and independent mechanisms in neuro2a cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12922/1/10%20Sutin%20Wansawat.pdf
http://journalarticle.ukm.my/12922/
http://www.ukm.my/jsm/malay_journals/jilid47bil12_2018/KandunganJilid47Bil12_2018.html
_version_ 1643738928024387584
score 13.211869

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