Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells

Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the p...

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Main Authors: Manali Haniti Mohd-Zahid,, Juriyati Jalil,, Chan, Kok Meng, Norazrina Azmi,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf
http://journalarticle.ukm.my/12404/
http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
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spelling my-ukm.journal.124042018-12-07T23:38:37Z http://journalarticle.ukm.my/12404/ Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells Manali Haniti Mohd-Zahid, Juriyati Jalil, Chan, Kok Meng Norazrina Azmi, Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the present study, the neuroprotective effects of Ocimum basilicum var. thyrsiflora against hydrogen peroxide (H2O2)-induced oxidative stress in SK-N-SH neuroblastoma cells were evaluated. The exposure of SK-N-SH cells to 50 μM H2O2 for 24 h induced cytotoxicity and apoptosis as measured by cell viability and flow cytometry, respectively. Pretreatment with ethyl acetate (ObEA) fraction at 3.1-25 μg/mL showed the highest protection against H2O2-induced cell death compared to other fractions and crude extract by increasing cell viability and reducing apoptosis. The evaluation of antioxidant capacity via 1, 1-diphenyl-2-picrylahydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays showed ObEA possessed the highest antioxidative properties. The intracellular reactive oxygen species (ROS) production of H2O2 in untreated cells increased by 2.39-fold compared to the control and was significantly attenuated by the 2 h pre-treatment of O. basilicum (p<0.05). The reduction in intracellular superoxide dismutase (SOD) induced by H2O2 was also abrogated by the pretreatment of O. basilicum. These findings suggested that O. basilicum is potentially neuroprotective against oxidative damage in neuronal cells by scavenging free radicals, restoring SOD activities and eventually prevent cell death. Penerbit Universiti Kebangsaan Malaysia 2018-09 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf Manali Haniti Mohd-Zahid, and Juriyati Jalil, and Chan, Kok Meng and Norazrina Azmi, (2018) Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells. Sains Malaysiana, 47 (9). pp. 2129-2139. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease are characterized by the progressive loss of neurons. One of the contributing factors for these diseases is oxidative stress, characterized by the imbalance of free radicals production and antioxidant defense mechanisms. In the present study, the neuroprotective effects of Ocimum basilicum var. thyrsiflora against hydrogen peroxide (H2O2)-induced oxidative stress in SK-N-SH neuroblastoma cells were evaluated. The exposure of SK-N-SH cells to 50 μM H2O2 for 24 h induced cytotoxicity and apoptosis as measured by cell viability and flow cytometry, respectively. Pretreatment with ethyl acetate (ObEA) fraction at 3.1-25 μg/mL showed the highest protection against H2O2-induced cell death compared to other fractions and crude extract by increasing cell viability and reducing apoptosis. The evaluation of antioxidant capacity via 1, 1-diphenyl-2-picrylahydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) assays showed ObEA possessed the highest antioxidative properties. The intracellular reactive oxygen species (ROS) production of H2O2 in untreated cells increased by 2.39-fold compared to the control and was significantly attenuated by the 2 h pre-treatment of O. basilicum (p<0.05). The reduction in intracellular superoxide dismutase (SOD) induced by H2O2 was also abrogated by the pretreatment of O. basilicum. These findings suggested that O. basilicum is potentially neuroprotective against oxidative damage in neuronal cells by scavenging free radicals, restoring SOD activities and eventually prevent cell death.
format Article
author Manali Haniti Mohd-Zahid,
Juriyati Jalil,
Chan, Kok Meng
Norazrina Azmi,
spellingShingle Manali Haniti Mohd-Zahid,
Juriyati Jalil,
Chan, Kok Meng
Norazrina Azmi,
Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
author_facet Manali Haniti Mohd-Zahid,
Juriyati Jalil,
Chan, Kok Meng
Norazrina Azmi,
author_sort Manali Haniti Mohd-Zahid,
title Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_short Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_full Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_fullStr Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_full_unstemmed Neuroprotective effects of Ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in SK-N-SH neuroblastoma cells
title_sort neuroprotective effects of ocimum basilicum extract against hydrogen peroxide-induced oxidative stress in sk-n-sh neuroblastoma cells
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12404/1/22%20Manali%20Haniti%20Mohd-Zahid.pdf
http://journalarticle.ukm.my/12404/
http://www.ukm.my/jsm/english_journals/vol47num9_2018/contentsVol47num9_2018.html
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score 13.211869