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Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA

Traditionally, Clinacanthus nutans (CN) or locally named as ‘Belalai Gajah’ is one of the herbal plant claimed to be able to treat cancer. The aimd of this study are to extract, isolate and characterize the active anticancer compound from CN and to determine the mode of cell death induced by the com...

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Main Authors: Siti Nur Fatihah Mohd Roslan,, Yusmazura Zakaria,, Hasmah Abdullah,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12228/1/17718-76539-1-PB.pdf
http://journalarticle.ukm.my/12228/
http://ejournal.ukm.my/jskm/issue/view/685
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spelling my-ukm.journal.122282018-10-19T21:21:24Z http://journalarticle.ukm.my/12228/ Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA Siti Nur Fatihah Mohd Roslan, Yusmazura Zakaria, Hasmah Abdullah, Traditionally, Clinacanthus nutans (CN) or locally named as ‘Belalai Gajah’ is one of the herbal plant claimed to be able to treat cancer. The aimd of this study are to extract, isolate and characterize the active anticancer compound from CN and to determine the mode of cell death induced by the compound. Bioassay guided fractionation was done on the CN extract by using column chromatography. The cytotoxicity activities of these fractions toward HeLA cells were examined by MTT assay. The nuclear morphology was examined by Hoechst 33258 staining and the cell cycle arrest was evaluated by propium iodide staining using flow cytometry. The presence of active compound in the chosen fraction was determined by Liquid Chromatography Mass Spectrometry (LCMS). Out of 16 fractions collected, Fraction 11(F11) showed the lowest IC50 value with 27 ± 2.6 μg/mL. The value of IC50 for F11 towards normal cell, NIH 3T3 cell and L929 cell, were 70 ± 4.0 μg/mL and 45 ± 1.5 μg/mL respectively. These values were higher than tamoxifen, therefore indicating that tamoxifen is more toxic towards normal cells compared to F11. Nuclear morphology of HeLA cell displayed DNA fragmentation, nuclear condensation and formation of apoptotic bodies upon treatment with F11 for 24 hours. The cell cycle distribution of HeLA cell treated with F11 was arrested at G1 phase. The active compound identified to potentially possess the anticancer property is 19-Oxo-all-trans-retinoic acid. In conclusion, 19-Oxo-all-trans-retinoic acids from F11 of the CN extract, is a potential anticancer agent for cervical cancer. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12228/1/17718-76539-1-PB.pdf Siti Nur Fatihah Mohd Roslan, and Yusmazura Zakaria, and Hasmah Abdullah, (2018) Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA. Jurnal Sains Kesihatan Malaysia, 16 (2). pp. 39-50. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/685
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Traditionally, Clinacanthus nutans (CN) or locally named as ‘Belalai Gajah’ is one of the herbal plant claimed to be able to treat cancer. The aimd of this study are to extract, isolate and characterize the active anticancer compound from CN and to determine the mode of cell death induced by the compound. Bioassay guided fractionation was done on the CN extract by using column chromatography. The cytotoxicity activities of these fractions toward HeLA cells were examined by MTT assay. The nuclear morphology was examined by Hoechst 33258 staining and the cell cycle arrest was evaluated by propium iodide staining using flow cytometry. The presence of active compound in the chosen fraction was determined by Liquid Chromatography Mass Spectrometry (LCMS). Out of 16 fractions collected, Fraction 11(F11) showed the lowest IC50 value with 27 ± 2.6 μg/mL. The value of IC50 for F11 towards normal cell, NIH 3T3 cell and L929 cell, were 70 ± 4.0 μg/mL and 45 ± 1.5 μg/mL respectively. These values were higher than tamoxifen, therefore indicating that tamoxifen is more toxic towards normal cells compared to F11. Nuclear morphology of HeLA cell displayed DNA fragmentation, nuclear condensation and formation of apoptotic bodies upon treatment with F11 for 24 hours. The cell cycle distribution of HeLA cell treated with F11 was arrested at G1 phase. The active compound identified to potentially possess the anticancer property is 19-Oxo-all-trans-retinoic acid. In conclusion, 19-Oxo-all-trans-retinoic acids from F11 of the CN extract, is a potential anticancer agent for cervical cancer.
format Article
author Siti Nur Fatihah Mohd Roslan,
Yusmazura Zakaria,
Hasmah Abdullah,
spellingShingle Siti Nur Fatihah Mohd Roslan,
Yusmazura Zakaria,
Hasmah Abdullah,
Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
author_facet Siti Nur Fatihah Mohd Roslan,
Yusmazura Zakaria,
Hasmah Abdullah,
author_sort Siti Nur Fatihah Mohd Roslan,
title Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
title_short Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
title_full Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
title_fullStr Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
title_full_unstemmed Cytotoxicity of Clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, HeLA
title_sort cytotoxicity of clinacanthus nutans and mechanism of action of its active fraction towards human cervical cancer cell line, hela
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12228/1/17718-76539-1-PB.pdf
http://journalarticle.ukm.my/12228/
http://ejournal.ukm.my/jskm/issue/view/685
_version_ 1643738728594669568
score 13.211869

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