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Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method

The isolation method for dental pulp stem cells (DPSCs) is still unclear to obtain a conducive environment for DPSCs to proliferate. Enzymatic digestion and outgrowth method are two commonly used methods for DPSCs isolation but are not well characterized in mice DPSCs. This study aimed to compare...

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Main Authors: Farinawati Yazid,, Nur Atmaliya Luchman,, Rohaya Megat Abdul Wahab,, Shahrul Hisham Zainal Ariffin,, Sahidan Senafi,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2018
Online Access:http://journalarticle.ukm.my/12053/1/UKM%20SAINSMalaysiana%2047%2804%29Apr%202018%2006.pdf
http://journalarticle.ukm.my/12053/
http://www.ukm.my/jsm/english_journals/vol47num4_2018/contentsVol47num4_2018.html
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spelling my-ukm.journal.120532018-09-16T23:07:00Z http://journalarticle.ukm.my/12053/ Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method Farinawati Yazid, Nur Atmaliya Luchman, Rohaya Megat Abdul Wahab, Shahrul Hisham Zainal Ariffin, Sahidan Senafi, The isolation method for dental pulp stem cells (DPSCs) is still unclear to obtain a conducive environment for DPSCs to proliferate. Enzymatic digestion and outgrowth method are two commonly used methods for DPSCs isolation but are not well characterized in mice DPSCs. This study aimed to compare these isolation methods and differentiation potential of mice DPSCs into bone cells. Dental pulp was extracted from mice’s incisors and subjected to isolation either by collagenase 1A or culture of pulp tissue in complete alpha-Modified Eagle Medium (αMEM). Both cells isolated were cultured until passage 4 and subjected to in vitro proliferation and differentiation analysis. Both cells exhibited fibroblastliked morphology, but cells isolated by enzyme digestion proliferate faster compare to outgrowth method. After 21 days of osteoblast differentiation, DPSCs isolated from enzyme digestion method showed alkaline phosphatase (ALP) activity slightly different as compared to outgrowth method. In conclusion, there is a significant difference between the cells isolated from enzyme digestion compare to outgrowth method with regard to proliferation and osteoblast differentiation. Thus, it is preferable to isolate by enzyme digestion as it is faster and consistent compared to outgrowth method. Penerbit Universiti Kebangsaan Malaysia 2018-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12053/1/UKM%20SAINSMalaysiana%2047%2804%29Apr%202018%2006.pdf Farinawati Yazid, and Nur Atmaliya Luchman, and Rohaya Megat Abdul Wahab, and Shahrul Hisham Zainal Ariffin, and Sahidan Senafi, (2018) Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method. Sains Malaysiana, 47 (4). pp. 691-698. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol47num4_2018/contentsVol47num4_2018.html
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description The isolation method for dental pulp stem cells (DPSCs) is still unclear to obtain a conducive environment for DPSCs to proliferate. Enzymatic digestion and outgrowth method are two commonly used methods for DPSCs isolation but are not well characterized in mice DPSCs. This study aimed to compare these isolation methods and differentiation potential of mice DPSCs into bone cells. Dental pulp was extracted from mice’s incisors and subjected to isolation either by collagenase 1A or culture of pulp tissue in complete alpha-Modified Eagle Medium (αMEM). Both cells isolated were cultured until passage 4 and subjected to in vitro proliferation and differentiation analysis. Both cells exhibited fibroblastliked morphology, but cells isolated by enzyme digestion proliferate faster compare to outgrowth method. After 21 days of osteoblast differentiation, DPSCs isolated from enzyme digestion method showed alkaline phosphatase (ALP) activity slightly different as compared to outgrowth method. In conclusion, there is a significant difference between the cells isolated from enzyme digestion compare to outgrowth method with regard to proliferation and osteoblast differentiation. Thus, it is preferable to isolate by enzyme digestion as it is faster and consistent compared to outgrowth method.
format Article
author Farinawati Yazid,
Nur Atmaliya Luchman,
Rohaya Megat Abdul Wahab,
Shahrul Hisham Zainal Ariffin,
Sahidan Senafi,
spellingShingle Farinawati Yazid,
Nur Atmaliya Luchman,
Rohaya Megat Abdul Wahab,
Shahrul Hisham Zainal Ariffin,
Sahidan Senafi,
Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
author_facet Farinawati Yazid,
Nur Atmaliya Luchman,
Rohaya Megat Abdul Wahab,
Shahrul Hisham Zainal Ariffin,
Sahidan Senafi,
author_sort Farinawati Yazid,
title Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
title_short Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
title_full Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
title_fullStr Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
title_full_unstemmed Proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
title_sort proliferation and osteoblast differentiation mice dental pulp stem cells between enzyme digestion and outgrowth method
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2018
url http://journalarticle.ukm.my/12053/1/UKM%20SAINSMalaysiana%2047%2804%29Apr%202018%2006.pdf
http://journalarticle.ukm.my/12053/
http://www.ukm.my/jsm/english_journals/vol47num4_2018/contentsVol47num4_2018.html
_version_ 1643738679870488576
score 13.211869

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