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Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study

Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas...

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Main Authors: Faizah Mohd Hanapiah,, Anisah Nordin,, Yusof Suboh,, Noraina AR,, Adibah MR,
Format: Article
Language:English
Published: Pusat Perubatan Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf
http://journalarticle.ukm.my/12689/
http://www.medicineandhealthukm.com/toc/12/2
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spelling my-ukm.journal.126892019-03-17T12:16:50Z http://journalarticle.ukm.my/12689/ Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study Faizah Mohd Hanapiah, Anisah Nordin, Yusof Suboh, Noraina AR, Adibah MR, Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas and Methicillin-resistant Staphylococcus aureus (MRSA). In light of this information, a study was undertaken to detect these bacterial endosymbionts in Acanthamoeba spp. isolated from air-conditioning outlets in wards and operating theatres in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). The presence of these bacteria was screened using primer pair targeting each genus and further confirmed by sequencing analysis. Twenty-nine (80.56 %) Acanthamoeba isolates were found to contain targeted bacterial endosymbiont with at least one genus of bacteria per isolates. Mycobacterium spp. (82.76 %) were the most common bacteria detected, followed by Legionella spp. (65.52 %) and Pseudomonas spp. (62.07 %). No MRSA were detected in any isolates used in this study. Most of the Mycobacterium endosymbionts were non-tuberculous mycobacteria, while only two were part of Mycobacterium tuberculosis complex group. We conclude that most Acanthamoeba have the potential to host various pathogenic bacteria. However, the implication on the pathogenicity of both organisms remains unclear and further investigations are needed. Pusat Perubatan Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf Faizah Mohd Hanapiah, and Anisah Nordin, and Yusof Suboh, and Noraina AR, and Adibah MR, (2017) Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study. Medicine & Health, 12 (2). pp. 286-292. ISSN 2289-5728 http://www.medicineandhealthukm.com/toc/12/2
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Acanthamoeba spp. is a free-living amoeba commonly found in the environment. It is the causative agent of Acanthamoeba keratitis (AK) and granulomatous amoebic encephalitis (GAE). This amoeba is also a host to various bacteria including pathogenic ones such as Mycobacterium, Legionella, Pseudomonas and Methicillin-resistant Staphylococcus aureus (MRSA). In light of this information, a study was undertaken to detect these bacterial endosymbionts in Acanthamoeba spp. isolated from air-conditioning outlets in wards and operating theatres in Universiti Kebangsaan Malaysia Medical Centre (UKMMC). The presence of these bacteria was screened using primer pair targeting each genus and further confirmed by sequencing analysis. Twenty-nine (80.56 %) Acanthamoeba isolates were found to contain targeted bacterial endosymbiont with at least one genus of bacteria per isolates. Mycobacterium spp. (82.76 %) were the most common bacteria detected, followed by Legionella spp. (65.52 %) and Pseudomonas spp. (62.07 %). No MRSA were detected in any isolates used in this study. Most of the Mycobacterium endosymbionts were non-tuberculous mycobacteria, while only two were part of Mycobacterium tuberculosis complex group. We conclude that most Acanthamoeba have the potential to host various pathogenic bacteria. However, the implication on the pathogenicity of both organisms remains unclear and further investigations are needed.
format Article
author Faizah Mohd Hanapiah,
Anisah Nordin,
Yusof Suboh,
Noraina AR,
Adibah MR,
spellingShingle Faizah Mohd Hanapiah,
Anisah Nordin,
Yusof Suboh,
Noraina AR,
Adibah MR,
Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
author_facet Faizah Mohd Hanapiah,
Anisah Nordin,
Yusof Suboh,
Noraina AR,
Adibah MR,
author_sort Faizah Mohd Hanapiah,
title Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_short Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_full Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_fullStr Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_full_unstemmed Molecular detection of bacterial endosymbionts in Acanthamoeba spp.: a preliminary study
title_sort molecular detection of bacterial endosymbionts in acanthamoeba spp.: a preliminary study
publisher Pusat Perubatan Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/12689/1/12_faizah_et_al_pdf_19418.pdf
http://journalarticle.ukm.my/12689/
http://www.medicineandhealthukm.com/toc/12/2
_version_ 1643738860230803456
score 13.211869

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