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Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis

Fusarium species are known to cause various diseases on plantations including fruits and vegetables. The most common Fusarium that can cause plant diseases are Fusarium proliferatum and Fusarium verticillioides. Ear rot disease on maize, wilt disease on cucurbits and fruit rot disease on tomato as w...

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Main Authors: Najihah A.,, Nur Ain Izzati M.Z.,, Yong, S.Y.C., Nik Mohd Izham M.N.,
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:http://journalarticle.ukm.my/11710/1/18%20A.%20Najihah.pdf
http://journalarticle.ukm.my/11710/
http://www.ukm.my/jsm/english_journals/vol46num12_2017/contentsVol46num12_2017.htm
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spelling my-ukm.journal.117102018-05-30T00:28:34Z http://journalarticle.ukm.my/11710/ Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis Najihah A., Nur Ain Izzati M.Z., Yong, S.Y.C. Nik Mohd Izham M.N., Fusarium species are known to cause various diseases on plantations including fruits and vegetables. The most common Fusarium that can cause plant diseases are Fusarium proliferatum and Fusarium verticillioides. Ear rot disease on maize, wilt disease on cucurbits and fruit rot disease on tomato as well as banana are example of diseases caused by these two species. The objectives of this study were to identify F. proliferatum and F. verticillioides based on species-specific primers and polymerase chain reaction (PCR) amplification and to evaluate the genetic diversity of both species based on microsatellite markers. Fifty isolates of Fusarium species that were previously collected throughout Malaysia from different hosts were identified by using species-specific PCR amplification. Twenty-nine isolates were identified as F. proliferatum and 21 isolates were identified as F. verticillioides based on species-specific primer. The genetic diversity of all the fungal isolates was evaluated by using microsatellite analysis with six established primers. Five out of six primers amplified polymorphic bands with the most effective primer showing high polymorphism were (AG)7C and (TCC)5 meanwhile one primer (TTTC)4 gave negative result with no band amplified. The phylogenetic tree that was constructed showing two different clades distinguished between F. proliferatum and F. verticillioides. Penerbit Universiti Kebangsaan Malaysia 2017-12 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11710/1/18%20A.%20Najihah.pdf Najihah A., and Nur Ain Izzati M.Z., and Yong, S.Y.C. and Nik Mohd Izham M.N., (2017) Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis. Sains Malaysiana, 46 (12). pp. 2425-2432. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol46num12_2017/contentsVol46num12_2017.htm
institution Universiti Kebangsaan Malaysia
building Perpustakaan Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Fusarium species are known to cause various diseases on plantations including fruits and vegetables. The most common Fusarium that can cause plant diseases are Fusarium proliferatum and Fusarium verticillioides. Ear rot disease on maize, wilt disease on cucurbits and fruit rot disease on tomato as well as banana are example of diseases caused by these two species. The objectives of this study were to identify F. proliferatum and F. verticillioides based on species-specific primers and polymerase chain reaction (PCR) amplification and to evaluate the genetic diversity of both species based on microsatellite markers. Fifty isolates of Fusarium species that were previously collected throughout Malaysia from different hosts were identified by using species-specific PCR amplification. Twenty-nine isolates were identified as F. proliferatum and 21 isolates were identified as F. verticillioides based on species-specific primer. The genetic diversity of all the fungal isolates was evaluated by using microsatellite analysis with six established primers. Five out of six primers amplified polymorphic bands with the most effective primer showing high polymorphism were (AG)7C and (TCC)5 meanwhile one primer (TTTC)4 gave negative result with no band amplified. The phylogenetic tree that was constructed showing two different clades distinguished between F. proliferatum and F. verticillioides.
format Article
author Najihah A.,
Nur Ain Izzati M.Z.,
Yong, S.Y.C.
Nik Mohd Izham M.N.,
spellingShingle Najihah A.,
Nur Ain Izzati M.Z.,
Yong, S.Y.C.
Nik Mohd Izham M.N.,
Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
author_facet Najihah A.,
Nur Ain Izzati M.Z.,
Yong, S.Y.C.
Nik Mohd Izham M.N.,
author_sort Najihah A.,
title Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
title_short Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
title_full Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
title_fullStr Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
title_full_unstemmed Characterization of Fusarium proliferatum and Fusarium verticillioides based on species-specific gene and microsatellites analysis
title_sort characterization of fusarium proliferatum and fusarium verticillioides based on species-specific gene and microsatellites analysis
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2017
url http://journalarticle.ukm.my/11710/1/18%20A.%20Najihah.pdf
http://journalarticle.ukm.my/11710/
http://www.ukm.my/jsm/english_journals/vol46num12_2017/contentsVol46num12_2017.htm
_version_ 1643738578852773888
score 13.211869

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