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Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii

The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-fol...

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Main Authors: Mok, Pei Shze, Sudesh Kumar,, Liew, Pauline Woan Ying, Jong, Bor Chyan, Najimudin Nazalan,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/12307/1/46_01_14.pdf
http://journalarticle.ukm.my/12307/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56
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spelling my-ukm.journal.123072018-11-16T11:56:27Z http://journalarticle.ukm.my/12307/ Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii Mok, Pei Shze Sudesh Kumar, Liew, Pauline Woan Ying Jong, Bor Chyan Najimudin Nazalan, The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of 3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium by increasing the sucrose concentration from 20 g/L to 25 g/L. Penerbit Universiti Kebangsaan Malaysia 2017-03 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12307/1/46_01_14.pdf Mok, Pei Shze and Sudesh Kumar, and Liew, Pauline Woan Ying and Jong, Bor Chyan and Najimudin Nazalan, (2017) Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii. Malaysian Applied Biology, 46 (1). pp. 93-100. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The production of PHA by Azotobacter vinelandii was characterised in different types of mineral medium including DSMZAzotobacter medium, Ashby’s medium, Burk’s medium and Minimal Medium PHA (MMPHA). A deletion mutant of Azotobacter vinelandii, designated as ΔAvin_16040 was able to accumulate 3-folds higher PHA which was 0.48 ± 0.02 g/L compared to the wild type strain which was 0.11 ± 0.03 g/L in a modified DSMZ-Azotobacter medium. Although this bacterial strain is able to fix nitrogen, addition of urea as external nitrogen source into the medium had increased both its biomass and PHA production. Among the various medium types, MMPHA showed the highest biomass and PHA yields of 3.5 ± 0.1 g/L and 1.50 ± 0.03 g/L, respectively. Higher PHA concentration of 1.74 ± 0.05 g/L was obtained in this medium by increasing the sucrose concentration from 20 g/L to 25 g/L.
format Article
author Mok, Pei Shze
Sudesh Kumar,
Liew, Pauline Woan Ying
Jong, Bor Chyan
Najimudin Nazalan,
spellingShingle Mok, Pei Shze
Sudesh Kumar,
Liew, Pauline Woan Ying
Jong, Bor Chyan
Najimudin Nazalan,
Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
author_facet Mok, Pei Shze
Sudesh Kumar,
Liew, Pauline Woan Ying
Jong, Bor Chyan
Najimudin Nazalan,
author_sort Mok, Pei Shze
title Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_short Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_full Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_fullStr Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_full_unstemmed Characterisation of polyhydroxyalkanoate production by mutant Azotobacter vinelandii
title_sort characterisation of polyhydroxyalkanoate production by mutant azotobacter vinelandii
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/12307/1/46_01_14.pdf
http://journalarticle.ukm.my/12307/
http://www.mabjournal.com/index.php?option=com_content&view=article&id=623&catid=59:current-view&Itemid=56
_version_ 1643738750495227904
score 13.211869

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