Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu

Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are...

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Main Authors: Mohd Ezhar Mohd Noor,, Sharifah Noor Emilia Syed Jamil Fadaak,, Mohd Noor Mat Isa,, Mohd Faizal Abu Bakar,, Muhd Danish-Daniel,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/12793/1/48_01_24.pdf
http://journalarticle.ukm.my/12793/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.127932019-04-16T08:59:18Z http://journalarticle.ukm.my/12793/ Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu Mohd Ezhar Mohd Noor, Sharifah Noor Emilia Syed Jamil Fadaak, Mohd Noor Mat Isa, Mohd Faizal Abu Bakar, Muhd Danish-Daniel, Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are recycled through the “microbial loop”. Thus, understanding the connection between the diversity composition and functional role of aquatic microbial community is crucial for proper lake management. This study aims to determine the diversity and functions of microbial assemblages in Tasik Kenyir by means of shotgun metagenomics analysis. Briefly, water samples were collected from pristine and disturbed areas. Metagenome DNA were then extracted directly and subjected to clone-independent sequencing. Data sequences of all samples were analyzed and functional annotated using bioinformatics software MEGAN6. Analysis showed up to 41 phyla that had been detected from the water samples with the presence of dominant bacterial populations of more than 90% in all samples. Proteobacteria was the most dominant phylum, representing more than 70% of the microbiome in all samples. Other taxa such as Bacteriodetes, Terrabacteria group, Verrucomicrobia, Planctomycetes and Chloroflexi were also found as part of the microbial communities. The first sample from the disturbed area, TKSA1 had 3% of total contigs read assigned to genera Pseudomonas while the other samples appeared to be more homogeneous. The lake also appeared to contain a mixture of autotrophs and heterotrophs capable of performing main biogeochemical cycles. Findings of the present study has provided us valuable information on the microbial diversity structures and their functions in the nutrient processing pathways that occurred in the Tasik Kenyir environment and thus sheds light on the importance of freshwater microbial communities for ecosystem and human health. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12793/1/48_01_24.pdf Mohd Ezhar Mohd Noor, and Sharifah Noor Emilia Syed Jamil Fadaak, and Mohd Noor Mat Isa, and Mohd Faizal Abu Bakar, and Muhd Danish-Daniel, (2019) Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu. Malaysian Applied Biology, 48 (1). pp. 169-175. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Tasik Kenyir located in the state of Terengganu is the largest artificial lake in Southeast Asia with mean-depth of 37 m. This lake plays an important role in maintaining the biodiversity in the surrounding environment. Microbial communities in the lake are important, as most of the nutrients are recycled through the “microbial loop”. Thus, understanding the connection between the diversity composition and functional role of aquatic microbial community is crucial for proper lake management. This study aims to determine the diversity and functions of microbial assemblages in Tasik Kenyir by means of shotgun metagenomics analysis. Briefly, water samples were collected from pristine and disturbed areas. Metagenome DNA were then extracted directly and subjected to clone-independent sequencing. Data sequences of all samples were analyzed and functional annotated using bioinformatics software MEGAN6. Analysis showed up to 41 phyla that had been detected from the water samples with the presence of dominant bacterial populations of more than 90% in all samples. Proteobacteria was the most dominant phylum, representing more than 70% of the microbiome in all samples. Other taxa such as Bacteriodetes, Terrabacteria group, Verrucomicrobia, Planctomycetes and Chloroflexi were also found as part of the microbial communities. The first sample from the disturbed area, TKSA1 had 3% of total contigs read assigned to genera Pseudomonas while the other samples appeared to be more homogeneous. The lake also appeared to contain a mixture of autotrophs and heterotrophs capable of performing main biogeochemical cycles. Findings of the present study has provided us valuable information on the microbial diversity structures and their functions in the nutrient processing pathways that occurred in the Tasik Kenyir environment and thus sheds light on the importance of freshwater microbial communities for ecosystem and human health.
format Article
author Mohd Ezhar Mohd Noor,
Sharifah Noor Emilia Syed Jamil Fadaak,
Mohd Noor Mat Isa,
Mohd Faizal Abu Bakar,
Muhd Danish-Daniel,
spellingShingle Mohd Ezhar Mohd Noor,
Sharifah Noor Emilia Syed Jamil Fadaak,
Mohd Noor Mat Isa,
Mohd Faizal Abu Bakar,
Muhd Danish-Daniel,
Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
author_facet Mohd Ezhar Mohd Noor,
Sharifah Noor Emilia Syed Jamil Fadaak,
Mohd Noor Mat Isa,
Mohd Faizal Abu Bakar,
Muhd Danish-Daniel,
author_sort Mohd Ezhar Mohd Noor,
title Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_short Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_full Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_fullStr Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_full_unstemmed Biodiversity and functional metagenomic profiling of microbial communities in Tasik Kenyir, Terengganu
title_sort biodiversity and functional metagenomic profiling of microbial communities in tasik kenyir, terengganu
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/12793/1/48_01_24.pdf
http://journalarticle.ukm.my/12793/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
_version_ 1643738889450422272
score 13.211869