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Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis

Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus com...

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Main Authors: Safiyyah Shahimi,, Sahilah Abd. Mutalib,, Wan Sakeenah Wan Nazri,, Aminah Abdullah,, Norrakiah Abdullah Sani,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf
http://journalarticle.ukm.my/12171/
http://www.ukm.my/jsm/english_journals/vol47num7_2018/contentsVol47num7_2018.html
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spelling my-ukm.journal.121712018-10-09T20:57:56Z http://journalarticle.ukm.my/12171/ Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis Safiyyah Shahimi, Sahilah Abd. Mutalib, Wan Sakeenah Wan Nazri, Aminah Abdullah, Norrakiah Abdullah Sani, Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Seven endonucleases of AluI, BsaJI, HaeIII, HindIII, HinfI, MboI and MboII were examined for the ability to digest cyt b amplicon from each species. Genomic DNA of pork (Sus scrofa domestica) were differentiated from fishes by comparing the digestion patterns produced by similar amplified region and enzymes used. In the present study, it was demonstrated that fishes and pork DNA genome were successfully differentiated using all endonucleases except for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA detection in fish products. Penerbit Universiti Kebangsaan Malaysia 2018-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf Safiyyah Shahimi, and Sahilah Abd. Mutalib, and Wan Sakeenah Wan Nazri, and Aminah Abdullah, and Norrakiah Abdullah Sani, (2018) Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis. Sains Malaysiana, 47 (7). pp. 1535-1540. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num7_2018/contentsVol47num7_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Genomic DNA of 13 fish (n=13) species consist of four freshwater which were catfish (Clarias gariepinus), shark catfish (Pangasius larnaudii), tilapia (Oreochromis mossambicus), perch (Lates calcarifer) and nine marine species which were black pomfret (Parastromateus niger), anchovy (Stolephorus commersonii), mabong (Rastrelliger kanagurta), red snapper (Lutjanus erythropterus), herring (Chirocentrus dorab), ray fish (Himantura gerrardii), sardine (Decapterus macrosoma), mackerel (Euthynnus affinis) and tuna (Thunnus tuna) were differentiated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Seven endonucleases of AluI, BsaJI, HaeIII, HindIII, HinfI, MboI and MboII were examined for the ability to digest cyt b amplicon from each species. Genomic DNA of pork (Sus scrofa domestica) were differentiated from fishes by comparing the digestion patterns produced by similar amplified region and enzymes used. In the present study, it was demonstrated that fishes and pork DNA genome were successfully differentiated using all endonucleases except for HindIII. Thus, PCR-RFLP analysis was found useful for future pork DNA detection in fish products.
format Article
author Safiyyah Shahimi,
Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Aminah Abdullah,
Norrakiah Abdullah Sani,
spellingShingle Safiyyah Shahimi,
Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Aminah Abdullah,
Norrakiah Abdullah Sani,
Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
author_facet Safiyyah Shahimi,
Sahilah Abd. Mutalib,
Wan Sakeenah Wan Nazri,
Aminah Abdullah,
Norrakiah Abdullah Sani,
author_sort Safiyyah Shahimi,
title Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_short Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_full Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_fullStr Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_full_unstemmed Comparison of DNA profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) analysis
title_sort comparison of dna profiling between fishes and pork meat using polymerase chain reaction-restriction fragment length polymorphisms (pcr-rflp) analysis
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12171/1/22%20Safiyyah%20Shahimi.pdf
http://journalarticle.ukm.my/12171/
http://www.ukm.my/jsm/english_journals/vol47num7_2018/contentsVol47num7_2018.html
_version_ 1643738713336840192
score 13.211869

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