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Bacterial degradation of palm olein in seawater and identification of some cultivable strains

Palm oil transported in bulk through ocean can increase pollution risks due to accidental spillage or ship collision, especially the refined, bleached and deodorized (RBD) palm olein. Bacterial degradation of RBD palm olein in seawater was investigated as a preliminary finding on palm oil degradat...

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Main Authors: Bhubalan K.,, Hui-Wan, R.A.C., Renganathan P.,, Tamothran A.M.,, Ganesen S.S.K.,, Ghazali R.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/12798/1/48_01_29.pdf
http://journalarticle.ukm.my/12798/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.127982019-04-16T09:11:09Z http://journalarticle.ukm.my/12798/ Bacterial degradation of palm olein in seawater and identification of some cultivable strains Bhubalan K., Hui-Wan, R.A.C. Renganathan P., Tamothran A.M., Ganesen S.S.K., Ghazali R., Palm oil transported in bulk through ocean can increase pollution risks due to accidental spillage or ship collision, especially the refined, bleached and deodorized (RBD) palm olein. Bacterial degradation of RBD palm olein in seawater was investigated as a preliminary finding on palm oil degradation by marine bacteria. The degradation of RBD palm olein was evaluated in seawater in shaken-flask cultures with different oil concentrations. Biochemical oxygen demand (BOD) in the seawater was determined based on changes in dissolved oxygen (DO) values before and at end of 5-day incubation. The concentrations of major fatty acid components in RBD palm olein before and after degradation were determined using gas chromatographyflame ionization detector (GC-FID). Isolated bacteria were screened for lipolytic activity using Spirit Blue Agar before molecular identification. The DO content reduced 20% over a 5-day incubation period and BOD value was determined to be 1.24 mg O2/L based on DO values. The concentrations of fatty acids, namely palmitic acid (C16:0), stearic acid (C18:0) and oleic acid (C18:1) decreased by 53%, 31% and 37%, respectively. The bacterial count increased from 980 CFU/mL during inoculation to 1.8 x 104 CFU/mL on day 5. Five phenotypically different bacterial strains (Pseudoalteromonas gelatinolytica, Staphylococcus haemolyticus, Vibrio harveyi and Vibrio alginolyticus) showed lipolytic activity. This study indicates that marine bacteria utilizes RBD palm olein as substrate, thus degrading it over time. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12798/1/48_01_29.pdf Bhubalan K., and Hui-Wan, R.A.C. and Renganathan P., and Tamothran A.M., and Ganesen S.S.K., and Ghazali R., (2019) Bacterial degradation of palm olein in seawater and identification of some cultivable strains. Malaysian Applied Biology, 48 (1). pp. 207-213. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Palm oil transported in bulk through ocean can increase pollution risks due to accidental spillage or ship collision, especially the refined, bleached and deodorized (RBD) palm olein. Bacterial degradation of RBD palm olein in seawater was investigated as a preliminary finding on palm oil degradation by marine bacteria. The degradation of RBD palm olein was evaluated in seawater in shaken-flask cultures with different oil concentrations. Biochemical oxygen demand (BOD) in the seawater was determined based on changes in dissolved oxygen (DO) values before and at end of 5-day incubation. The concentrations of major fatty acid components in RBD palm olein before and after degradation were determined using gas chromatographyflame ionization detector (GC-FID). Isolated bacteria were screened for lipolytic activity using Spirit Blue Agar before molecular identification. The DO content reduced 20% over a 5-day incubation period and BOD value was determined to be 1.24 mg O2/L based on DO values. The concentrations of fatty acids, namely palmitic acid (C16:0), stearic acid (C18:0) and oleic acid (C18:1) decreased by 53%, 31% and 37%, respectively. The bacterial count increased from 980 CFU/mL during inoculation to 1.8 x 104 CFU/mL on day 5. Five phenotypically different bacterial strains (Pseudoalteromonas gelatinolytica, Staphylococcus haemolyticus, Vibrio harveyi and Vibrio alginolyticus) showed lipolytic activity. This study indicates that marine bacteria utilizes RBD palm olein as substrate, thus degrading it over time.
format Article
author Bhubalan K.,
Hui-Wan, R.A.C.
Renganathan P.,
Tamothran A.M.,
Ganesen S.S.K.,
Ghazali R.,
spellingShingle Bhubalan K.,
Hui-Wan, R.A.C.
Renganathan P.,
Tamothran A.M.,
Ganesen S.S.K.,
Ghazali R.,
Bacterial degradation of palm olein in seawater and identification of some cultivable strains
author_facet Bhubalan K.,
Hui-Wan, R.A.C.
Renganathan P.,
Tamothran A.M.,
Ganesen S.S.K.,
Ghazali R.,
author_sort Bhubalan K.,
title Bacterial degradation of palm olein in seawater and identification of some cultivable strains
title_short Bacterial degradation of palm olein in seawater and identification of some cultivable strains
title_full Bacterial degradation of palm olein in seawater and identification of some cultivable strains
title_fullStr Bacterial degradation of palm olein in seawater and identification of some cultivable strains
title_full_unstemmed Bacterial degradation of palm olein in seawater and identification of some cultivable strains
title_sort bacterial degradation of palm olein in seawater and identification of some cultivable strains
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/12798/1/48_01_29.pdf
http://journalarticle.ukm.my/12798/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
_version_ 1643738890928914432
score 13.211869

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