Cover Image

Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands

Nicotine is claimed to increase free radicals, DNA damage and lipid peroxidation in male reproductive organs. Nigella sativa has been identified to ameliorate these adverse effects due to nicotine intake. This study was conducted to evaluate potential protective effects of N. sativa oil on the adver...

Full description

Saved in:
Bibliographic Details
Main Authors: Lina S.,, Eliza H.,, Hashida N.H.,, Ibrahim S.F.,, Osman K.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12206/1/22%20Lina%2C%20S.pdf
http://journalarticle.ukm.my/12206/
http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-ukm.journal.12206
record_format eprints
spelling my-ukm.journal.122062018-10-16T13:03:41Z http://journalarticle.ukm.my/12206/ Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands Lina S., Eliza H., Hashida N.H., Ibrahim S.F., Osman K., Nicotine is claimed to increase free radicals, DNA damage and lipid peroxidation in male reproductive organs. Nigella sativa has been identified to ameliorate these adverse effects due to nicotine intake. This study was conducted to evaluate potential protective effects of N. sativa oil on the adverse effects of nicotine on androgen receptors (AR) and ultrastructural features of rat seminal vesicles and prostate glands. Twenty four Sprague Dawley male rats, 7-9 weeks of age and 200-250 g body weight (BW) were randomly divided into; Group 1 Saline (S), forced fed with 0.1 mL/100 g BW of 0.9% normal saline; Group 2 Nicotine (N), intramuscularly injected with 0.5 mg/100 g BW of nicotine; Group 3 N. sativa (NS), forced fed with 6.0 μL/100 g BW of N. sativa and Group 4 Nicotine-N. sativa (NNS), co-administered with 0.5 mg/100 g BW of nicotine and 6.0 μL/100 g BW of N. sativa. The seminal vesicles and prostate glands were extracted after 100 days of treatment. The seminal vesicle and prostate gland were processed for ultrastructural study and androgen receptor detection. The epithelial cells in prostate gland and seminal vesicle of the N group showed weaker brown staining intensity as compared to that of in the NS and NNS groups. This was consistent with the presence of some ultracellular changes observed in the prostate gland and seminal vesicle tissues of the N group. Findings from this study suggested that administration of N. sativa results in ameliorating effects on both the prostate gland and seminal vesicle structures and functions of the nicotine-treated rats. Penerbit Universiti Kebangsaan Malaysia 2018-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12206/1/22%20Lina%2C%20S.pdf Lina S., and Eliza H., and Hashida N.H., and Ibrahim S.F., and Osman K., (2018) Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands. Sains Malaysiana, 47 (8). pp. 1827-1833. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Nicotine is claimed to increase free radicals, DNA damage and lipid peroxidation in male reproductive organs. Nigella sativa has been identified to ameliorate these adverse effects due to nicotine intake. This study was conducted to evaluate potential protective effects of N. sativa oil on the adverse effects of nicotine on androgen receptors (AR) and ultrastructural features of rat seminal vesicles and prostate glands. Twenty four Sprague Dawley male rats, 7-9 weeks of age and 200-250 g body weight (BW) were randomly divided into; Group 1 Saline (S), forced fed with 0.1 mL/100 g BW of 0.9% normal saline; Group 2 Nicotine (N), intramuscularly injected with 0.5 mg/100 g BW of nicotine; Group 3 N. sativa (NS), forced fed with 6.0 μL/100 g BW of N. sativa and Group 4 Nicotine-N. sativa (NNS), co-administered with 0.5 mg/100 g BW of nicotine and 6.0 μL/100 g BW of N. sativa. The seminal vesicles and prostate glands were extracted after 100 days of treatment. The seminal vesicle and prostate gland were processed for ultrastructural study and androgen receptor detection. The epithelial cells in prostate gland and seminal vesicle of the N group showed weaker brown staining intensity as compared to that of in the NS and NNS groups. This was consistent with the presence of some ultracellular changes observed in the prostate gland and seminal vesicle tissues of the N group. Findings from this study suggested that administration of N. sativa results in ameliorating effects on both the prostate gland and seminal vesicle structures and functions of the nicotine-treated rats.
format Article
author Lina S.,
Eliza H.,
Hashida N.H.,
Ibrahim S.F.,
Osman K.,
spellingShingle Lina S.,
Eliza H.,
Hashida N.H.,
Ibrahim S.F.,
Osman K.,
Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
author_facet Lina S.,
Eliza H.,
Hashida N.H.,
Ibrahim S.F.,
Osman K.,
author_sort Lina S.,
title Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
title_short Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
title_full Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
title_fullStr Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
title_full_unstemmed Androgen receptor and ultrastructural features of Nigella sativa oil and nicotine-treated male rat reproductive glands
title_sort androgen receptor and ultrastructural features of nigella sativa oil and nicotine-treated male rat reproductive glands
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12206/1/22%20Lina%2C%20S.pdf
http://journalarticle.ukm.my/12206/
http://www.ukm.my/jsm/english_journals/vol47num8_2018/contentsVol47num8_2018.html
_version_ 1643738723038265344
score 13.211869

Similar Items