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Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children

Acute respiratory infections cause significant morbidity and mortality in children. Several new respiratory viruses have been identified and co-detection of multiple viruses is commonly reported. This is part of a prospective study which aims to detect respiratory viruses by multiplex molecular meth...

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Main Authors: Zetti Zainol Rashid,, Lee, Pei Chuan, Umi Kalsom Ali,, Muttaqillah Najihan Abdul Samat,, Tang, Swee Fong
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12664/1/25%20Zetti%20Zainol%20Rashid.pdf
http://journalarticle.ukm.my/12664/
http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
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spelling my-ukm.journal.126642019-03-17T11:52:42Z http://journalarticle.ukm.my/12664/ Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children Zetti Zainol Rashid, Lee, Pei Chuan Umi Kalsom Ali, Muttaqillah Najihan Abdul Samat, Tang, Swee Fong Acute respiratory infections cause significant morbidity and mortality in children. Several new respiratory viruses have been identified and co-detection of multiple viruses is commonly reported. This is part of a prospective study which aims to detect respiratory viruses by multiplex molecular method and conventional methods. Nasopharyngeal aspirate specimens were taken from hospitalised children aged less than 5 years with lower respiratory tract infections. These were tested using viral culture, immunofluorescence and Seegene Anyplex™ II RV16 real-time polymerase chain reaction. From 102 samples, 69 (67.6%) were positive by PCR, 12 (11.8%) positive by culture method and 13 (12.7%) positive by IF. A single viral pathogen was detected in 48 samples (47.1%), while 21 samples (20.6%) had co-detection of 2 to 4 viral pathogens. Respiratory syncytial virus (RSV) was most common, detected in 17 samples (16.7% of all samples), followed by adenovirus and rhinovirus in 16 (15.7%), respectively. Bocavirus was detected in 15, enterovirus in 15, influenza A in 8 and parainfluenza-4 in 4 samples, with highest occurrences in co-detection (12/15, 10/15, 5/8 and 3/4, respectively). RSV was the least likely detected in co-detection (3/17). In PCR-positive samples, 54/69 (78.3%) were patients aged up to 24 months. Molecular methods detect more viral aetiologies than conventional methods, with simultaneous detection of multiple respiratory viruses. More sensitive, specific and rapid tools to determine aetiological agents could be incorporated into diagnostic algorithms of respiratory tract infections. Interpretations, significance, and applicability in clinical practice could be further explored, particularly for patients up to 2 years old. Penerbit Universiti Kebangsaan Malaysia 2018-11 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12664/1/25%20Zetti%20Zainol%20Rashid.pdf Zetti Zainol Rashid, and Lee, Pei Chuan and Umi Kalsom Ali, and Muttaqillah Najihan Abdul Samat, and Tang, Swee Fong (2018) Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children. Sains Malaysiana, 47 (11). pp. 2821-2829. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Acute respiratory infections cause significant morbidity and mortality in children. Several new respiratory viruses have been identified and co-detection of multiple viruses is commonly reported. This is part of a prospective study which aims to detect respiratory viruses by multiplex molecular method and conventional methods. Nasopharyngeal aspirate specimens were taken from hospitalised children aged less than 5 years with lower respiratory tract infections. These were tested using viral culture, immunofluorescence and Seegene Anyplex™ II RV16 real-time polymerase chain reaction. From 102 samples, 69 (67.6%) were positive by PCR, 12 (11.8%) positive by culture method and 13 (12.7%) positive by IF. A single viral pathogen was detected in 48 samples (47.1%), while 21 samples (20.6%) had co-detection of 2 to 4 viral pathogens. Respiratory syncytial virus (RSV) was most common, detected in 17 samples (16.7% of all samples), followed by adenovirus and rhinovirus in 16 (15.7%), respectively. Bocavirus was detected in 15, enterovirus in 15, influenza A in 8 and parainfluenza-4 in 4 samples, with highest occurrences in co-detection (12/15, 10/15, 5/8 and 3/4, respectively). RSV was the least likely detected in co-detection (3/17). In PCR-positive samples, 54/69 (78.3%) were patients aged up to 24 months. Molecular methods detect more viral aetiologies than conventional methods, with simultaneous detection of multiple respiratory viruses. More sensitive, specific and rapid tools to determine aetiological agents could be incorporated into diagnostic algorithms of respiratory tract infections. Interpretations, significance, and applicability in clinical practice could be further explored, particularly for patients up to 2 years old.
format Article
author Zetti Zainol Rashid,
Lee, Pei Chuan
Umi Kalsom Ali,
Muttaqillah Najihan Abdul Samat,
Tang, Swee Fong
spellingShingle Zetti Zainol Rashid,
Lee, Pei Chuan
Umi Kalsom Ali,
Muttaqillah Najihan Abdul Samat,
Tang, Swee Fong
Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
author_facet Zetti Zainol Rashid,
Lee, Pei Chuan
Umi Kalsom Ali,
Muttaqillah Najihan Abdul Samat,
Tang, Swee Fong
author_sort Zetti Zainol Rashid,
title Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
title_short Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
title_full Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
title_fullStr Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
title_full_unstemmed Multiplex real-time PCR detection of respiratory viruses in lower respiratory tract infections in children
title_sort multiplex real-time pcr detection of respiratory viruses in lower respiratory tract infections in children
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12664/1/25%20Zetti%20Zainol%20Rashid.pdf
http://journalarticle.ukm.my/12664/
http://www.ukm.my/jsm/malay_journals/jilid47bil11_2018/KandunganJilid47Bil11_2018.html
_version_ 1643738853483216896
score 13.211869

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