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Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates

Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic...

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Main Authors: Ahmad Sh. A. Lafi,, Jacinta Santhanam,, Tzar Mohd Nizam Khaithir,, Nur Fashya Musa,, Fahrul Huyop,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf
http://journalarticle.ukm.my/12222/
http://ejournal.ukm.my/jskm/issue/view/685
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spelling my-ukm.journal.122222018-10-19T21:09:00Z http://journalarticle.ukm.my/12222/ Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates Ahmad Sh. A. Lafi, Jacinta Santhanam, Tzar Mohd Nizam Khaithir, Nur Fashya Musa, Fahrul Huyop, Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 μg/mg, respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 μg/mg, respectively), and high ergosterol (Candida tropicalis at 22.84 μg/mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples. Penerbit Universiti Kebangsaan Malaysia 2018 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf Ahmad Sh. A. Lafi, and Jacinta Santhanam, and Tzar Mohd Nizam Khaithir, and Nur Fashya Musa, and Fahrul Huyop, (2018) Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates. Jurnal Sains Kesihatan Malaysia, 16 (2). pp. 15-21. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/685
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Ergosterol, a component of fungal cell membrane, has been frequently detected as an indicator of fungal presence and mass in environmental samples like soil. However, its detection in major pathogenic fungal species has not been investigated. In this study, the ergosterol contents of ten pathogenic fungal species were determined. Liquid chromatography was used for the detection and quantification of ergosterol extracted from fungal broth cultures. Results showed that ergosterol eluted as a single, well resolved peak in the chromatogram profiles of all tested fungi. Based upon relative amounts of ergosterol produced per fungal mycelial dry weight, three groups of fungal pathogens were identified, namely low ergosterol (Aspergillus niger, Candida albicans and Cryptococcus neoformans at 4.62, 6.29 and 7.08 μg/mg, respectively), medium ergosterol (Fusarium solani, Aspergillus fumigatus, Mucor sp., Penicillium sp., Cryptococcus gattii and Rhizopus sp. at 9.40, 10.79, 10.82, 11.38, 12.60 and 13.40 μg/mg, respectively), and high ergosterol (Candida tropicalis at 22.84 μg/mg), producers. Ergosterol was not detectable in bacterial samples, which were included as controls. This first report on ergosterol detection in major pathogenic fungal species indicates that ergosterol may be used as a biomarker to diagnose invasive fungal infections in clinical samples.
format Article
author Ahmad Sh. A. Lafi,
Jacinta Santhanam,
Tzar Mohd Nizam Khaithir,
Nur Fashya Musa,
Fahrul Huyop,
spellingShingle Ahmad Sh. A. Lafi,
Jacinta Santhanam,
Tzar Mohd Nizam Khaithir,
Nur Fashya Musa,
Fahrul Huyop,
Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
author_facet Ahmad Sh. A. Lafi,
Jacinta Santhanam,
Tzar Mohd Nizam Khaithir,
Nur Fashya Musa,
Fahrul Huyop,
author_sort Ahmad Sh. A. Lafi,
title Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_short Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_full Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_fullStr Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_full_unstemmed Determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
title_sort determination of ergosterol as a potential biomarker in pathogenic medically important fungal isolates
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12222/1/18923-76535-1-PB.pdf
http://journalarticle.ukm.my/12222/
http://ejournal.ukm.my/jskm/issue/view/685
_version_ 1643738727718060032
score 13.211869

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