Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene
Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore,...
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Penerbit Universiti Kebangsaan Malaysia
2018
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my-ukm.journal.127522019-04-04T11:34:32Z http://journalarticle.ukm.my/12752/ Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp. (99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species levels. Penerbit Universiti Kebangsaan Malaysia 2018-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12752/1/47_06_05.pdf Nazia Abdul Kadar, and Sitti Raehanah Muhamad Shaleh, and Emma Su Ali, (2018) Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene. Malaysian Applied Biology, 47 (6). pp. 41-45. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56 |
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Microalgae are microscopic organisms that are usually identified based on the morphological features. However traditional
classification are complicated by high levels of morphological plasticity and convergence that will lead to uncertain identification
especially at the species level. Therefore, molecular marker is advantageous to distinguish and correctly identify microalgae at
the lowest taxonomic level based on the genetic information and phylogenetic analyses. In the present study, we have
characterized five strains of microalgae that were deposited in Borneo Marine Research Institute based on 18S rDNA gene
sequences. The BLAST results of microalgae sequences showed high percentage similarities that revealed the species as
Chaetoceros gracilis (99.77%), Thalassiosira weissflogii (99.70%), Nannochloropsis oceanica (100%) and Isochrysis sp.
(99.97%). Combination of both classical and genetic approach is vital to clearly identify microalgae at generic and species
levels. |
| format |
Article |
| author |
Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, |
| spellingShingle |
Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| author_facet |
Nazia Abdul Kadar, Sitti Raehanah Muhamad Shaleh, Emma Su Ali, |
| author_sort |
Nazia Abdul Kadar, |
| title |
Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| title_short |
Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| title_full |
Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| title_fullStr |
Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| title_full_unstemmed |
Molecular and phylogenetic identification of marine microalgae inferred by 18S rDNA gene |
| title_sort |
molecular and phylogenetic identification of marine microalgae inferred by 18s rdna gene |
| publisher |
Penerbit Universiti Kebangsaan Malaysia |
| publishDate |
2018 |
| url |
http://journalarticle.ukm.my/12752/1/47_06_05.pdf http://journalarticle.ukm.my/12752/ http://mabjournal.com/index.php?option=com_content&view=article&id=862&catid=59:current-view&Itemid=56 |
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1643738878892310528 |
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13.211869 |