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Effect of gamma irradiation on the physical stability of DPPC liposomes

Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range...

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Main Authors: Liyana Mohd Ali Napia,, Irman Abdul Rahman,, Mohd Yusof Hamzah,, Faizal Mohamed,, Hur Munawar Kabir Mohd,, Intan Syakeela Ahmad Bastamam,, Shamellia Sharin,, Norsyahidah Mohd Hidzir,, Shahidan Radiman,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf
http://journalarticle.ukm.my/12128/
http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
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spelling my-ukm.journal.121282018-09-28T22:36:31Z http://journalarticle.ukm.my/12128/ Effect of gamma irradiation on the physical stability of DPPC liposomes Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman, Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability. Penerbit Universiti Kebangsaan Malaysia 2018-06 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf Liyana Mohd Ali Napia, and Irman Abdul Rahman, and Mohd Yusof Hamzah, and Faizal Mohamed, and Hur Munawar Kabir Mohd, and Intan Syakeela Ahmad Bastamam, and Shamellia Sharin, and Norsyahidah Mohd Hidzir, and Shahidan Radiman, (2018) Effect of gamma irradiation on the physical stability of DPPC liposomes. Sains Malaysiana, 47 (6). pp. 1235-1240. ISSN 0126-6039 http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability.
format Article
author Liyana Mohd Ali Napia,
Irman Abdul Rahman,
Mohd Yusof Hamzah,
Faizal Mohamed,
Hur Munawar Kabir Mohd,
Intan Syakeela Ahmad Bastamam,
Shamellia Sharin,
Norsyahidah Mohd Hidzir,
Shahidan Radiman,
spellingShingle Liyana Mohd Ali Napia,
Irman Abdul Rahman,
Mohd Yusof Hamzah,
Faizal Mohamed,
Hur Munawar Kabir Mohd,
Intan Syakeela Ahmad Bastamam,
Shamellia Sharin,
Norsyahidah Mohd Hidzir,
Shahidan Radiman,
Effect of gamma irradiation on the physical stability of DPPC liposomes
author_facet Liyana Mohd Ali Napia,
Irman Abdul Rahman,
Mohd Yusof Hamzah,
Faizal Mohamed,
Hur Munawar Kabir Mohd,
Intan Syakeela Ahmad Bastamam,
Shamellia Sharin,
Norsyahidah Mohd Hidzir,
Shahidan Radiman,
author_sort Liyana Mohd Ali Napia,
title Effect of gamma irradiation on the physical stability of DPPC liposomes
title_short Effect of gamma irradiation on the physical stability of DPPC liposomes
title_full Effect of gamma irradiation on the physical stability of DPPC liposomes
title_fullStr Effect of gamma irradiation on the physical stability of DPPC liposomes
title_full_unstemmed Effect of gamma irradiation on the physical stability of DPPC liposomes
title_sort effect of gamma irradiation on the physical stability of dppc liposomes
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12128/1/19%20Liyana%20Mohd%20Ali%20Napia.pdf
http://journalarticle.ukm.my/12128/
http://www.ukm.my/jsm/malay_journals/jilid47bil6_2018/KandunganJilid47Bil6_2018.html
_version_ 1643738700972032000
score 13.211869

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