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Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+

This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 )...

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Main Authors: Hazlina A.Z.,, Devanthiran L.,, Fatimah H.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2019
Online Access:http://journalarticle.ukm.my/12772/1/48_01_05.pdf
http://journalarticle.ukm.my/12772/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.127722019-04-16T08:39:07Z http://journalarticle.ukm.my/12772/ Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+ Hazlina A.Z., Devanthiran L., Fatimah H., This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 ) of Hg2+ on the growth of C. vulgaris obtained from the dose-response curve was 0.72 mg/L. Under SEM, it was observed that Hg2+ treated cells become smaller in size (i.e. 2.1±0.1 μm) compared to normal cells (i.e. 3.2±0.1 μm). The morphology of cells changed from an intact microalgal cells with smooth spherical surface to a slightly roughened surface and shrivelled shape. Apoptotic bodies were also observed under 0.001, 0.01 and 0.1 mg/L of Hg2+ but not in 1.0 mg/L Hg2+. These results indicate that Hg2+ may induce apoptotic cell death at low concentrations but not at the highest concentration of 1.0 mg/L. The highest percentage of cells with comet level of 4 was observed in 1.0 mg/L Hg2+. However, Hg2+ is genotoxic to the microalga even at low concentration. In conclusion, Hg2+ can exert its toxic effects on C. vulgaris by changing the microalgal morphology, damaging the microalgal DNA and inducing cell death in the microalgal cells using different pathways at different concentrations. Penerbit Universiti Kebangsaan Malaysia 2019-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12772/1/48_01_05.pdf Hazlina A.Z., and Devanthiran L., and Fatimah H., (2019) Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+. Malaysian Applied Biology, 48 (1). pp. 27-33. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description This study reported the effects of Hg2+ on the morphology and DNA of a microalga, Chlorella vulgaris (UMT-M1). Morphological changes and DNA damage in cells were analysed using scanning electron microscope (SEM) and Comet assay, respectively. The half maximal inhibitory concentration (i.e. IC50 ) of Hg2+ on the growth of C. vulgaris obtained from the dose-response curve was 0.72 mg/L. Under SEM, it was observed that Hg2+ treated cells become smaller in size (i.e. 2.1±0.1 μm) compared to normal cells (i.e. 3.2±0.1 μm). The morphology of cells changed from an intact microalgal cells with smooth spherical surface to a slightly roughened surface and shrivelled shape. Apoptotic bodies were also observed under 0.001, 0.01 and 0.1 mg/L of Hg2+ but not in 1.0 mg/L Hg2+. These results indicate that Hg2+ may induce apoptotic cell death at low concentrations but not at the highest concentration of 1.0 mg/L. The highest percentage of cells with comet level of 4 was observed in 1.0 mg/L Hg2+. However, Hg2+ is genotoxic to the microalga even at low concentration. In conclusion, Hg2+ can exert its toxic effects on C. vulgaris by changing the microalgal morphology, damaging the microalgal DNA and inducing cell death in the microalgal cells using different pathways at different concentrations.
format Article
author Hazlina A.Z.,
Devanthiran L.,
Fatimah H.,
spellingShingle Hazlina A.Z.,
Devanthiran L.,
Fatimah H.,
Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
author_facet Hazlina A.Z.,
Devanthiran L.,
Fatimah H.,
author_sort Hazlina A.Z.,
title Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_short Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_full Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_fullStr Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_full_unstemmed Morphological changes and DNA damage in Chlorella vulgaris (UMT-M1) induced by Hg2+
title_sort morphological changes and dna damage in chlorella vulgaris (umt-m1) induced by hg2+
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2019
url http://journalarticle.ukm.my/12772/1/48_01_05.pdf
http://journalarticle.ukm.my/12772/
http://mabjournal.com/index.php?option=com_content&view=article&id=897&catid=59:current-view&Itemid=56
_version_ 1643738883849977856
score 13.211869

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