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Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu

Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic...

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Main Authors: Chai, Jun Meng, Manogiri Sadasivam,, Sevakumaran Vigneswari,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/12797/1/48_01_28.pdf
http://journalarticle.ukm.my/12797/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.127972019-04-16T09:11:55Z http://journalarticle.ukm.my/12797/ Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu Chai, Jun Meng Manogiri Sadasivam, Sevakumaran Vigneswari, Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years. Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp. KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria isolated can be tailored for various biomedical applications. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12797/1/48_01_28.pdf Chai, Jun Meng and Manogiri Sadasivam, and Sevakumaran Vigneswari, (2019) Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu. Malaysian Applied Biology, 48 (1). pp. 199-206. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Polyhydroxyalkanoates (PHA) is a microbial bioplastic accumulated as a storage material under limited growth conditions in the presence of excess carbon sources. The ever increasing concern towards the depletion of petroleum resources and problems with utilization of a growing number of synthetic plastics, PHAs are being considered as a potential substitute for production of conventional non-degradable plastics. PHAs have been developed as biomaterials with unique properties for many years. Among all types of PHA, copolymer poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] is widely sought after for biomedical application due to the biocompatibility, non-cytotoxicity and non-genotoxicity. Therefore, the aim of this study is to isolate and identify P(3HB-co-4HB) producers from water and soil sources in Kuala Terengganu. Samples of lake and soil were collected during November 2017 and then screened for P(3HB-co-4HB) producer. A total of 18 isolates were obtained, however only 5 isolates were identified as potential PHA producer. Interestingly, 3 isolates were confirmed as copolymer P(3HB-co-4HB) producer through gas chromatography analysis. These 3 isolated identified bacterial strain were Cupriavidus sp. TMT 11 with the highest 4HB molar fraction of 14.1%. The other 2 isolates were Acinetobacter sp. KPD 13 and Cupriavidus sp. PD 16. The properties of the copolymer P(3HB-co-4HB) produced by wide variety of bacteria isolated can be tailored for various biomedical applications.
format Article
author Chai, Jun Meng
Manogiri Sadasivam,
Sevakumaran Vigneswari,
spellingShingle Chai, Jun Meng
Manogiri Sadasivam,
Sevakumaran Vigneswari,
Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
author_facet Chai, Jun Meng
Manogiri Sadasivam,
Sevakumaran Vigneswari,
author_sort Chai, Jun Meng
title Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_short Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_full Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_fullStr Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_full_unstemmed Isolation and identification of P(3HB-co-4HB) producing bacteria from various locations in Kuala Terengganu
title_sort isolation and identification of p(3hb-co-4hb) producing bacteria from various locations in kuala terengganu
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/12797/1/48_01_28.pdf
http://journalarticle.ukm.my/12797/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
_version_ 1643738890637410304
score 13.211869

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